GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual page 40

5 Operation
5.1 Assemble transfer stack
Step
2
3
4
5
Illustration of a transfer stack
with multiple gels
The following illustration shows a parallel transfer stack.
40
Action
Remove all trapped air by rolling a clean pipet or roller from the center toward
the edges.
Place the pre-wet membrane onto the stack of blotting paper.
Place the gel on the membrane.
NOTICE
Take care to place the gel correctly on the first try be-
cause proteins begin to transfer immediately; once
transfer has begun, moving the gel will distort results
or cause "shadow" bands on the blot.
Cover the gel with three layers of saturated blotting paper.
NOTICE
Make sure that there is no electrical contact between the two
stacks.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA