Waters 2998 Overview And Maintenance Manual page 12

A.7 Electrical and handling symbols ................................................................................................ 76
A.7.1 Electrical symbols.......................................................................................................... 76
A.7.2 Handling symbols .......................................................................................................... 77
B Specifications ....................................................................................................... 79
B.1 Physical specifications .............................................................................................................. 79
B.2 Environmental specifications ..................................................................................................... 79
B.3 Electrical specifications ............................................................................................................. 79
B.4 Performance specifications ....................................................................................................... 80
B.5 Optical component specifications ............................................................................................. 81
B.6 Flow cell specifications ............................................................................................................. 82
C Solvent Considerations ....................................................................................... 83
C.1 Introduction ............................................................................................................................... 83
C.1.1 Clean solvents............................................................................................................... 83
C.1.2 Solvent quality............................................................................................................... 83
C.1.3 Preparation checklist ..................................................................................................... 83
C.1.4 Water............................................................................................................................. 83
C.1.5 Use buffers .................................................................................................................... 84
C.1.6 Tetrahydrofuran ............................................................................................................. 84
C.2 Solvent miscibility ..................................................................................................................... 84
C.2.1 How to use miscibility numbers..................................................................................... 85
C.3 Buffered solvents ...................................................................................................................... 86
C.4 Head height .............................................................................................................................. 86
C.5 Minimum bend radius for tubing ............................................................................................... 86
C.6 Solvent viscosity ....................................................................................................................... 87
C.7 Mobile phase solvent degassing ............................................................................................... 87
C.7.1 Gas solubility................................................................................................................. 87
C.8 Solvent degassing methods ...................................................................................................... 88
C.8.1 Sparging ........................................................................................................................ 88
C.8.2 Vacuum degassing ........................................................................................................ 88
C.8.3 Solvent degassing considerations................................................................................. 89
C.9 Wavelength selection ................................................................................................................ 89
C.9.1 UV cutoffs for common solvents.................................................................................... 89
C.9.2 Mixed mobile phases .................................................................................................... 90
December 5, 2017, 715004753 Rev. B
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