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Appendix E: Sample/Slide Preparation Guidelines
D.4 General Guidelines
• Load specimens of similar type in the same carousel.
• Since cytocentrifuged body fluid preparations are deposited in a monolayer, stain intensity setting
should be less than that for a smear.
• Higher settings are best for smears since it takes longer for the stain to penetrate to the bottom of a
multi-layer of cells.
D.5 Staining Cytocentrifuged Preparations
NOTE: For best results, always use coated slides (poly-L-lysine SS-118)
Use one of the following methods of fixation:
• Use spray fixative and allow to air dry for a minimum of 15 minutes.
• Immerse in 95% alcohol for up to 30 minutes, but no less than 10 minutes.
• Immerse in a Sacammanno type fixative for up to 30 minutes but no less than 15 minutes.
NOTE: If cytocentrifuged sample display area is thick with cells, the probability of the sample being
dislodged is very high. A new cytoprep with a cell count decreased would be advisable.
Suggested Staining Settings
1. Use the No Pre-Wash setting.
2. Use lower Nuclear/Htox intensity setting.
3. Use lower Cytoplasmic/EU intensity setting.
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