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Section 6: Nozzle Maintenance and Performance
6.4 Performing the Volume Test
The Volume Test requires the Nozzle Maintenance Kit.
NOTE: The Volume Test must be performed weekly.
Table 11: Volume Test Tolerances
Nozzle/Reagent Line
A
B
C
D
E
F
NOTE: The stainer normally functions correctly if nozzle volumes are slightly higher or lower than the
specified range. Spray volumes < 7.0 mL or > 11.0 mL indicate serious problems with the nozzles or
reagent delivery lines.
• If the volume is within the tolerance range, go to Step 9.
• If the volume is outside the tolerance range:
A. Clear the nozzle orifice with the nozzle brush found in the maintenance kit. Press the individual
bristles into the nozzle openings.
B. If necessary, remove the nozzle and perform the Nozzle Cleaning procedure (Section 6.1).
C. If the problem persists, replace the nozzle.
NOTE: If the problem persists after replacing the nozzle, contact ELITechGroup.
1. From the Maintenance menu, select Volume Test.
2. Hold a Volume Test tube (small tube) to cover the selected nozzle.
3. Press the corresponding reagent button to collect the reagent.
NOTE: With QC/Maintenance tracking enabled, enter the measured volume on
the keypad and press ENTER. With QC/Maintenance Tracking disabled, the
menu returns to the Maintenance menu.
4. Remove and cap the tube.
5. Record the nozzle position on the tube.
6. Place the tube in the appropriate position in the tube stand.
7. Repeat Steps 2 through 6 for each nozzle.
8. Compare collected nozzle volumes with the following table.
Minimum
9.0 mL
9.0 mL
9.0 mL
7.5 mL
9.0 mL
9.0 mL
Maximum
11.0 mL
11.0 mL
11.0 mL
10.0 mL
11.0 mL
11.0 mL
65
65
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