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Section 4: Operating the Stainer
4.1 Operating Instructions
Suggested Staining Protocol
NOTE: Samples and slides should be prepared and fixed according to recommendations in Appendix E or
equivalent.
• Perform a Hub Pattern Test (once per day).
• Select or verify desired stain program.
• If slide tracking is enabled, scan or enter slide information.
• Load slides into the carousel. Use blocking slides if needed.
• Place loaded carousel into the stainer and close the lid.
• Check reagent and waste levels.
• If slide tracking is not enabled, enter the number of slides on the Main menu.
• Perform a stain cycle.
• Unload the carousel.
Performing a Hub Pattern Test
Use the Hub Pattern Test to ensure the nozzles are clear of debris and spraying properly.
1. From the Maintenance menu, press Pattern Test.
2. Hold a sheet of white paper towel near the drive hub, squarely facing the target nozzle.
3. Press the corresponding button for the reagent line to be tested.
4. Check the pattern. If incorrect, clean the nozzle orifice with the nozzle brush provided in the Nozzle
Maintenance Kit. If this fails to correct the problem, refer to Nozzle Maintenance and Performance
(Section 6).
Figure 7: Correct Hub Pattern Test Result
Figure 8: Incorrect Hub Pattern Test Result
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