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Appendix D: Stain Modes and Programming Options
Wash Settings
Acid Alcohol Wash
Increase numbers to reduce hematoxylin background staining. Do not set to 0 unless extra maintenance
will be performed on nozzles. To conserve reagent, select minimum value which produces satisfactory
results.
Stain Setting
Spray Applications
Wait Applications
Bluing Wash
Increase numbers to better blue the hematoxylin in the nucleus. To conserve reagent, select minimum
value which produces satisfactory results.
Stain Setting
Spray Applications
Wait Applications
Orange G Wash
Removes excess OG reagent in preparation for application of EA reagent. To conserve reagent, select
minimum value which produces satisfactory results.
Stain Setting
Spray Applications
Wait Applications
Final Wash
Removes excess EA reagent at end of staining. To conserve reagent, select minimum value which
produces satisfactory results.
Stain Setting
Spray Applications
Wait Applications
1
2
3
4
1
2
3
3
0
0
0
3
1
2
3
4
2
3
4
5
0
0
0
0
1
2
3
4
2
3
4
5
0
0
0
0
1
2
3
4
9
12
12
15
0
0
0
0
5
6
7
8
4
4
6
6
4
7
16
15
5
6
7
8
6
7
8
9
0
0
0
0
5
6
7
8
6
7
8
9
0
0
0
0
5
6
7
8
15
21
27
33
9
21
36
48
9
7
16
9
10
0
9
10
0
9
45
72
91
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