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Appendix D: Stain Modes and Programming Options
D.3 Examples of Program Settings with Staining Results
Following are examples of buccal specimens prepared using a smear technique and stained using different
program setting. The sides were cleared and then cover slipped and viewed at a magnification of 10x.
Table 15: Program Settings with Staining Results 1 and 2
Program Setting
Figure 1 Figure 2
Prewash Alcohol
1
Prewash Bluing
0
Nuclear/Htox
1
Acid Alcohol Wash
3
Bluing
1
Bluing Wash
2
Orange G
1
Orange G Wash
2
Cytoplasmic/EA
1
Final Wash
2
End Spin
0
Figure 1
1
0
1
9
1
2
1
2
1
2
0
Figure 2
93
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