Appendix A: Reagents (Stains, Washes, Agents); Aerospray® Cytology Reagents - Elitech AEROSPRAY CYTOLOGY 7522 User Manual

Appendix A: Reagents (Stains, Washes, Agents)

Aerospray Cytology Reagents
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ELITechGroup Aerospray Cytology reagents are proprietary Papanicolaou staining reagents intended for
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use in the Aerospray Cytology stainer in cytology and histology labs.
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Hematoxylin Stain
ELITechGroup offers Hematoxylin I (SS-051D) and II (SS-051D2). Both hematoxylin stains can be used
for either progressive or regressive staining functions. They are suitable for use with all body fluid types
intended to be stained with the Cytology stainer. Choice of Hematoxylin and settings would be based on
individual laboratory preferences.
Bluing Agent
The Cytology bluing agent (SS-051C) is a low molarity basic solution which raises the pH of the cells,
causing the nucleus to turn blue. While there is no water rinse after the bluing step, no precipitate is formed
due to the low concentration of this basic solution.
Orange G Stain
The Orange G stain solution (SS-051B) is specifically formulated for staining with the pre-programmed
staining times of the Cytology stainer.
EA-50 Stain
EA-50 is a counter stain used for cytoplasmic staining (SS-051A). It is specifically formulated for staining
with the pre-programmed staining times of the Cytology stainer.
Alcohol Wash
The Cytology stainer utilizes a proprietary blend of alcohols (SS-051E) to dehydrate and clear the cells.
Acid Alcohol Wash
The Cytology stainer utilizes a proprietary formula (SS-051F) which allows for more control of Hematoxylin
stain intensity. Typically, acid-alcohol is used when applying a regressive stain methodology.
WARNING!
To avoid serious instrument damage, never use reagents containing organic solvents unless
supplied by ELITechGroup or specified in official ELITechGroup formulation instructions.
See Appendix C for ordering information.
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