Prepare Vials For Sampling; Spiking A Vial - Teledyne Tekmar Versa User Manual

prepare Vials for sampling

1. Place the cap on a firm surface.
2. Insert the septum into the cap (avoid contamination of the septum face, the shiny Teflon® face should always be
face-down toward the vial).
3. Place the septum and cap together on top of the vial opening.
4. Place the crimper on top of the capped vial with the crimp head resting on the cap.
5. Squeeze the crimper handles firmly in a slow and smooth motion, do not squeeze quickly.
6. Remove the crimper.
7. Check for a proper crimp seal by lightly rotating the cap. If some resistance exists, it is properly sealed.

spiking a Vial

Spiking a vial containing a fortification solution such as an internal standard, surrogate standard, or stock standard can
be done prior to capping or after the vial is sealed. Both techniques have advantages and disadvantages.
Versa User Manual
Crimper
Septum
(Teflon® face
down toward
vial opening)
Figure 3-4: Hand Crimper and Vial
Installation & Setup • Page 3-4
Cap
Sample Vial
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