Standard Split/Splitless And Standard On-Column Sampling Modes - Agilent Technologies Varian CP-8400 Operator's Manual

4. Solvent plug (if defined)
5. Upper Internal Standard Air Gap (if defined)
6. Internal Standard (if defined)
7. Upper Sample Air Gap (if defined)
8. Sample
9. Air Plug After Sample (if defined)
There are six liquid injection modes (Standard On-Column, Standard
Split/Splitless, Neat, Volatile, Viscous, User Defined) and SPME–
Selecting one of the five predefined modes or SPME will cause
predefined AutoSampler methods to be used. These methods are
defined as follows.
Standard Split/Splitless and Standard On-Column Sampling
Modes
Internal Standard is not permitted with Standard Sampling modes.
Standard mode parameters are set for samples dissolved in common
solvents such as iso-octane.
The Standard sampling mode performs the following sequence of
actions:
1. Any pre-injection Clean actions will occur.
2. The syringe is positioned in the sample vial according to the
3. The volume of sample specified in the Single Method or Method
4. The AutoSampler will inject the entire contents into the specified
5. The post injection clean flushes will be executed.
36
Sample Penetration Depth setting. The syringe plunger is
withdrawn and the Syringe contents expelled back into the vial
five times at the set plunger withdrawal Speed and the maximum
plunger expel speed.
Sequence Automation will be withdrawn from the specified
sample vial. The sled will ascend to the top of its travel and the
plunger will be withdrawn an amount equal to 1 µL (Air Plug after
Sample).
injector. Standard On-Column mode injects at 5 µL/second.
Standard Split/Splitless mode injects at 50 µL/second with 10 µL
syringe.
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