Section 9: Operating The Visualization System (Video Display) Introduction; Operating Instructions; Standard Slide Preparation; Testing Capillary Preparation - MES SQA-V Gold User Manual

User Guide Version 2.60 I-button WHO 5th
Visualization
SECTION 9: Operating the Visualization System (Video Display)
System
The SQA-V Visualization System with video display (upper screen) is used to view and
count sperm cells. The visualization system is a critical "link" to V-Sperm GOLD where
enhanced, real time video can be displayed on a PC monitor. The visualization system:
Operating

Standard Slide Preparation:

Instructions

Testing Capillary Preparation:

Visualization Process:

Counting Cells Using the Visualization Screen:

Revision 21_Sep_2015

Accommodates both an SQA-V testing capillary to "scan" through a depth of
300 microns or a standard slide to view samples (20 micron depth).

Operates via control knobs to set focus, brightness, contrast and color, and via
the keypad zoom, illumination, and monitor on/off functions.

Magnification range: x300 to x500.

Use 10 µl of semen.

Standard slide, 22 mm x 22 mm cover-slip (to insure 20 micron depth).

Load the prepared, standard slide into the SQA-V slide adaptor.

Fill the SQA-V testing capillary for either a normal or low volume specimen
(see Appendix).

The video display will automatically illuminate when the SQA-V is turned on.

Use monitor ON/OFF key on the keypad to independently operate the video
display.

Wait for the self-test to complete (system is disabled at this time).

To ensure that the visualization system is working properly prior to use:

Press the HIGH ILLUMINATION key multiple times to ensure a
maximum level setting.

To view cells:

To count cells:

Insert semen sample (either capillary or slide) into the visualization chamber.

Adjust CONTRAST, COLOR, BRIGHTNESS, FOCUS and object
ILLUMINATION controls for optimal image quality.

Use ZOOM OUT (x300) / ZOOM IN (x500) to regulate magnification.
1. Follow the WHO Manual instructions for semen sample collection and
preparation. Thoroughly mix the sample before step #2.
2. Pipette 10uL of the semen sample onto a standard slide and cover with a
22x22 mm coverslip. Prepare a new slide if air bubbles or liquid spillage
occurs.
3. Load the slide into the slide adaptor and then insert the slide adaptor into the
SQA-V visualization chamber. (Refer to the SQA-V User Guide APPENDIX 3:
Using Standard Slides in the Visualization System for details).
4. Press the ZOOM-OUT button on the SQA-V keypad all the way to set the
magnification to x300.
Press ZOOM IN to maximum magnification (x500).
Press ZOOM OUT to minimum magnification (x300).
27