Postvasectomy Test - MES SQA-V Gold User Manual

User Guide Version 2.60 I-button WHO 5th
Post

Postvasectomy Test

Vasectomy
The SQA-V runs a five minute POSTVASECTOMY test that can detect the presence of a
Testing
very small number of motile cells. Once the automated test has been performed, the
user is given the option to follow the POSTVASECTOMY protocol outlined below and
"scan" the testing capillary in the SQA-V visualization system (A POSTVACECTOMY
Protocol can also be found in the appendix section of this guide).
By scanning through the depth of the testing capillary, immotile and motile sperm cells
can be readily identified, easily counted and entered in the operational screen for visual
confirmation of the automated test results. Clinical studies positively demonstrated that
by incorporating both the SQA-V automated AND visualization system in the testing
protocol, a very high level of accuracy is obtained for identifying motile and non-motile
sperm cells in POSTVASECTOMY samples.
In order to obtain similar levels of accuracy it is imperative that the user strictly follow
the manufacturer's protocol outlined below. Additionally, once the testing cycle is
completed, test results can be documented by capturing and archiving a video clip of the
postvasectomy specimen using V-Sperm™ software.
Please note:
The POSTVASECTOMY
test takes
approximately 5
minutes to run and is
highly sensitive to
motion. Please do not
disturb the SQA-V or
the testing capillary
during the testing cycle
or the results may be
impacted.
Revision 21_Sep_2015
Select POSTVASECTOMY as the SAMPLE TYPE from the ENTER PATIENT / SAMPLE
DATA screen.

Fill the SQA-V testing capillary following instructions in the appendix section of
this guide: "Filling the SQA-V Capillary with a Normal Volume Sample."

Insert the testing capillary into the SQA-
V lower chamber when instructed.
Testing will begin automatically.

Testing takes approximately 5 minutes.

Test results for motile sperm are
reported.

Select YES to when asked: "ENTER
VISUAL DATA PER USER GUIDE?" to
manually enter the number of
MOTILE/IMMOTILE sperm seen on the
visualization system.

Press ENTER to continue.

Take the same testing capillary and
insert it into the visualization (upper)
compartment.

Set the magnification to x300 (Full zoom
out) and press ENTER to continue.

"Scan" the depth of the capillary by
slightly turning the visualization focus
knob (10 fields can be visualized) and
enter the total # MOTILE/IMMOTILE
SPERM cells visualized in all 10 fields.

The SQA-V will automatically report the
GREATER # of cells found by the
Automated or Visualization system.

Press ENTER and the test results screen
will be displayed.
DO NOT MOVE CAPILLARY OR
OPERATE DEVICE DURING TESTING
THIS TEST TAKES APPROX. 5 MINUTES
# SPERM/SCAN: # SPERM/SAMPLE VOL.:
MOTILE
ENTER VISUAL DATA PER USER GUIDE?
PLEASE INSERT CAPILLARY
ADJUST MAGNIFICATION TO x300
TURN FOCUS KNOB AND SCAN THROUGH
ENTIRE CAPILLARY DEPTH TO COUNT
MOTILE AND IMMOTILE SPERM
# MOTILE SPERM
# IMMOTILE SPERM
19
TESTING
POSTVASECTOMY
3 MOTILE 0.2 M
YES/NO
INTO VISUALIZATION SLOT
PRESS ENTER
PLEASE ENTER:
3
8