Instructions For Kingfisher Duo With 12-Pin Magnet Head - Thermo Scientific KingFisher Pure RNA Blood Kit Instruction Manual

Chapter 5
| Protocols and Pipetting Instructions
20 | Thermo Scientific KingFisher Pure RNA Blood Kit
When the KingFisher Flex pauses at the second dispense step after the
DNase step at approximately 10 minutes after the first dispense step,
remove the DNase plate from the instrument, and add the Rebinding Buffer
(supplemented with ethanol) to the DNase plate to rebind the RNA.
Plate name
DNase
9. Place the DNase plate back into the instrument and press Start. After
the pause, the protocol will continue to completion.
10. When the protocol is completed, remove the plates according to the
instructions on the KingFisher Flex display and switch off the instrument.
The purified RNA is ready for use in downstream applications. When
working with RNA, keep the purified samples on ice. Store the purified
RNA at -20°C or -80°C.
NOTE: The final RNA concentration in the nuclease-free water may
increase if the purified RNA is eluted into a smaller than recommended
volume of water, but this can slightly reduce the overall RNA yield.
Instructions for KingFisher Duo with 12-pin
Magnet Head
These instructions are intended for RNA purification from 200 µl of blood,
using the KingFisher Pure RNA Blood Kit (Cat. No. 98020196) and the
KingFisher Duo with 12-pin magnet head.
When using the KingFisher Pure RNA Blood Kit for the first time, prepare
the DNase I storage solution, Rebinding Buffer, Wash Buffer 1, and Wash
Buffer 2. For each run, prepare the DNase I working solution and Lysis Buffer
with 2 M DTT. For more instructions, refer to Chapter 4:
and Preparation of the
Check all the solutions in the kit for salt precipitation before each use.
Redissolve precipitates by warming the solution at 37°C and equilibrate to
room temperature (15−25°C).
1. Take one empty Microtiter deep well 96 plate and one Thermo Scientific™
KingFisher™ Duo elution strip.
Add
Rebinding Buffer
Reagents".
Added reagent volume
per well
490 µl
"Storage Conditions
Thermo Fisher Scientific