Thermo Scientific KingFisher Flex User Manual
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Thermo Scientific KingFisher Flex User Manual

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Thermo Scientific
KingFisher Flex
User Manual
Rev. 1.5, Cat. no. N07669

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  • Page 1 Thermo Scientific KingFisher Flex User Manual Rev. 1.5, Cat. no. N07669...
  • Page 2 US 6447729, Method and means for magnetic particle specific binding assay, and US 6448092, Separation device for microparticles involving a magnetic rod. KingFisher Flex also has national and international patents pending. Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments.

  • Page 3: Intended Users

    This user manual is designed to give you the information to: user manual Review safety precautions  Install the KingFisher Flex  Use the KingFisher Flex in routine jobs – the processing step  Perform basic cleaning and maintenance procedures  Troubleshoot the instrument performance ...

  • Page 4: Safety Symbols And Markings

    European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EC. A black label with the following text (Figure 2–4): WARNING: DISCONNECT SUPPLY BEFORE SERVICING AVERTISSEMENT: COUPER L'ALIMENTATION AVANT L'ENTRETIEN ET LE DEPANNAGE 4 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 5: Warning And Other Markings Used In The Documentation

    Note Marks a hint, important information that is useful in the optimum operation of the system, or an item of interest. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 6: Instrument Safety And Guidelines For Use

    The device shall be operated only with software specifically  designed for the device. Never open any other covers of the KingFisher Flex than  the transparent lid or the sliding door (Figure 2–3) while the instrument is plugged into a power source.

  • Page 7: Table Of Contents

    For more information ................. 3 Safety symbols and markings ..............4 Safety symbols and markings used on the KingFisher Flex ....4 Warning and other markings used in the documentation ...... 5 Instrument safety and guidelines for use ..........6 Chapter 1 Introduction to the KingFisher Flex ............
  • Page 8 Contents Do’s and Don’ts of the KingFisher Flex ........... 39 Chapter 4 Routine Operation ..................39 Do ......................39 Don’t ..................... 39 Switching on .................... 40 Control panel ................... 40 Keypad ....................40 Keys ....................40 Display ....................41 Navigating ..................... 41 Using KingFisher Flex PC software ............
  • Page 9 List of accessories and consumables............81 List of spare parts ..................82 Chapter 10 References ....................83 Keywords for web pages ................. 83 Literature ....................84 Appendix A Certificate of Decontamination ............... 87 Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 10 Figure 1–1. KingFisher Flex magnetic particle processor ......13 Figure 2–2. KingFisher Flex front view without transparent lid ....15 Figure 2–3. KingFisher Flex front view with transparent lid and plates ..15 Figure 2–4. KingFisher Flex back view ............16 Figure 2–5.
  • Page 11 Figure 4–44. Tip comb in the tip comb holder ........... 57 Figure 4–45. Shield plate in function ............57 Figure 4–46. KingFisher Flex plates ............58 Figure 4–47. Inserting the heating block ............ 59 Figure 4–48. Pressing the heating block into place ........59 Figure 4–49.
  • Page 12 Table 8–9. Actions taken against error messages and warnings....78 Table 9–10. Codes for products ..............81 Table 9–11. Codes for accessories and consumables ......81 Table 9–12. Codes for spare parts ............. 82 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 13: Introduction To The Kingfisher Flex

    The patented technology of the KingFisher Flex system is based on the use of magnetic rods covered with a disposable, specially designed tip comb and plates. The instrument functions without any dispensing or aspiration parts or devices.

  • Page 14: Advantages Of Using Kingfisher Flex

    Introduction to the KingFisher Flex Advantages of using KingFisher Flex Advantages of The KingFisher Flex magnetic particle processor has several operational using KingFisher advantages: Flex  Enables automation of complicated manual steps  Enables simultaneous processing and purification  Binding and elution are enhanced due to the heating option Enables concentration of the sample during processing ...

  • Page 15: Chapter 2 Functional Description

    Chapter 2 Functional Description Instrument This section shows the front, back and side views of the KingFisher Flex layout instrument. Front view The front views of the KingFisher Flex instrument are shown in Figure 2–2 and Figure 2–3. Interchangeable KingFisher Flex head Shield plate Plate station (1–8)

  • Page 16: Back / Side View

    Figure 2–4. KingFisher Flex back view Figure 2–5. KingFisher Flex side view KingFisher Flex The KingFisher Flex (Figure 2–6) has room for eight plates. The tip combs magnetic are compatible with the plates. During the individual steps, the plates are...

  • Page 17: Principle Of Magnetic Particle Processing

    Magnetic particles are transferred with the aid of magnetic rods covered with a disposable, specially designed plastic tip comb. Figure 2–6. KingFisher Flex magnetic particle processor Principle of magnetic particle processing Figure 2–7. Inverse magnetic particle processing...

  • Page 18: Working With A Magnetic Rod

    The volume of the first plate can be larger than the volume of the next during the magnetic plate, and this is used for concentration purposes (see Figure 2–8 below). particle processing Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 19: Figure 2-8. A Concentration Step During Magnetic Particle Processing

    Functional Description Working with a magnetic rod Figure 2–8. A concentration step during magnetic particle processing Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 20 Functional Description Working with a magnetic rod Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 21: Chapter 3 Installation

    Doing so might cause misalignment and will void the instrument warranty. Warning The KingFisher Flex weighs approximately 28 kg [62 lbs.] without the transport package and should be lifted with care. It is recommended that two persons lift the instrument together, taking proper precautions to avoid injury.

  • Page 22: Checking Delivery

    If any parts are damaged, contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy. Requirements When you set up your KingFisher Flex, avoid sites of operation with excess dust, vibrations, strong magnetic fields, direct sunlight or UV light, draft, excessive moisture or large temperature fluctuations.

  • Page 23: Precautions And Limitations

    KingFisher Flex heads. Do not hold the KingFisher Flex heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex heads.

  • Page 24: Figure 3-9. Kingfisher Flex Transport Locks Fitted

    Heating block transport lock Figure 3–9. KingFisher Flex transport locks fitted 1. To remove the transport lock of the tip comb holder, take hold of the transport lock screw and firmly turn it counterclockwise according to Figure 3–10 Figure 3–10.

  • Page 25: Figure 3-11. Tip Comb Holder Transport Lock Removed

    . Unscrew the transport lock screw counterclockwise Figure 3–12 Figure 3–13 ). Lift the screw with the label attached ( Figure 3–12. Removing the transport lock of the heating block (A) Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 26: How To Ensure Startup

    Figure 2–4 back/side panel ( ) corresponds to the local voltage. 2. Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 27: How To Fit The Subassemblies Of The Instrument Into Place

    How to fit the subassemblies This section describes the installation setups of the shield plate, the heating of the instrument into place block, the interchangeable KingFisher Flex heads and the transparent lid. Shield plate To install the shield plate, follow these steps: 1.

  • Page 28: Figure 3-16. Inserting The Shield Plate

    4. When fastening the two plate retaining screws ( ), be Figure 3–18 careful not to drop them inside the instrument. shows the shield plate installed. Figure 3–17. Fastening / loosening the shield plate Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 29: Heating Block

    ( Figure 3–20 ). The heating block will snap into place at both ends if you have done it correctly. Figure 3–19. Inserting the heating block Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 30: Figure 3-20. Pressing The Heating Block Into Place

    All KingFisher Flex plates can be heated. There are four different kinds of interchangeable heating blocks available, for KingFisher 24 deep well plates, Thermo Scientific™ Microtiter™ deep well 96 plates, KingFisher 96 plates, and PCR plates (Table 3–1). Table 3–1. Plates vs. heating adapter(s)

  • Page 31: Interchangeable Kingfisher Flex Heads

    Figure 3–21. Sectional view of the Microtiter deep well 96 plate (a), deep well 96 plates of other manufacturers (b, c, d) and the KingFisher Flex 96 deep well heating block You can only use the following 24 and 96-well plates for heating: KingFisher 24 deep well plate (200 µl –...

  • Page 32: Figure 3-22. Kingfisher Flex Heads In Their Storage Boxes

    KingFisher Flex heads. Do not hold the KingFisher Flex heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex heads.

  • Page 33: Figure 3-23. Inserting The Kingfisher Flex Head

    Maintenance menu (Maintenance > Maintenance protocol > Change_magnet) by using the up and down cursor keys. Then press . Insert the KingFisher Flex head so that the three pins (shown with START black arrows) slot into place (Figure 3–23 and Figure 3–24). When you insert the KingFisher Flex head, be careful not to damage the magnet rods against the tip comb holder frame.

  • Page 34: Transparent Lid

    Installation Operational check Figure 3–24. KingFisher Flex head for KingFisher 24 deep well plate fitted Transparent lid During operation, the sliding door can be open or closed or the whole Figure 3–25 transparent lid can be absent ( ). The closed door protects the processing against environmental contamination.

  • Page 35: How To Pack The Instrument For Transportation

    Run the check protocol (Check_96dw_tip, Check_KF96_tip, Check_pcr_tip, or Check_24dw_tip) under the Maintenance menu according to the KingFisher Flex head and plastic consumables you are using. If the check is all right, proceed with your own runs.

  • Page 36: How To Refit The Transport Lock Of The Tip Comb Holder

    Figure 1. Press the tip comb holder downwards evenly with both hands ( 3–28 Caution Ensure that the power is switched off. Figure 3–28. Pressing the tip comb holder downwards Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 37: Figure 3-29. Location Of The Locking Hole

    Then fasten the screw by turning it firmly Figure 3–30 clockwise ( Locking hole Figure 3–29. Location of the locking hole Figure 3–30. Fitting the transport lock of the tip comb holder Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 38: Figure 3-31. Both Transport Locks Refitted

    Installation How to pack the instrument for transportation Figure 3–31. Both transport locks refitted Figure 3–31 shows both the transport lock of the heating block and the tip comb holder refitted. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 39: Do's And Don'ts Of The Kingfisher Flex

    Doing so might cause misalignment and will invalidate the instrument warranty.  Never open any other cover of the KingFisher Flex than the transparent Figure 2–3 lid or the sliding door ( ) while the instrument is plugged into a power source.

  • Page 40: Switching On

    Routine Operation Switching on Switching on Before you switch on the KingFisher Flex, ensure that the voltage on the type label at the bottom left of the back/side panel (Figure 2–4) corresponds to the local voltage. Warning Never operate your instrument from a power outlet that has no ground connection.

  • Page 41: Display

    Main view row Icon Info text bar Figure 4–33. Main view on the display of the KingFisher Flex There are three menus in the KingFisher Flex user interface: Factory protocols, User protocols and Maintenance. In routine use you mainly navigate in the Factory/User protocols menu. In advanced level options there is one extra menu, Maintenance.
  • Page 42 To move from one menu to another, make sure you are in the main view of one of the menus and use the Left and Right arrow keys. The main views of each menu tab are shown below. User protocols Factory protocols Maintenance Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 43: Table 4-3. Icons In The Main View

    “Selecting the protocol” on page 44 DNA/RNA Cell Protein Other “Device information” on page 47 “Computer interface” on page 48 “Language” on page 48 “Buzzer” on page 49 “Maintenance protocol” on page 50 Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 44: Using Kingfisher Flex Pc Software

    Routine Operation Using KingFisher The operation of the KingFisher Flex magnetic particle processor can also Flex PC software be controlled by an external computer and run on BindIt Software. In addition to the KingFisher Flex internal software features, you can also download protocols to the instrument or back up protocols from one instrument and transfer them to another.

  • Page 45: Setting The Default Protocol

    If you do not, the last used protocol will be shown. To set the default protocol, go to the Last used/Default protocol row. Last used Default Press to start setting your default protocol. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 46 Factory / User protocols Press to select your default protocol. Use the Up and Down arrow keys to select the protocol. Press to set the default protocol. Press to go back. STOP Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 47: Instrument Options

    Device information The device information shows the name of the instrument, the internal software version and the serial number of the instrument unit. Go to the Maintenance menu. Press Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 48: Computer Interface

    Use the Up and Down arrow keys to select the computer interface in use. Not active will reset the computer interfaces. Press Language You can set the language of the internal software. Go to the Maintenance menu. Select the Language selection row. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 49: Buzzer

    You can choose whether or not the instrument produces a sound for different functions. Note that all functions make a certain sound. Go to the Maintenance menu. Select the Buzzer on/off row. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 50: Maintenance Protocol

    Press Maintenance protocol You can set which protocol is automatically selected as the maintenance protocol when the KingFisher Flex is powered on. Go to the Maintenance menu. Select the Maintenance protocol row. Press . All available maintenance protocols will be displayed.

  • Page 51: How To Handle Tip Combs

    Figure 4–35 2. Unpack the package by separating the two tip combs ( and placing one of the tip combs onto the KingFisher 96 plate Figure 4–39 Figure 4–40 (= tip-plate) ( Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 52: Figure 4-35. Separating The Two Tip Combs

    Figure 4–36. Storing the other tip comb on a KingFisher 96 plate for further To handle PCR tip combs correctly, follow these steps. 1. Take a PCR tip comb package containing eight tip combs within each Figure 4–37 other on a screen plate ( Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 53: Figure 4-37. Pcr Tip Comb Package

    3. Before you take the tip comb into use, press the tip comb evenly against the enclosed 24 deep well plate with the aid of a magnetic head or another object. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 54: How To Start

    KingFisher plate (= tip-plate) per run ( 4–40 ). Confirm the plate loading by pressing START Figure 4–39. Combining the tip comb and KingFisher plate (A) Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 55: Figure 4-40. Combining The Tip Comb And Kingfisher Plate (B)

    Flex head. The plate station position depends on the protocol used. After the protocol has been run, note that the turntable may stop in a different position than the basic position. A1 corner Figure 4–41. Loading the tip-plate Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 56: Figure 4-42. Loading The Plates

    Figure 4–42. Loading the plates Magnetic rods Tip comb holder Tip-plate Figure 4–43. Plates loaded 4. The tip comb is automatically locked onto the tip comb holder from the Figure 4–44 tip-plate ( Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 57: Figure 4-44. Tip Comb In The Tip Comb Holder

    ( ) forming a protective cover. Shield plate Figure 4–45. Shield plate in function 6. Close the sliding door. The transparent lid (if in place) protects the instrument against environmental contamination. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 58: Changing The Heating Block

    Figure 4–46. KingFisher Flex plates Caution Do not use other plates than the ones listed in Table 4–4. Other plates may not be compatible with the KingFisher Flex heating blocks. They may also cause unexpected problems, such as cross-contamination due to the divergent well volume and bottom height of the plate.

  • Page 59: Figure 4-47. Inserting The Heating Block

    You can add a heating step of ambient temperature +5C to +115C to a protocol. The heating block is preheatable but no cooling can be carried out. All KingFisher Flex plates can be heated. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 60: Shutdown

    Shutdown To shut down the KingFisher Flex, follow the steps below: 1. Switch the KingFisher Flex off by pressing the power switch (Figure 2–4) at the bottom left of the back/side panel of the instrument into the OFF position.

  • Page 61: Emergency Situations

    3. Carry out appropriate corrective measures. However, do not disassemble the instrument. 4. If the corrective measures taken do not help, contact authorized technical service or your local Thermo Fisher Scientific representative. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 62 Routine Operation Emergency situations Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 63: Chapter 5 Maintenance

    Although the KingFisher Flex is constructed from high-quality materials, you must immediately wipe away spilled saline solutions, solvents, acids or alkaline solutions from outer surfaces to prevent damage.

  • Page 64: Figure 5-50. Unfastening The Two Finger Screws

    Clean the black spill shield (Figure 5–52) and the turntable using a soft cloth or tissue paper soaked in a mild detergent solution (SDS), soap solution or alcohol. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 65: How To Clean The Magnetic Rods

    KingFisher Flex heads. Do not hold the KingFisher Flex heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex heads.

  • Page 66: Disposal Of Materials

    1. Prepare the decontaminant: 200 ml 4% glutaraldehyde solution (or another agent recommended by your safety officer). 2. Empty the turntable. Figure 3. Switch OFF the power and disconnect the mains supply cable ( 3–14 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 67: Packing For Service

     Decontaminate the instrument beforehand. Install the transport locks.  Place the KingFisher Flex head into its transportation box.  Pack the instrument according to the enclosed packing instructions.  Use the original packaging to ensure that no damage will occur to the ...

  • Page 68: Service Contracts

    Contact the Thermo Fisher Scientific technical service department for more details. Disposal of the If the KingFisher Flex has to be disposed of, follow the guidelines below. instrument Warning Decontaminate the instrument before disposal. Refer to “Decontamination procedure” on page 66 about decontamination.

  • Page 69: Chapter 6 Technical Specifications

    Table 6–7. Performance specifications Performance specifications 24: 200 µl – 5 mL Processing volume • 200–500 µl performance may vary depending on the mixing speed and liquid 96: 20–1000 µl Capacity 96 samples/run Continued Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 70: Safety Specifications

    START PAUSE STOP TURNTABLE ROTATION CLOCKWISE TURNTABLE / four cursor keys / LCD ROTATION COUNTERCLOCKWISE This section describes the safety specifications for the KingFisher Flex Safety instrument. specifications In conformity KingFisher Flex bears the following markings: with the Type 711 requirements 100–240 Vac, 50/60 Hz, 175 VA...
  • Page 71 2) The pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only nonconductive pollution, such as dust, occurs with the exception of occasional conductivity caused by condensation. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 72 Technical Specifications In conformity with the requirements Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 73: Frequently Asked Questions

    KingFisher 24 deep well plates, Microtiter deep well 96 plates, KingFisher 96 plates, and PCR plates. Each plate type has an optimized KingFisher Flex head and tips. Of the four plates, the KingFisher 96 plate can be used with all three kinds...
  • Page 74 KingFisher Flex heads. Do not hold the KingFisher Flex heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex heads.
  • Page 75 Q15: Are there any limitations in the use of mixing speeds? Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 76 Q17: What should you do if the tip loading does not succeed? A17: Try first to manually stretch the tips both lengthwise and width wise to level the tip comb. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 77: Chapter 8 Troubleshooting Guide

    Error message Description – No error The command was not recognized The command was not recognized as a valid KingFisher Flex as a valid command command. The tip comb holder lifting The magnetic head position is in error. mechanism is out of position The turntable rotating mechanism The turntable position is in error.

  • Page 78: Troubleshooting Guide

    Timer already expired. This warning is reported if a wait for a timer (WAI) command is executed and the timer has already expired. Troubleshooting A troubleshooting guide for the KingFisher Flex instrument is guide presented in Table 8–9. Table 8–9. Actions taken against error messages and...
  • Page 79 It is recommended that you clean up the program memory from unnecessary protocols about once a month to prevent this error from occurring. Warning Description Code Timer expired Contact authorized technical service. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 80 Troubleshooting Guide Troubleshooting guide Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 81: Chapter 9 Ordering Information

    Instrument / System KingFisher Flex – 96 PCR head 5400610 KingFisher Flex – 96 KF head 5400620 KingFisher Flex – 96 deep well head 5400630 KingFisher Flex – 24 deep well plate 5400640 Table 9–11. Codes for accessories and consumables...

  • Page 82: List Of Spare Parts

    Item Quantity block 97002514 KingFisher Flex 96 tip comb for PCR 80 pcs magnets 97002524 KingFisher Flex 96 tip comb for KF 96 100 pcs magnets 97002534 KingFisher Flex 96 tip comb for DW 100 pcs magnets 97002610 KingFisher Flex 24 deep well tip comb...

  • Page 83: Chapter 10 References

    His-tag proteins separation immunomagnetic separation silica-coated particles immunoprecipitation streptavidin-coated beads KingFisher streptavidin-coated particles magnetic beads superparamagnetic beads magnetic capture superparamagnetic microspheres magnetic microspheres Thermo Fisher Scientific magnetic particles Thermo Scientific magnetic separation Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...

  • Page 84: Literature

    (GATA) 7, 145–150. Jakobsen, K. S., Breivold, E. and Hornes, E. (1990): Purification of mRNA directly from crude plant tissues in 15 minutes using oligo dT microspheres. Nucleic Acids Res. 18, 3669. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...
  • Page 85 Robino, C., Barilaro, M. B., Gino, S., Chiarle, R., Palestro, G. and Torre C. (2006): Incestuous paternity detected by STR- typing of chorionic villi isolated from archival formalin-fixed paraffin-embedded abortion material using laser microdissection. Journal of Forensic Sciences 51, 90–92. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 85...
  • Page 86 Ye, J.-D., Tereshko,V., Frederiksen, J. K., Koide, A., Fellouse, F. A., Sidhu, S. S., Koide, S., Kossiakoff, A. A. and Piccirilli, J. A. (2008): Synthetic antibodies for specific recognition and crystallization of structured RNA. PNAS 105, 82–87. Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

  • Page 87: Appendix A Certificate Of Decontamination

    *) The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials. This unit is certified by the undersigned to be free of radioactive contamination. Date and place: Signature: Name (block capitals): Please include decontaminating solution used. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 88 Certificate of Decontamination Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...
  • Page 89 KingFisher 96 plates, and PCR always has to be placed onto the plates (Figure 4–49). microplate prior to processing. There are KingFisher Flex head There are four kinds four different kinds of disposable tip of interchangeable KingFisher Flex heads combs available: available, for KingFisher 24 deep well ...
  • Page 90 Glossary: 90 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...
  • Page 91 65 incubation, 18 shield plate, 66 installation, 3, 21, 22, 23, 27, 67, 71 turntable, 63 installing the KingFisher Flex, 21, 23, 71 cleaning the magnetic rods, 65 instrument layout, 15 cleaning the shield plate, 66 instrument options, 47...
  • Page 92 25, 26, 35, 36 66, 68, 74, 78, 79 warranty, 2, 21, 22, 39 working with a magnetic rod, 18 samples, 13, 14, 16, 23, 66, 69, 74, 75, 89 92 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...
  • Page 93 Notes Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual...
  • Page 94 Notes 94 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific...

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