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Summary of Contents for Thermo Scientific KingFisher Plant DNA Kit
- Page 1 Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Rev. 1.1...
- Page 3 Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Rev. 1.1, Cat. no. N11996...
- Page 4 Copyright © 2011 Thermo Fisher Scientific Inc. First edition published in 2010. All rights reserved. Reproduction of the accompanying user documentation in whole or in part is prohibited. “BindIt”, “KingFisher”, “Microtiter”, “Multidrop”, “NanoDrop” and "Versette" are registered trademarks of Thermo Fisher Scientific. All other trademarks and registered trademarks are the property of their respective holders.
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Page 5: Table Of Contents
Quantification and determination of the purity of DNA ..29 Chapter 6 General Information.................31 Reagent specificity and volumes ..........31 Handling of magnetic beads ............31 Binding and wash steps ............31 Elution step ................32 Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual... - Page 6 Contents Appendix A Troubleshooting ................35 Appendix B Ordering Information ...............37 Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 7: Kit Content
Chapter 1 Kit Content Table 1-1. Thermo Scientific KingFisher Plant DNA Kit Item KingFisher Plant DNA Kit Cat. No. 97050196 Package size 1 x 96 samples KingFisher Magnetic Beads 3.2 ml Lysis Buffer 55 ml Binding Buffer 50 ml Wash Buffer 1... - Page 8 50 pcs 95040460 Microtiter deep well 96 plate, sterile 50 pcs 95040470 KingFisher Flex 24 deep well plate 50 pcs 95040480 KingFisher Flex 24 deep well plate, sterile 50 pcs Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
- Page 9 50 pcs 97002090 KingFisher plastics 100 µl 8-pack, 8 plates and 8 tip combs 1 box 97002094 KingFisher plastics 200 µl 8-pack, 8 plates and 8 tip combs 1 box Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual...
- Page 10 Kit Content Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 11: Chapter 2 Product Description
DNA from plant materials using KingFisher instruments. The DNA purified with the KingFisher Plant DNA Kit is of high quality and free of proteins, nucleases, and other contaminants or inhibitors. It is therefore suitable for direct use in many different... -
Page 12: Kit Specifications
Heat incubation is included in the elution step when using the KingFisher Flex or KingFisher Duo. Kit specifications The KingFisher Plant DNA Kit is designed for rapid automated preparation of highly pure DNA from plant materials using KingFisher magnetic particle processors. -
Page 13: Kingfisher Magnetic Particle Processors
Thermo Scientific Multidrop Combi and/or the Thermo Scientific Versette. Thermo Scientific BindIt Software 3.2 can be used for running ready-made and optimized protocols for the Thermo Scientific KingFisher Kits. It is also possible to transfer the defined protocol onto the onboard software and run it directly from the instrument. - Page 14 Product Description KingFisher magnetic particle processors The KingFisher Plant DNA Kit is optimized and ready for use with KingFisher systems. The KingFisher magnetic particle processors are intended for professional research use by trained personnel. Detailed information and user instructions for the KingFisher instruments can be found in their respective user manuals.
- Page 15 No heating available temperature The BindIt Software 3.2 protocol optimized for the KingFisher Plant DNA Kit is available for the KingFisher Flex or KingFisher Duo. BindIt Software 3.2 protocols for the Thermo Scientific KingFisher 96, the KingFisher and the Thermo Scientific KingFisher mL are available on request.
- Page 16 Product Description KingFisher magnetic particle processors Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 17: Chapter 3 Safety Information
Sodium perchlorate < 15% Flammable. and ethanol < 24% RNase A Lyophilized RNase A May cause sensitization by inhalation and skin contact. Keep container tightly closed. Do not inhale dust. Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual... - Page 18 Safety Information Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 19: Chapter 4 Storage Conditions And Preparation Of The Working Solution
The buffers are ready for use. Preparation of To prepare the RNase A enzyme for the Thermo Scientific the RNase A KingFisher Plant DNA Kit (Cat. No. 97050196), add 1.25 ml of DNase-free water to the RNase A vial. - Page 20 Storage Conditions and Preparation of the Working Solution Preparation of the RNase A working solution Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 21: Protocols And Pipetting Instructions
With dried material, use only one fifth of the starting material compared to the fresh sample material. For example, instead of using 30 mg of fresh plant leaf, use only 6 mg of dried plant leaf. Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual... -
Page 22: Homogenization Of Sample Material
To clear the plant lysate, centrifuge the samples for 20 minutes (6000 x g). Transfer the supernatant to a Thermo Scientific Microtiter deep well 96 plate and begin the purification of DNA using the KingFisher Flex or KingFisher Duo. See the detailed instructions below. -
Page 23: Instructions For Kingfisher Flex With 96 Deep Well Plates For Dna Purification From 400 Μl Of Plant Lysate
Instructions for These instructions are for the DNA purification from KingFisher Flex 400 µl of plant lysate using the KingFisher Plant DNA Kit (Cat. No. 97050196, 1 x 96) and the KingFisher Flex with with 96 deep well 96 deep well plates. - Page 24 Protocols and Pipetting Instructions Homogenization of sample material 4. Place a Thermo Scientific KingFisher Flex 96 tip comb for deep well magnets on a Tip Plate (that is, an empty KingFisher Flex 96 KF plate). 5. Start the KF_PlantDNA_Flex96 protocol with the KingFisher Flex 96 and load the plates.
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Page 25: Summary Of Plate Contents
96 plate Microtiter Wash 3 Wash Buffer 3 600 µl deep well 96 plate KingFisher Elution Elution Buffer 150 µl Flex 96 KF plate KingFisher Tip Plate Flex 96 KF plate Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual... -
Page 26: 400 Μl Of Plant Lysate
Empty Empty Empty Wash 1 Wash Buffer 1 600 µl Wash 2 Wash Buffer 2 600 µl Wash_EtOH 80% ethanol 600 µl Wash 3 Wash Buffer 3 600 µl Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific... - Page 27 7. After the run is completed, remove the plate and store the purified DNA. When the protocol is completed, remove the plate and elution strip according to the instructions on the Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual...
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Page 28: Summary Of Plate And Elution Strip Contents
Wash Buffer 1 600 µl Wash 2 Wash Buffer 2 600 µl Wash_EtOH 80% ethanol 600 µl Wash 3 Wash Buffer 3 600 µl Elution strip Elution Elution Buffer 100 µl Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific... -
Page 29: Quantification And Determination Of The Purity Of Dna
The ratio between the A260/A280 indicates the purity of the DNA, and the value for DNA should be ≥ 1.7–2.0. A Thermo Scientific NanoDrop can be used for the direct measurement of DNA without diluting the sample. It is recommended that A320 correction is used for the absorbance values. - Page 30 Protocols and Pipetting Instructions Quantification and determination of the purity of DNA Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 31: Chapter 6 General Information
Wash Buffer 3 in order to minimize the loss of DNA during the wash step. A short wash and a slow mixing speed without releasing the magnetic beads into the buffer are recommended. Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual... -
Page 32: Elution Step
BindIt Software 3.2 and according to the volume ranges suitable for the KingFisher instrument. The table below indicates the available elution volumes of the KingFisher instruments. Table 6-1. Available elution volumes of Thermo Scientific KingFisher instruments KingFisher instrument Elution volume KingFisher 20–200 µl... -
Page 33: Thermo Fisher Scientific Thermo Scientific Kingfisher Plant Dna Kit Instruction Manual
RNase treatment is recommended if the purity of the DNA is not sufficient for downstream applications with the normal protocol. Thermo Fisher Scientific Thermo Scientific KingFisher Plant DNA Kit Instruction Manual... - Page 34 General Information Elution step Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 35: Troubleshooting
If the KingFisher instrument mixes too strongly, it may cause partial elution of DNA already in the Wash Buffer 3. Use only Thermo Scientific KingFisher plates, tubes and tip combs with the KingFisher instruments. Use of products from other manufacturers may cause unsuitable mixing and affect the yield of purified DNA. - Page 36 DNA has already been released from the KingFisher Magnetic Beads into the Elution Buffer. If the KingFisher magnetic particle processor does not work properly, refer to the relevant user manual of the KingFisher instrument in use. Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
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Page 37: Ordering Information
Appendix B Ordering Information Table B-1. Thermo Scientific KingFisher Plant DNA Kit Cat. No. Product Package size 97050196 KingFisher Plant DNA Kit 1 x 96 Table B-2. Thermo Scientific KingFisher Flex consumables Cat. No. Product Package size 97002514 KingFisher Flex 96 tip comb for PCR magnet... - Page 38 50 pcs 97002090 KingFisher plastics 100 µl 8-pack, 8 plates and 8 tip combs 1 box 97002094 KingFisher plastics 200 µl 8-pack, 8 plates and 8 tip combs 1 box Thermo Scientific KingFisher Plant DNA Kit Instruction Manual Thermo Fisher Scientific...
- Page 40 Thermo Fisher Scientific Oy Ratastie 2, P.O. Box 100 FI-01621 Vantaa Finland www.thermoscientific.com N11996...
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