Thermo Scientific KingFisher Duo User Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Laboratory Equipment
  5. KingFisher Duo
  6. User manual

Thermo Scientific KingFisher Duo User Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Thermo Scientific
KingFisher Duo
User Manual
Rev. 1.0

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the KingFisher Duo and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Thermo Scientific KingFisher Duo

Summary of Contents for Thermo Scientific KingFisher Duo

  • Page 1 Thermo Scientific KingFisher Duo User Manual Rev. 1.0...
  • Page 3 Thermo Scientific KingFisher Duo User Manual Rev. 1.0, Cat. no. N12420...
  • Page 4 US 6447729, Method and means for magnetic particle specific binding assay, and US 6448092, Separation device for microparticles involving a magnetic rod. KingFisher Duo also has national and international patents pending. Trademarks “BindIt” is a trademark and “KingFisher” and “Microtiter” are registered trademarks of Thermo Fisher Scientific Inc.

  • Page 5: Intended Users

    Thermo Scientific KingFisher Duo magnetic particle processor, including the installation and operating instructions. How to use this This user manual is for the KingFisher Duo instrument (Cat. no. 5400100). It aims to give you the information you need for: user manual Reviewing safety precautions ●...

  • Page 6: Safety Symbols And Markings

    Date of manufacture ▲ Consult instructions for use ▲ WEEE symbol This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. ▲ 4 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 7: Warning And Other Markings Used In The Documentation

    Caution Risk of damage to the instrument, other equipment or loss of performance or function in a specific application. ▲ Note Marks a hint, important information that is useful in the optimum operation of the system, or an item of interest. ▲ Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...

  • Page 8: Instrument Safety And Guidelines For Use

    The device shall be operated only with software specifically designed ● for the device. Never open any other covers of the KingFisher Duo than the front ● lid or the maintenance door (Figure 2–3) while the instrument is plugged into a power source.

  • Page 9: Table Of Contents

    How to use this user manual ............. 3 For more information ............... 3 Safety symbols and markings............. 4 Safety symbols and markings used on the KingFisher Duo..... 4 Warning and other markings used in the documentation ....5 Instrument safety and guidelines for use..........6 Chapter 1 ®...
  • Page 10 Control panel.................. 33 Keypad..................33 Keys ..................33 Display..................34 Navigating ................... 35 Using KingFisher Duo PC software ..........38 Using internal software..............38 Factory / User protocols ..............38 Selecting the protocol..............38 Run menu ..................39 Instrument options................43 Export device report ..............
  • Page 11 List of accessories and consumables ..........69 List of spare parts ................70 List of KingFisher Kits ..............70 Appendix A Certificate of Decontamination..............71 Appendix B System Log ....................73 Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 12 Contents Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 13: Introduction To The Kingfisher ® Duo

    The patented technology of the KingFisher Duo system is based on the use of magnetic rods covered with a disposable, specially designed tip comb and plates. The instrument functions without any dispensing or aspiration parts or devices.
  • Page 14 Introduction to the KingFisher® Duo Principle of operation Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 15: Chapter 2 Functional Description

    Interchangeable KingFisher Duo head Interchangeable heating block Maintenance door (open, dashed line) Turntable Elution block (fixed) Figure 2–2. KingFisher Duo front view with front lid and maintenance door open Display Maintenance door (closed, dashed line) Shield plate (blue dashed line)

  • Page 16: Back / Internal View

    Warning LEDs Figure 2–5. KingFisher Duo internal view the from front processing position KingFisher Duo The KingFisher Duo (Figure 2–6) has room for two plates and two elution strips. The tip combs are compatible with the plates. During the magnetic...

  • Page 17: Principle Of Magnetic Particle Processing

    Magnetic particles are transferred with the aid of magnetic rods covered with a disposable, specially designed plastic tip comb. Figure 2–6. KingFisher Duo magnetic particle processor Principle of magnetic particle processing Figure 2–7.

  • Page 18: Working With A Magnetic Rod

    The volume of the first row of the plate can be larger than the volume of the next row, and this is used for concentration purposes (see during the magnetic Figure 2–8 below). particle processing Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 19: Magnet Heads

    Figure 2–8. A concentration step during magnetic particle processing Magnet heads The KingFisher Duo can be used with one processing head at a time. The instrument comes equipped with two interchangeable KingFisher Duo magnet heads, a 12-pin magnet head and a 6-pin magnet head.

  • Page 20: Elution Blocks

    There are two parallel fixed 1 x 12-well elution blocks (Figure 2–2 and Figure 2–5) with a heating and cooling option. The elution blocks can only be used with a 12-pin magnet head. A plastic KingFisher Duo 1 x 12 elution strip can be placed onto the block.

  • Page 21: Reagents

    For more details on kits available, visit http://www.piercenet.com The KingFisher Duo is an open system, which gives an opportunity to use also other magnetic particle kits. The optimal magnetic particle size for the KingFisher Duo is 0.8–10 μm.
  • Page 22 Functional Description Reagents Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 23: Chapter 3 Installation

    ▲ Warning The KingFisher Duo weighs approximately 16 kg [35 lbs.] without the transport package and should be lifted with care. It is recommended that two persons lift the instrument together, taking the proper precautions to avoid injury.

  • Page 24: Checking Delivery For Completeness And Damage

    Fisher Scientific representative or Thermo Fisher Scientific Oy. Environmental When you set up your KingFisher Duo, avoid sites of operation with excess dust, vibrations, strong magnetic fields, direct sunlight or UV requirements light, draft, excessive moisture or large temperature fluctuations. Place the instrument on a normal laboratory bench.

  • Page 25: Installation Setups

    KingFisher Duo heads. Do not hold the KingFisher Duo heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Duo heads.

  • Page 26: Connecting The Power Supply Cable

    Warning Ensure that the mains switch (Figure 2–4) on the front panel is in the OFF position. Never operate your instrument from a power outlet that has no ground connection. Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region. ▲...

  • Page 27: Operational Check

    Run the check protocol (Check_12-tip or Check_6-tip) under the Maintenance menu according to the KingFisher Duo head and plastics you are using. Ensure that the correct magnet head is attached. If the check is all right, proceed with your own runs.

  • Page 28: Interchangeable Heating Blocks

    Table 3–1. Plates vs. heating adapter(s) Plate Description Heating adapter(s) Description KingFisher Flex 24 deep well KingFisher Duo heating block for 24 plate (200–5000 μl*) DW plate Microtiter deep well 96 plate KingFisher Duo heating block for 96 (50–1000 μl*)

  • Page 29: Interchangeable Kingfisher Duo Magnet Heads

    Keep the KingFisher Duo heads always in their respective plastic boxes when not in use (Figure 3–13). It is very important to keep the KingFisher Duo heads away from each other and other magnets at all times. Clashing of the magnets together may cause serious damage to the magnets.

  • Page 30: Changing The Magnet Head

    KingFisher Duo heads. Do not hold the KingFisher Duo heads close to a PC display, since this may cause damage to the display.
  • Page 31 4. Take away the magnet head by turning the two finger screws on top of the magnet head clockwise when instructed (Figure 3–15). When you remove the KingFisher Duo head, be careful not to damage the magnet rods against the tip comb holder frame.

  • Page 32: Packing The Instrument For Transportation

    Installation Packing the instrument for transportation Figure 3–17. KingFisher Duo 6-pin head for KingFisher Flex 24 deep well plate fitted Packing the This section describes how to refit the transport lock when packing the instrument for transportation or service. instrument for...
  • Page 33 Packing the instrument for transportation Figure 3–18. Refitting the transport lock 5. Figure 3–19 shows the transport lock refitted. ▲ Figure 3–19. Transport lock refitted Caution Ensure that the power is switched off. ▲ Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 34 Installation Packing the instrument for transportation Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 35: Chapter 4 Routine Operation

    Chapter 4 Routine Operation Switching on Before you switch on the KingFisher Duo, ensure that the voltage on the type label at the bottom left of the back panel (Figure 2–4) corresponds to the local voltage. Warning Never operate your instrument from a power outlet that has no ground connection.

  • Page 36: Display

    Icon Last used protocol(s) Info text bar Figure 4–21. Main view on the display of the KingFisher Duo There are three menus in the KingFisher Duo user interface: Protocols, Run and Maintenance. In routine use you mainly navigate in the Protocols/Run menu.

  • Page 37: Navigating

    The info text bar shows explanatory information on how to proceed and which keys to use. Navigating This section visualizes navigation in the KingFisher Duo user interface. The main view changes according to the selections you make either with the Up or Down arrow keys or the button.
  • Page 38 Routine Operation Control panel The main views of each menu tab are shown below. Protocols Maintenance Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...
  • Page 39 “Import protocol” on page 45 “Export protocol” on page 46 “Maintenance protocol” on page 47 “Information” on page 47 “Language” on page 48 “Buzzer” on page 49 “Date and time” on page 49 Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...

  • Page 40: Using Kingfisher Duo Pc Software

    BindIt Software. Duo PC software In addition to the KingFisher Duo internal software features, you can download protocols to the instrument or back up protocols from one instrument and transfer them to another.

  • Page 41: Run Menu

    The time shown refers to when this protocol was last started. Used plates and strips, and when the protocol was last run are also shown. Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 42 Note that whenever a user pauses the operation by pressing the PAUSE button during a run, the Pause icon flashes on the menu bar after the Pause message appears on the display. Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...
  • Page 43 During the pause, the user must be prepared, for example, to load the plate, dispense, or unload the plate and elution strip. Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 44 When a protocol is started in the Protocol menu, a small Run icon is superimposed on the protocol symbol of the active protocol. The views of a one-protocol and a two-protocol run are shown below. Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 45: Instrument Options

    (Figure 4–22 B). Figure 4–22. USB memory device unplugged (A) and plugged in (B) The KingFisher Duo device report includes the serial number, software versions, motor offsets and accelerations, temperature control parameters, the startup status, statistics, the run user, factory and maintenance protocols, error logs, settings as well as miscellaneous information.

  • Page 46: Export Run Log

    You can export the run log. A USB memory device has to be plugged in to the instrument (Figure 4–22 B). The KingFisher Duo run log includes the protocol name, when the protocol was started and stopped, and the plates and strips used of the all the user and factory protocol runs as well as those of all the UV protocol and maintenance protocol runs.

  • Page 47: Import Protocol

    (Figure 4–22 B). Go to the Maintenance menu. Select the Import Protocol row and press Attach the USB memory device or press the Left arrow key to cancel. Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...

  • Page 48: Export Protocol

    Go to the Maintenance menu. Select the Export Protocol row and press Use the Up and Down arrow keys to select the protocol group. Press to continue. Attach the USB memory device or press the Left arrow key to cancel. Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 49: Maintenance Protocol

    Instrument options Maintenance You can set which protocol is automatically selected as the maintenance protocol when the KingFisher Duo is powered on. protocol The maintenance protocols include, for example, the protocols needed to refit the transport lock, change the magnet head, change the heating block or run the operational check protocol.

  • Page 50: Language

    Use the Up and Down arrow keys to select. Press the Left arrow key to return. Language You can set the language of the internal software. Go to the Maintenance menu. Select the Language selection row and press Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 51: Buzzer

    Press the Left arrow key to return. Date and time You can set the date and time of the instrument. Go to the Maintenance menu. Select the Date and Time row and press Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 52 Use the Up and Down arrow keys to select the desired date format. The available date formats are: dd.mm.yyyy, dd/mm/yyyy, dd-mm- yyyy, mm/dd/yyyy, and yyyy-mm-dd. The default is dd.mm.yyyy. Press the Left arrow key to cancel or return. Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 53: Handling The Consumables

    Routine Operation Handling the consumables Handling the How to handle the KingFisher Duo consumables, such as tip combs, plates and elution strip, are described below. For more information, consumables refer to Chapter 8: “Ordering Information”. Tip combs Specially designed tip combs that protect the magnets during the process are available for different plate types and applications.

  • Page 54: Plates

    Routine Operation Handling the consumables Figure 4–25. KingFisher Duo 6-tip comb package with four tip combs 2. Place one of the tip combs onto the tip row (default row B) of an empty KingFisher Flex 24 deep well plate (Figure 4–26).

  • Page 55: Elution Strip

    30–130 μl 96 DW plate Elution strip The KingFisher Duo elution strip can only be used with a KingFisher Duo instrument installed with a 12-pin magnet head (Figure 4–28). Figure 4–28. KingFisher Duo elution strips, separate and in a frame The elution strip is typically used to perform the elution step that concentrates the purified sample.
  • Page 56 The two plate positions and the A1 positions of the two plate positions are clearly marked on the turntable. When the instrument is in its basic position, plate position 1 is under the KingFisher Duo head. Note that at the end of a protocol run, the turntable may stop in a different position than the starting position.
  • Page 57 6. Close the front lid. The front lid protects the instrument against environmental contamination. Note The front lid can be left open if desired. This action does not break the run. ▲ Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...

  • Page 58: Shutdown

    Shutdown To shut down the KingFisher Duo: 1. Switch the KingFisher Duo off by pressing the power switch (Figure 2–3) on the front panel of the instrument into the OFF position. It is recommended to shut down the instrument for the night and weekends.

  • Page 59: Chapter 5 Maintenance

    Although the KingFisher Duo is constructed from high-quality materials, you must immediately wipe away spilled saline solutions, solvents, acids or alkaline solutions from outer surfaces to prevent damage.

  • Page 60: Cleaning The Magnetic Rods

    KingFisher Duo heads. Do not hold the KingFisher Duo heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Duo heads.
  • Page 61 7. Place a cloth soaked in the prepared solution into the bag. Ensure that the cloth does not come into contact with the instrument. 8. Seal the bag and leave the instrument in the bag for at least 24 hours. Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...

  • Page 62: Packing For Service

    Decontaminate the instrument beforehand. ● Install the transport lock. ● Place the KingFisher Duo head into its transport package. ● Pack the instrument according to the enclosed packing instructions. ● Use the original packaging to ensure that no damage will occur to ●...

  • Page 63: Service Contracts

    B: “System Log”. Copy the table as many times as necessary, but leave the blank original inside the user manual. Disposal of the If the KingFisher Duo has to be disposed of, follow the guidelines below. instrument Warning Decontaminate the instrument before disposal. Refer to “Decontamination procedure”...
  • Page 64 Maintenance Disposal of the instrument Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 65: Chapter 6 Technical Specifications

    Protocol import Using PC or USB memory device BindIt Software compatibility Computer interface Robot compatibility Normal use 10 runs/day, 250 days/year, with 40 min protocols having a medium speed setting, RT Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...

  • Page 66: Performance Specifications

    PAUSE STOP ROTATE BACK (Left arrow key) / LCD 5” 480 x 800 pixel color display Safety This section describes the safety specifications for the KingFisher Duo instrument. specifications In conformity KingFisher Duo bears the following markings: with the Type 706...
  • Page 67 Technical Specifications In conformity with the requirements KingFisher Duo conforms to the following requirements: 2006/95/EC (Low Voltage Directive) 2004/108/EC (Electromagnetic Compatibility Directive, EMC) FCC Part 15, Subpart B/Class B (July 2004) 2002/96/EC (Waste of Electrical and Electronic Equipment) 2002/95/EC (RoHS Directive – Restriction of the Use of Certain Hazardous Substances in...
  • Page 68 Technical Specifications In conformity with the requirements Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 69: Chapter 7 Troubleshooting Guide

    The plastic tip comb is not attached to the holder. Check if the tips are present. If it looks all right, turn ON and OFF, and run the check protocol according to the KingFisher Duo head and plastics you are using. 12, 21, 23–24 Error in temperature control.
  • Page 70 Protocol aborted by user. No action needed. Information only. Clock battery is empty. Contact authorized technical service. Aborted because there was an error in another No action needed. Information only. protocol. Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 71: Chapter 8 Ordering Information

    Contact your local Thermo Fisher Scientific representative for ordering and service information. Ordering information codes are presented in Table 8–8 through Table 8–10. KingFisher Duo The following configurations of the KingFisher Duo system are available (Table 8–8). Table 8–8. Codes for products Code...

  • Page 72: List Of Spare Parts

    Ordering Information List of spare parts List of spare The following spare parts are to be used with the KingFisher Duo instrument (Table 8–10). parts Table 8–10. Codes for spare parts Code Item Quantity N12459 KingFisher Duo head for 96 DW...

  • Page 73: Appendix A Certificate Of Decontamination

    *) The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials. This unit is certified by the undersigned to be free of radioactive contamination. Date and place: Signature: Name (block capitals): PHOTOCOPIABLE Please include decontaminating solution used. Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 74 Certificate of Decontamination Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...

  • Page 75: Appendix B System Log

    Appendix B System Log Instrument name/number: User Date Comments PHOTOCOPIABLE Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 76 System Log Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...
  • Page 77 Figure 4–25): Microtiter deep well 96 plates (Table 3–1). • for KingFisher Flex 24 deep well plates KingFisher Duo head There are two kinds of • for Microtiter deep well 96 plates interchangeable KingFisher Duo heads available, for KingFisher Flex 24 deep well...
  • Page 78 Glossary: 76 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...
  • Page 79 49 installation, 3, 21, 22, 23, 25, 60, 65 decontaminating the instrument, 56, 58, 61, 71 installing the KingFisher Duo, 21, 23, 65 decontamination, 56, 58, 59, 60, 61, 71 instrument layout, 13 decontamination procedure, 56, 58, 59, 60, 61, 71...
  • Page 80 4, 5, 6, 21, 23, 24, 26, 28, 33, 56, 57, 58, 59, 61 refitting the transport lock, 24, 30, 31 working with a magnetic rod, 16 releasing the transport lock, 23, 24 RNA, 19, 37, 70 78 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...
  • Page 81 Notes Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual...
  • Page 82 Notes 80 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific...
  • Page 84 Thermo Fisher Scientific Oy Ratastie 2, P.O. Box 100 FI-01621 Vantaa Finland www.thermoscientific.com N12420...

Table of Contents