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Related Manuals for Thermo Scientific KingFisher Presto
Summary of Contents for Thermo Scientific KingFisher Presto
- Page 1 Thermo Scientific KingFisher Presto User Manual Rev. 1.0, Cat. No. N17413...
- Page 2 Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Release history: September 2016: First release for KingFisher Presto For Research Use Only. Not for use in diagnostic procedures.
- Page 3 Warranty statement Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against defective parts and materials, including defects caused by poor workmanship, for a period of one year from the date of delivery. Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific’s instructions.
- Page 5 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
- Page 6 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
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Page 7: Preface
• Perform basic cleaning and maintenance procedures • Troubleshoot the instrument performance This manual also describes the features and specifications of the KingFisher Presto instrument as well as ordering information. Read the manual in its entirety before operating the instrument. -
Page 8: Safety Symbols And Markings On Kingfisher Presto
Note Marks a hint, important information that is useful for the optimum operation of the system, or an item of interest. Tip Highlights helpful information that can make a task easier. Safety Symbols and Markings on KingFisher Presto The following symbols and markings appear on the type label and the instrument itself: WARNING Biohazard risk. -
Page 9: Instrument Safety And Guidelines For Use
• Always follow basic safety precautions and local safety guidelines when using KingFisher Presto to reduce the risk of injury, biohazardous contamination, fire, or electric shock. - Page 10 Preface Contacting Us Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific...
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Page 11: Table Of Contents
Display and Keypad..........4 KingFisher Presto Purification System ....... . . 5 Principle of Magnetic Particle Processing. - Page 12 Install KingFisher Presto ........
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Page 13: Introduction To Kingfisher Presto
Figure 1. KingFisher Presto purification system Operation Principle KingFisher Presto is intended to be used as a part of an automation system. By itself KingFisher Presto can only be used to perform maintenance operations. Thermo Fisher Scientific Thermo Scientific KingFisher Duo PrimeTechnical Manual... - Page 14 Introduction to KingFisher Presto The patented technology of the KingFisher Presto system is based on the use of magnetic rods covered with a disposable, specially designed tip comb and plates. The instrument functions without any dispensing or aspiration parts or devices.
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Page 15: Functional Description
Functional Description Instrument Layout This section shows the front, side and back views of KingFisher Presto. Front View The front view of the KingFisher Presto instrument and its parts. Figure 2. KingFisher Presto front view Item number Description Item number... -
Page 16: Side And Back View
Functional Description Display and Keypad Side and Back View The side and back view of KingFisher Presto: Figure 3. KingFisher Presto side and back view Item number Description Item number Description Ethernet port Power supply cable connector USB port Cooling air inlet... -
Page 17: Kingfisher Presto Purification System
Use the Rotate button to rotate the turntable. KingFisher Presto Purification System The KingFisher Presto purification system has two slots for plates. One is for processing and the other for loading and unloading plates. Disposable tip combs protect the magnet rods on the magnet head during processing and are compatible with the plates. -
Page 18: Principle Of Magnetic Particle Processing
BindIt Software, you need to load the plates manually. If you are using KingFisher Presto as a part of an automation system, a gripper or a robotic arm automatically loads the plate. The plate is placed onto the turntable according to the protocol instructions and the turntable turns the plate below the processing head. -
Page 19: Kingfisher Presto Ui
KingFisher Presto has an internal user interface (UI) with which you can perform maintenance operations only. To execute any other protocols, KingFisher Presto must be connected to a PC equipped with Thermo Scientific BindIt Software or a similar automation software. -
Page 20: Protocols
The magnet head you are using dictates the heating block and consumables that you can use. For more information on matching parts, see Table The KingFisher Presto 24 DW magnet head is used with a corresponding plastic 24 DW tip comb and a KingFisher 24 DW plate. • KingFisher Presto 24 DW magnet head •... -
Page 21: Heating Blocks
Functional Description Heating Blocks The KingFisher Presto 96 DW magnet head is used with a corresponding plastic 96 DW tip comb and either a KingFisher 96 DW plate or a KingFisher 96 plate. • KingFisher Presto 96 DW magnet head •... -
Page 22: Plate Detector
Specially designed tip combs protect the magnet rods during the runs. There are two types of tip combs available for different plate types and applications: • Use the KingFisher 96 DW tip comb with the KingFisher Presto 96 DW magnet head • Use the KingFisher 24 DW tip comb with the KingFisher Presto 24 DW magnet head For more details and ordering information on plastic consumables, see “Handle Tip Combs and Plates”... -
Page 23: Plates
Functional Description Consumables Plates KingFisher Presto is compatible with three different kinds of plates. There are two deep well plates, the KingFisher 24 DW plate and the KingFisher 96 DW plate. The third kind is the KingFisher 96 plate. Figure 8. -
Page 24: Matching Parts
Matching Parts The following table show which heating blocks and consumables to use depending on the magnet head. Table 3. Matching magnet heads, plates and consumables KingFisher Presto with 96 DW head KingFisher Presto with 24 DW head Magnet heads... -
Page 25: Reagents
Reagents Reagents KingFisher Presto allows the use of wide range of magnetic particle reagents. The optimal magnetic particle size for KingFisher Presto is 0.8–10 μm. Thermo Scientific offers a versatile reagent selection for purifying DNA, RNA and proteins. For more information, visit www.thermofisher.com/kingfisher... - Page 26 Functional Description Reagents Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific...
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Page 27: Installation
Unpack the KingFisher Presto instrument and accessories carefully with the arrows on the transport package pointing upwards. Remove the instrument from the package and place it on a level surface. -
Page 28: Operating Conditions
• The humidity is low to avoid condensation, (relative humidity is between 10% and 80%). Install KingFisher Presto in a protected location where no one can step on or trip over the power cord, and where the power cord remains accessible if the unit needs to be unplugged. -
Page 29: Install Kingfisher Presto
Presto heads always in their respective storage boxes when not in use. It is very important to keep KingFisher Presto heads away from each other and other magnets at all times. Clashing of the magnets together may cause serious damage to the magnets. - Page 30 CAUTION Make sure the transport locks are released before you start using the instrument. Figure 9. KingFisher Presto with the transport lock finger screws (1) and warning slips (2) fitted To release the transport locks: 1. Unscrew the transport lock finger screws counterclockwise.
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Page 31: Connect The Power Supply Cable
CAUTION Check that the main power switch on the side of the instrument is in the OFF position. Never operate your instrument from a power outlet that has no ground connection. Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region. To connect the power supply cable: 1. -
Page 32: Install The Magnet Head
1. Find the Change heating block protocol by pressing the Select button. 2. Press Start. The tip comb holder rises. 3. Insert the heating block: a. Set the heating block into its slot. Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific... -
Page 33: Operational Check
To perform the operational check: 1. Take a tip comb and a plate that match the magnet head you are using and place the tip com on the plate. Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... -
Page 34: Connect The Instrument To A Pc
If the check is successful, proceed with your own runs. Connect the Instrument to a PC If you are using KingFisher Presto by itself, use the delivered USB cable to connect the instrument to a PC with BindIt Software installed. The software automatically recognizes the instrument. -
Page 35: Operation
Instruments User Manual. For more information on using the instrument as a part of an automation system, refer to Thermo Scientific™ KingFisher™ Presto Integration guide. Before you switch on the KingFisher Presto instrument, make sure that the voltage on the type label at the bottom right of the back panel (see... -
Page 36: View Instrument Data
The software version can also be seen from BindIt Software. For more information, refer to Thermo Scientific™ BindIt™ Software for KingFisher™ Instruments User Manual. Handle Tip Combs and Plates This chapter advises on how to handle the tip combs and plates compatible with KingFisher Presto. For more information, see chapter “Ordering Information”... -
Page 37: Kingfisher 96 Dw Tip Combs And Plates
3. Store the unused tip comb and plate for later use. KingFisher 24 DW Tip Combs and Plates 1. Unpack a KingFisher 24 DW tip comb package where the tip comb is packed onto a KingFisher 24 DW plate. Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... -
Page 38: Run Protocols
Note The protocol does not continue if the plate detector does not detect a suitable plate on the loading station. Note KingFisher Presto has room for two plates only, so you need to change the plates manually in the given order, if your protocol includes more than two plates. -
Page 39: Shutdown
(SDS, sodium dodecyl sulfate) or soap solution. 4. If you have spilled infectious agents on the turntable, disinfect it with 70% ethanol or another disinfectant (see “Decontamination Procedure” page 32). Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... -
Page 40: Emergency Situations
Note Do not disassemble the instrument. 5. If the corrective measures do not help, contact authorized technical service or your local Thermo Fisher Scientific representative. See also chapter “Maintenance” page 29 “Troubleshooting” page Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific... -
Page 41: Chapter 5 Maintenance
Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol. WARNING If any surfaces are contaminated with biohazardous material, apply mild sterilizing solution to the surface. CAUTION Do not autoclave any part of the instrument. Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... -
Page 42: Clean The Turntable
2. Rotate the finger screw counterclockwise until the screw is no longer attached to the body of the instrument. Figure 19. Loosening the screw. The screw remains attached to the turntable. Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific... -
Page 43: Clean The Magnetic Rods
KingFisher Presto heads. Do not hold KingFisher Presto magnet heads close to a PC display, as this may damage the display. Do not use metal tools when handling KingFisher Presto heads. Be careful not to break the magnets while cleaning. -
Page 44: Disposal Of Materials
6. Place a cloth soaked in the prepared solution into the bag. Ensure that the cloth does not come into contact with the instrument. 7. Seal the bag and leave the instrument in the bag for at least 24 hours. Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific... -
Page 45: Pack The Instrument For Transportation Or Service
• Inform the service about the use of hazardous materials. • Decontaminate the instrument beforehand. • Remove the KingFisher Presto magnet head by using the Change magnet head protocol and place it into its transport package. • Remove the heating block by using the Change heating block protocol. - Page 46 Press Start. c. Gently pull the magnet head until the pins holding the head snap out of their notches. CAUTION When you remove the KingFisher Presto head, be careful not to damage the magnet rods against the tip comb holder frame.
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Page 47: Service Contracts
For more information, contact the Thermo Fisher Scientific technical service department. Disposal of Instrument If the KingFisher Presto has to be disposed of, follow the guidelines given in this section. WARNING Decontaminate the instrument before disposal. For more information, see “Decontamination Procedure”... - Page 48 Method of disposal Electronic waste Contaminated waste (Infectious waste) Regarding the original packaging and packing materials, use the recycling operators known to you. For more information, contact your local Thermo Fisher Scientific representative. Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific...
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Page 49: Chapter 6 Technical Specifications
175 VA max., 55 VA standby Heat dissipation 398 BTU max. Protocol import Using Thermo Scientific™ BindIt™ Software for KingFisher™ instruments BindIt Software compatibility Computer port USB, serial RS-232 or Ethernet Robot compatibility Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... -
Page 50: Performance Specifications
SELECT / START / STOP / ROTATE 2x16 character display Safety Specifications This section describes the safety specifications for the KingFisher Presto instrument which are in conformity with the requirements. In conformity with the requirements, KingFisher Presto bears the following markings: •... - Page 51 Note 2: The pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally, only non-conductive pollution, such as dust, occurs with the exception of occasional conductivity caused by condensation. Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual...
- Page 52 Technical Specifications Safety Specifications Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific...
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Page 53: Chapter 7 Troubleshooting
When an error is detected, terminate the current operation. After an error occurred, abort the current run and restart from the beginning when the problem is fixed. The following table provides a troubleshooting guide for the KingFisher Presto instrument. Error codes Table 8. - Page 54 OFF and ON position and run the check protocol according to the KingFisher Presto head and the tip comb and plate you are using. Thermo Scientific KingFisher Presto User Manual...
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Page 55: Warning Codes
Protocol execution was aborted by the user No actions needed. Step execution was aborted by the user No actions needed. Existing protocol was overwritten There was a protocol with the same name. No actions needed. Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... - Page 56 Warning message Action code Heater preheat was turned off between No actions needed. single step executions Heater was turned off after 10 minutes No actions needed. since last single step execution Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific...
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Page 57: Chapter 8 Ordering Information
5400830 KingFisher Presto with 96 DW head 5400840 KingFisher Presto with 24 DW head Accessories and Consumables The following accessories and consumables are to be used with KingFisher Presto: Table 11. Codes for accessories and consumables Code Item Quantity (pieces) - Page 58 Ordering Information Accessories and Consumables Table 11. Codes for accessories and consumables Code Item Quantity (pieces) 24078841 KingFisher Presto 24 DW head with 1 heating block 24078830 KingFisher Presto 96 DW head 24078840 KingFisher Presto 24 DW head 24075420 96 KingFisher heating block for KingFisher...
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Page 59: Chapter A
*) The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials. This unit is certified by the undersigned to be free of radioactive contamination. Date and place: ___________________________________________________________ Please include decontamination solution used. Thermo Fisher Scientific Thermo Scientific KingFisher Presto User Manual... - Page 60 Appendix A Certificate of Decontamination Signature: _______________________________________________________________ Name (block capitals): ______________________________________________________ PHOTOCOPIABLE Thermo Scientific KingFisher Presto User Manual Thermo Fisher Scientific...