Important Differences Between Spectronic 200 And Spectronic 20D; Experimental Modes - Thermo Scientific SPECTRONIC 200 User Manual

SPECTRONIC 200E USER GUIDE
SPEC 20 D+ Emulation

Important Differences Between SPECTRONIC 200 and SPECTRONIC 20D+

Experimental Modes

Transmittance and Absorbance
26 SPECTRONIC 200E User Guide
The application modes are exactly those found in the SPECTRONIC20D+. Change mode by
pressing the right arrow key as indicated on the screen
wavelength. This knob has a coarse/fine feature. Turn it normally to alter the wavelength in
units of 10 nm. Press it down and turn to alter the wavelength in units of 1 nm.
Note To perform an OD600 measurement for measurement of a cell culture with data
that emulates the results from a SPECTRONIC 20 you should utilize the OD600 mode
accessed from the Home menu. See
details.
SPECTRONIC 200 performs a full spectrum 0%T measurement at startup and retains this
information in internal memory. Similarly, when a user places a blank cuvette in the sample
0.00
stage and presses
spectrum and records the 100%T value at all wavelengths. This means that it is never
necessary for the user to manually set 0%T, and after measuring 100%T at one wavelength, it
is not necessary to measure it again if the user changes measurement wavelength.
Simply place a blank solution in the sample stage and press
(100%T) measurement completes, place your sample cuvette in the sample stage and the
instrument will display the transmittance or absorbance, as selected
on the navigation pad to toggle between Absorbance and Transmittance modes.
Figure 41.
OD600 Mode for Cell Growth Measurements
on the instrument keypad, the SPECTRONIC 200 measures the
41). Use the λ knob to set the
(Figure
0.00
. Once the Auto Zero
(Figure 41). Press
for
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