Chromeleon; Control; Overview - LC Packings SWITCHOS II User Manual

CHAPTER 4
4.1
This chapter provides information about how to setup the CHROMELEON
Software to control the Switchos II Advanced Microcolumn Switching Unit either
by the RS-232 communication port or by the event outputs of the Switchos II
loading pump. A detailed description of the software features is provided in
CHROMELEON online help (F1 key) and a quick "Getting Started" reference can
be found in the UltiMate User's Manual. It is assumed that the user has a basic
understanding of the CHROMELEON software and its modules.
When the Switchos™ II Advanced Microcolumn Switching Unit is used in
conjunction with the LC Packings UltiMate™ Capillary HPLC System and the
CHROMELEON software, the flow rate of the loading pump as well as the
position of the valves of the Switchos II can be programmed from the
CHROMELEON software. The Switchos II has to be installed as described in
Chapter 2.
The role of the valves of the Switchos II is dependent on the application for
which the unit is configured (e.g. if the FAMOS Microautosampler is included in
the system). The user should ensure that the electrical interface and the fluidic
interface to other devices in the system meet the requirements of the analysis.
Note: All dialog boxes and information refer to CHROMELEON 6.5 SP3.
In this discussion, we will describe how to establish a typical column switching
application with the following parameters:
• Sample load/clean-up time:
• Gradient analysis time:
User's Manual Switchos II

CHROMELEON

Overview

D850R3
®

Control

5 min (at a loading flow rate of 30µL/min)
50 min
®
4-1