Setting Up The Fluidics Connections - LC Packings SWITCHOS II User Manual

Installation and Getting Started
i) Set the purge flow to 0.5 mL/min. Allow the system to purge for at least 3
min. After line A has been purged, repeat the process for all other lines.
j) Close the purge valve on the loading pump by turning the purge valve knob
clockwise.
k) Close the He shut-off valve(s) on top of the solvent bottles that will not be
used (the white line should be horizontal).
l) Place the loading pump under computer control and deliver mobile phase
through the entire HPLC system at the flow rate and from the solvent
reservoir that are used for the initial conditions for the analysis that you
intend to perform. As the system is delivering mobile phase, check for leaks,
monitor the baseline and check that the pressure is similar to what was
observed when the system was last used.
2.6.4 Setting-up the Fluidic Connections
The Switchos is used in a broad range of applications and the user can configure
the unit to meet the specific needs of the laboratory. In this section we show the
fluidic setup of a pre-concentration application using the Switchos Advanced
Microcolumn Switching Unit in conjunction with the FAMOS Microautosampler
and the UltiMate Capillary HPLC System (FIGURE 2-12). Additional examples are
presented in Appendix C. While these examples may not meet the specific needs
of the analyst, it is likely that they can be used with minor modification.
Pre-Concentration using the Switchos and the FAMOS Microautosampler
FAMOS
port 6
FAMOS
port 1
FIGURE 2-12 Typical Pre-Concentration Setup (Loading the Trap Column)
To setup the Switchos for the pre-concentration application presented in FIGURE
2-12, connect the instruments as follows:
a) Connect port 1 of the injection valve of the FAMOS Microautosampler to the
Nano/Micro flow outlet of the Switchos loading pump using the 130 µ m I.D.
PEEK tubing provided with the instrument:
2-12
Switchos
Valve A
Trap column
D850R3
Analytical microcolumn
Micro flow
to UltiMate
Waste
1
10
2
9
3 Valve A
presented
in position 8
4
1-2
5
7
6
Micro flow
from UltiMate
Waste
User's Manual Switchos