Introduction; Features Of The Switchos ™ Ii Advanced Microcolumn; Switching Unit - LC Packings SWITCHOS II User Manual

CHAPTER 1
1.1 Features of the Switchos
The Switchos™ II Advanced Microcolumn Switching Unit is an advanced
switching system for use with micro-HPLC systems. The system incorporates the
following features:
• Two Valco 10-port low-dispersion switching valves which allow for the
connection of capillary, micro and nano HPLC columns without any dead
volume.
• A high precision loading pump, fully controllable by the CHROMELEON
software.
• Four channel solvent selection valve.
• Automatic control of all valves by the CHROMELEON software and manual
control via push buttons.
• Manual valve switching is possible at any time, even when a program is
running.
• LED's which indicate the present status of the switching valves (microfluidic
pathways), the position of the solvent selection valve and the control mode.
A broad range of applications can be performed using micro-column switching
with the Switchos II including sample preconcentration, sample cleanup, multi-
dimensional separations (2-D separations), desalting and selective extraction
Typical examples of the use of the unit (which are described in Section 2.6.4 and
Appendix C) include immuno-affinity extractions, isolation of phosphorylated
peptides from complex protein digests, automated removal of detergents,
extraction of drugs from biological fluids, high throughput analysis, etc.
Switchos II is configured with an LC Packings UltiMate™ Micropump and is
normally used with the LC Packings UltiMate Capillary HPLC System. The system
is fully compatible with other instrumentation (a minimum of three contact
closures, TTL or Open Collector terminals are required).
User's Manual Switchos II

Introduction

Switching Unit

™
II Advanced Microcolumn
D850R3
®
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