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LC Packings SWITCHOS II User Manual

Advanced microcolumn switching unit
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Switchos
II
™
Advanced Microcolumn Switching Unit
User's Manual
P/N 162013
www.lcpackings.com
D850R3

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Summary of Contents for LC Packings SWITCHOS II

  • Page 1 Switchos ™ Advanced Microcolumn Switching Unit User’s Manual P/N 162013 www.lcpackings.com D850R3...
  • Page 2 (but not limited to) electrical hazards, chemical solvent hazards and the exposure to pressurized solvents. © October 2004, LC Packings, BV - A Dionex Company. All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of LC Packings, BV.

  • Page 3: Table Of Contents

    Installation and Getting Started CHAPTER 2 Installation 2.1.1 Location of Switchos II in the Laboratory Unpacking Installing the Switchos II with the UltiMate Capillary HPLC System and the FAMOS Microautosampler 2.3.1 Electrical Connections 2.3.2 RS-232 Connectors 2.3.3 COMMUNICATION Connector 2.3.4 INPUTS Connector 2.3.5 Triggering a Mass Spectrometer (or Other Device)
  • Page 4 CHAPTER 4 Overview Server Configuration 4.2.1 Adding and Configuring the Switchos 4.2.2 Switchos II controlled by a RS-232 COM Port 4.2.3 Switchos II controlled by Event Outputs 4.2.4 Adding and Configuring the Virtual Channel Driver UltiMate Event Output Programming 4.3.1 Starting CHROMELEON 4.3.2 The Control Panel...
  • Page 5 Overview Maintenance Disassembly/Reassembly of the Valve A.3.1 Disassembly of the Valve A.3.2 Reassembly of the Valve Network Identification APPENDIX B Identifying the Switchos II on the Network Cleanup of Proteins APPENDIX C Overview Connecting Fused Silica Capillary APPENDIX D Overview...
  • Page 6 Table of Contents [This page intentionally left blank] User’s Manual Switchos II D850R3...

  • Page 7: Warranty

    If any product covered by this warranty becomes defective during the warranty period, it will be repaired or replaced by LC Packings at no charge to the customer (the repair/replace decision is solely at the option of LC Packings). All warranty requests must be received by LC Packings during the warranty period.
  • Page 8 LC Packings has been advised of the possibility of such damages.

  • Page 9: Instructions For Returning Instruments

    A purchase order (if the system is not in warranty) Note: The completed and signed HEALTH AND SAFETY form must be returned to LC Packings service department (fax or mail) prior to the return of any component, or attached outside the shipping package. In addition, the provided RMA number must be clearly marked on the outside of the shipping package.
  • Page 10 Instructions for Returning Instruments [This page intentionally left blank] viii User’s Manual Switchos II D850R3...
  • Page 11 Disconnect the instrument from all power sources before removing the panels. 6) Always replace blown fuses with fuses of the same size and rating indicated on the fuse holder and panel. Refer to Section 6.3.6 of this manual for more information on Fuses. User’s Manual Switchos II D850R3...

  • Page 12: Warnings And Safety Precautions

    (50/50) before it is shut down. This will prevent salt buildup inside the unit. 15) Do not use the instrument in ways other than those indicated in the instructions given in this manual. User’s Manual Switchos II D850R3...

  • Page 13: Ce Declaration Of Conformity

    EN61010-1 Safety requirements for laboratory equipment (Class I, Installation cat. II, Pollution degree II) LC Packings will not accept any liability for damages direct or indirect caused by connecting this instrument to devices which do not meet relevant safety standards.
  • Page 14 CE Declaration [This page intentionally left blank] User’s Manual Switchos II D850R3...

  • Page 15: Introduction

    Switchos II is configured with an LC Packings UltiMate™ Micropump and is normally used with the LC Packings UltiMate Capillary HPLC System. The system is fully compatible with other instrumentation (a minimum of three contact closures, TTL or Open Collector terminals are required).

  • Page 16: General Design Of The Switchos Ii Advanced Microcolumn Switching Unit

    Introduction 1.2 General Design of the Switchos II Advanced Microcolumn Switching Unit A schematic diagram of general design of the Switchos II Advanced Microcolumn Switching Unit is presented in FIGURE 1-1. Solvent lines A - D to the SSV Solvent Bottles...
  • Page 17 Introduction • Loading Pump - designed for the use in the LC Packings UltiMate Capillary HPLC system, the micropump guarantees highest performance and perfect compatibility. It is fully controllable via the CHROMELEON software. • Solvent Bottles and He Degassing System - provides mobile phase to the system.

  • Page 18: Front View Of Switchos

    Introduction Front View of Switchos II The front view of the Switchos II Advanced Microcolumn Switching Unit is presented in FIGURE 1-2. ON/STANDBY Switch He Regulating Valve LEDs Solvent Selection Valve Valve A LEDs Valve A Button with LED Valve A...

  • Page 19: Rear View Of Switchos

    Introduction 1.4 Rear View of Switchos II The rear view of the Switchos II Advanced Microcolumn Switching Unit is presented in FIGURE 1-3. SOLVENT SELECTION Button REMOTE/LOCAL Switch He Inlet COMMUNICATION Connector INPUTS Connector Power Outlet to Loading Pump Main Power Switch...

  • Page 20: Contents Of This Manual

    Introduction 1.5 Contents of this Manual Note: This manual covers the standard version of the Switchos II Advanced Microcolumn Switching Unit as well as the inert version. If you are using an inert version, please refer to Appendix D, which includes important information about how to connect fused silica tubing to the inert switching valves.

  • Page 21: For Additional Information

    CHROMELEON (F1 key). In addition to the ‘Switchos II Advanced Microcolumn Switching Unit - User’s Manual’ this binder contains a copy of the User’s Manual for the LC Packings ™ UltiMate Micropump.
  • Page 22 Introduction [This page intentionally left blank] User’s Manual Switchos II D850R3...

  • Page 23: Installation And Getting Started

    The instructions provided below are provided for installation of the Switchos ™ Advanced Microcolumn Switching Unit as part of the LC Packings UltiMate Capillary HPLC System as well as for installation of the instrument as a stand- alone component in an HPLC system.

  • Page 24: Unpacking

    If there is external damage to the shipping box, the damage should be reported to the shipping agent and LC Packings upon receipt of the goods. If internal damage is observed or if any items are missing, this should be reported to the shipping agent and to LC Packings as soon as it is observed.

  • Page 25: Installing The Switchos Ii With The Ultimate Capillary Hplc

    Installing the Switchos with the UltiMate Capillary HPLC System and the FAMOS Microautosampler When the Switchos is used as a component in the LC Packings UltiMate Capillary HPLC system, all instruments are controlled by the CHROMELEON software. The loading pump will be included in the instrument network and the switching valves are controlled by a COM port of the PC.

  • Page 26: Rs-232 Connectors

    Switchos loading pump and the COM port of the PC. Use a Serial Communication Cable to connect the other RS-232 connectors of both pumps. Caution: The RS-232 sockets are to be used only with LC Packings software CAUTION products (e.g. CHROMELEON) or third vendor software that support the LC Packings UltiMate System.

  • Page 27: Communication Connector

    Installation and Getting Started 2.3.3 COMMUNICATION Connector Connect the COMMUNICATION port to a free COM port on the computer using the Solvent Organizer Com cable (item c, FIGURE 2-2). 2.3.4 INPUTS Connector CHROMELEON controls the valve positions via the COMMUNICATION port (Section 2.3.3).

  • Page 28: Using The Event Outputs Or The Famos Auxiliaries

    Valves are controlled by the Loading Pump Connect the INPUTS connector to the EVENTS outputs of the loading pump using the connection cable P/N 160172 presented in FIGURE 2-4. Switchos Events Micropump Switchos II EVENT3 EVENT3 Valve A GROUND...

  • Page 29: Installing The Switchos With Other Hplc Systems

    (Chapter 7). If you have any questions, please call LC Packings. The LC Packings warranty will not be valid if the Switchos is damaged due to interfacing of the system to instrumentation from third parties.

  • Page 30: Power Connector

    Installation and Getting Started VALVE A(B) - these two pins control the two switching valves. An open contact closure or an inactive TTL (or open collector) output will drive the corresponding valve into position “1-2”, while an active input will drive the corresponding valve into position “10-1”.

  • Page 31: Fluidic Connections - Switchos Ii To The Hplc System

    Installation and Getting Started Fluidic Connections - Switchos to the HPLC System 2.6.1 Preliminary Operations To connect the Switchos Advanced Microcolumn Switching Unit to an HPLC system and to prepare the instrument for operation: • Place the Switchos Advanced Microcolumn Switching Unit in its operating location, preferably on the left side of the HPLC system.

  • Page 32: Sparging The Mobile Phase And Flushing The Solvent Lines

    Installation and Getting Started 2.6.3 Sparging the Mobile Phase and Flushing the Solvent Lines Although the Switchos loading pump is controlled via the CHROMELEON software, initial system preparation is done with the pump on an off-line basis (i.e. the pump is operated locally). To prepare the system for a separation: a) Power up the Switchos and make certain that power is provided to the pump.
  • Page 33 Installation and Getting Started Vent He Shut-Off Valve FIGURE 2-9 He Shut-Off Valve Outlet Solvent Inlet FIGURE 2-10 (A) Rapid Sparging (B) Sparging – Maintenance Mode (Shield removed to provide clarity) Connect the 5 mL syringe to the purge outlet on the purge valve on the loading pump using 1/16”...

  • Page 34: Setting Up The Fluidics Connections

    Installation and Getting Started Set the purge flow to 0.5 mL/min. Allow the system to purge for at least 3 min. After line A has been purged, repeat the process for all other lines. Close the purge valve on the loading pump by turning the purge valve knob clockwise.
  • Page 35 Connect ports 6 and 10 of valve A of Switchos to waste (e.g. using 200 - 500 µm I.D. PTFE tubing). f) Connect the trap column between ports 2 and 5 of valve A of the Switchos II, using the appropriate tubing supplied with the trap column.

  • Page 36: Routine Operation Of The System

    Installation and Getting Started Routine Operation of the System 2.7.1 Sample and Mobile Phase Considerations The Switchos Advanced Microcolumn Switching Unit is used in an HPLC system and the “standard” operating precautions for HPLC should be employed: • Ensure that samples and mobile phases do not contain particulate matter. All samples and mobile phases should be filtered through a 0.22 µm membrane filter.

  • Page 37: The User Interface

    The User Interface CHAPTER 3 Overview This chapter describes the general mode of operation of the Switchos™ Advanced Microcolumn Switching Unit. It includes the following information: • Powering Up the Switchos (Section 3.2) • A description of the controls on the front and rear panel (Section 3.3) •...

  • Page 38: Powering Up The Switchos

    The User Interface Powering up the Switchos When the Switchos Advanced Microcolumn Switching Unit is powered up via the main power switch on the rear panel (FIGURE 2-1), it will go through an initialization/self-test protocol. During this period, all LEDs on the front panel will be illuminated for a short period of time and a number of messages are displayed on loading pump indicating that various components are functioning properly.

  • Page 39: Controls On The Front And Rear Panel

    The User Interface Controls on the Front and Rear Panel When the Switchos Advanced Microcolumn Switching Unit is used on a local basis, the user sets the desired operating conditions via the controls on the front panel and the push button and switch located on the rear panel. The present status of the system is indicated by a series of LEDs on the front panel.

  • Page 40: Local Mode Vs Remote Mode

    The User Interface The upper rear panel of the Switchos (FIGURE 3-4) includes the following: SOLVENT SELECTION Button REMOTE/LOCAL Switch FIGURE 3-4 Control Elements on the Upper Rear Panel • SSV (Solvent Selection Valves) Button – pressing the button will step to the next solvent channel, from A to B, B to C, …...

  • Page 41: Basic Operations For The Loading Pump

    The User Interface Basic Operations for the Loading Pump The main screen of the loading pump includes a series of parameters that are used to set the principal operating conditions for delivering the mobile phase, such as the flow rate and maximum pressure. In automated mode (networking) these parameters are set via CHROMELEON.

  • Page 42: Starting And Stopping Flow Delivery

    The User Interface 3.5.3 Starting and Stopping Flow Delivery When the desired parameters have been entered, the flow delivery can be started with the START/STOP key (item 2, FIGURE 3-5). This key is also used to stop the flow delivery. 3.5.4 Purging the Loading Pump To purge the loading pump, opening the purge valve and then press the PURGE key on the pump (item 1, FIGURE 3-5).

  • Page 43: Chromeleon

    CHROMELEON software. The Switchos II has to be installed as described in Chapter 2. The role of the valves of the Switchos II is dependent on the application for which the unit is configured (e.g. if the FAMOS Microautosampler is included in the system).

  • Page 44: Server Configuration

    Server Configuration Setup 4.2.1 Adding and Configuring the Switchos II To communicate with the Loading Pump of the Switchos II Advanced Microcolumn Switching Unit: a) Start the CHROMELEON server, and then start the CHROMELEON Server Configuration. Select the name for the server (if more than one is configured) of which you want to modify the configuration.

  • Page 45: Switchos Ii Controlled By A Rs-232 Com Port

    To verify or change the configuration, double-click on the ‘LC Packings Switchos II SSV’ device (item 2, FIGURE 4-1). Note: If there are not enough COM ports available, the valves of the Switchos II can be controlled by the event outputs of the Loading Pump (Section 4.2.3).

  • Page 46: Switchos Ii Controlled By Event Outputs

    The switching valves are then controlled by the event outputs of the Switchos II loading pump (TABLE 4-1) and EVENT8 can be used to trigger another device (e.g. a mass spectrometer).

  • Page 47: Adding And Configuring The Virtual Channel Driver

    FIGURE 4-6. FIGURE 4-6 Virtual Channel Setup for Column Pressure Readout To monitor other parameters (e.g. the UltiMate Micropump pressure), select a new channel (FIGURE 4-5) and configure the signal as presented in TABLE 4-2. User’s Manual Switchos II D850R3...
  • Page 48 °C 1.00 TrayTemperature Sampler.Temperature Note: a) ULT = UltiMate , FMS = FAMOS , SW II – Switchos II A typical setup is presented FIGURE 4-1. FIGURE 4-7 Example for a typical Virtual Channel Setup Note: Make sure to use the same signal names and the same formulas as indicated in TABLE 4-2.

  • Page 49: Starting Chromeleon

    Software Control Using CHROMELEON The following section provides information how to use the Switchos II Advanced Microcolumn Switching Unit in conjunction with the CHROMELEON software, the LC Packings UltiMate system and the FAMOS Microautosampler. In addition to the steps used to program the Switchos, basic information is provided how to control the other modules.

  • Page 50: Starting The Flow Delivery And The Baseline Monitoring

    Section 4.3.6 provides a programming example. 4.3.6 Creating a Program – An Example The following section provides information how to program the flow rate of the loading pump and how to use the switching valves. User’s Manual Switchos II D850R3...
  • Page 51 Setup the column oven temperature (if applicable) in the Oven Options box. d) Setup the trap column pressure limits of the Switchos II Loading Pump (e.g. 0 bar and 200 bar) and fill in the time table in the Loading_Pump Options box.
  • Page 52 Specify the detector settings (e.g. the data collection rate) in the UV Options box. i) Define the type of injection you want to use and the corresponding injection parameters in the Sampler Options box. 4-10 User’s Manual Switchos II D850R3...
  • Page 53 Acquisition Options box (FIGURE 4-13). FIGURE 4-13 The Acquisition Option Box The number of UV_VIS_n Options boxes to be filled in is dependent on the number of channels that you are monitoring (e.g. the different wavelengths (FIGURE 4-14). User’s Manual Switchos II 4-11 D850R3...

  • Page 54: Changing A Program File

    A CHROMELEON program can easily be changed by a double click on the program name in the CHROMELEON browser. The program appears in a text format. Program lines are inserted by describing the parameter or by using the F8 command. 4-12 User’s Manual Switchos II D850R3...

  • Page 55: Testing The Switchos

    Testing the Switchos CHAPTER 5 5.1 Overview This chapter describes a series of activities that can be used to check the operation of the system and to verify that your Switchos Advanced Microcolumn Switching Unit is operating in an acceptable manner. This chapter includes: •...

  • Page 56: Checking System Components

    Testing the Switchos 5.2 Checking System Components 5.2.1 Solvent Bottle Caps and Degassing Unit The He lines, the regulating valve and connections can be tested by the following procedure: a) Close the Shut-Off Valves (FIGURE 5-1) and apply 3 bar (45 PSI) of He pressure.

  • Page 57: Solvent Selection Valve And Solvent Lines - Fluid Path Test

    Testing the Switchos 5.2.3 Solvent Selection Valve and Solvent Lines – Fluid Path Test To determine if the fluidic path for solvent line is operating properly and if the resistance of the flow path is within the specifications: a) Fill each solvent bottle (with methanol) until the fluid level corresponds to the top of the Switchos housing.

  • Page 58: Switching Valves - Basic Test

    Testing the Switchos 5.2.4 Switching Valves – Basic Test To check the basic function of the Switching Valves and the Valve Controller: a) Set the Switchos in local mode by the Remote/Local switch on the back panel. The LEDs of the two push buttons on the front panel should be illuminated.

  • Page 59: Maintenance And Troubleshooting

    Troubleshooting - discusses a series of activities that should be used to determine the cause of a problem. (Section 6.4). • Spare Parts/Replacement Parts Lists – Presents a listing of components that are used to maintain the unit or to change the configuration (Section 6.5). User’s Manual Switchos II D850R3...

  • Page 60: Maintenance

    In some cases (e.g. replacement of critical components), we recommend that a factory trained service engineer should be called to perform the operation. This will ensure optimal long term performance and maximum uptime. LC Packings provides a broad range of service support activities to ensure that the Switchos Advanced Microcolumn Switching Unit is functioning in a suitable manner.

  • Page 61: Replacing Major Components

    (P/N 160044). The He tubing should be placed in the hole closer to the He outlet. d) Gently pull at each line to make sure that it is secure. Solvent Line He Line He Outlet (Top View) Solvent Inlet FIGURE 6-1. Sparging/Filter Frit User’s Manual Switchos II D850R3...

  • Page 62: Replacing The High Pressure In-Line Filter

    Caution: Make certain that the filter remains in the filter holder when screwing CAUTION the holder in and do not overtighten the filter holder. Overtightening could result in distortion of the filter which may lead to leakage and/or premature failure of the component. User’s Manual Switchos II D850R3...

  • Page 63: Removing The Pump Head

    (e.g. screws ‘2’ and ‘3’) half a turn at a time until the pump head is correctly seated onto the housing. c) Tighten the two other screws. d) Check that the screws that were tightened in step c) are well tightened. User’s Manual Switchos II D850R3...

  • Page 64: Disassembling The Pump Head/Replacing Piston Seals

    Note: Since the springs (item 5; FIGURE 6-5) exert a significant amount of force, care should be taken when the screws are loosened. User’s Manual Switchos II D850R3...

  • Page 65: Replacing The Check Valve Cartridge

    (lower right) and on the outlet of the main piston (upper right) while two PEEK adapters are used on the auxiliary piston (lower and upper left side). User’s Manual Switchos II D850R3...

  • Page 66: Removing The Side Panels

    Solvent Selection Valve. To remove the panels: a) Remove three screws on the back of each side panel (items 1-6, FIGURE 6- FIGURE 6-6. Rear View of Switchos b) Remove the side panels. User’s Manual Switchos II D850R3...

  • Page 67: Removing The Loading Pump

    Solvents such as methanol, acetonitrile, methanol/water (80/20) or acetonitrile/water (80/20) are normally used to clean the valve. See Appendix A for instructions about disassembling/assembling, cleaning and maintenance of the switching valve. User’s Manual Switchos II D850R3...

  • Page 68: Replacing The Main Fuse

    Pull out the fuse holder (item 1, FIGURE 6-7). Fuse FIGURE 6-7. The Fuse Compartment b) Replace the blown fuse by a fuse of identical rating (2 A, Slo Blo fuse). c) Re-install the fuse holder. 6-10 User’s Manual Switchos II D850R3...

  • Page 69: Troubleshooting

    Air in solvent inlet fluid path degassing Defective valves Clean/replace check valves Purge pump, check Air in solvent inlet fluid path Flow rate too high or too degassing low (deviation >10%) Pump out of calibration Contact LC Packings User’s Manual Switchos II 6-11 D850R3...
  • Page 70 If you are making a change in the protocol, change a single parameter at a time and ensure that the effect of each change is well understood. 6-12 User’s Manual Switchos II D850R3...
  • Page 71 No flow sensor for automatic CRP calibration used. c) Used in the micro flow path. d) If a trap column is used in conjunction with the Switchos, this is the time after switching the trap column into the micro flow. User’s Manual Switchos II 6-13 D850R3...

  • Page 72: Spare Parts List

    50 µm I.D. Connecting tubing, Switchos to UltiMate injection 161261 valve, 70 cm length 50 µm I.D. Connecting tubing, Switchos to UltiMate column 161262 compartment, length 50 cm, includes union 160182 30 µm I.D. PEEK shielded fused silica connection tubing µ- 6-14 User’s Manual Switchos II D850R3...

  • Page 73: 10-Port Valve

    (www.lcpackings.com). 6.5.6 Pump Head and Tubing - Inert Pump Head (Titanium Inlays) Part Number Description 161399 Inert Pump Head (Titanium Inlays) 160049 Check Valve Cartridge (1 piece) 160050 Check Valve Cartridges (set of 5) User’s Manual Switchos II 6-15 D850R3...

  • Page 74: Control Cables

    160147 Contact Closure (4x) Cable for FAMOS P5 Cable for controlling Switchos by FAMOS P5 160171 Auxiliaries Cable for controlling Switchos by UltiMate 160172 Event Outputs 160184 Set of Connectors (WAGO Type) 6-16 User’s Manual Switchos II D850R3...

  • Page 75: Specifications

    Flow Rate Range 10 - 500 µL/min Flow Rate < 0.5% Accuracy Pressure Range 0 - 400 bar Pressure indication of µ-column pressure Monitoring Rapid Purge Valve yes ® Instrument CHROMELEON software or standalone Control User’s Manual Switchos II D850R3...

  • Page 76: General

    (e.g., from FAMOS or UltiMate Micropump) Loading Pump by RS-232 serial communication network Options Inert Version all fluidic pathways made out of inert materials, (factory i.e. PAEK, PEEK, PTFE, ceramic, titanium installed) Flow Passage 0.25 mm Diameter User’s Manual Switchos II D850R3...

  • Page 77: Appendix A Switching Valves

    Switching Valves APPENDIX A Overview The LC Packings Switchos II Advanced Microcolumn Switching Unit is equipped ® with two Cheminert Model C2 10-Port Column Switching Valves manufactured by VICI™ Valco Instruments Co. Inc. These valves are designed to offer optimal...

  • Page 78: Maintenance

    Note: The most common problem in the use of the valve with HPLC is the formation of buffer crystals, which are usually water-soluble. After cleaning, it is not necessary to dry the rotor. User’s Manual Switchos II D850R3...

  • Page 79: Reassembly Of The Valve

    Replacement rotor for VALCO C2, 10-Port 162016 Stator VALCO C2, 10-Port Inert Version (PAEK) 161005 Rotor for Switchos™, 10-port injection valve (VALCO C2), INERT 161006 PAEK Stator for SWITCHOS™ 10-port valve (VALCO C2), INERT Note: PAEK = Polyaryletherketone User’s Manual Switchos II D850R3...
  • Page 80 The Switching Valves [This page intentionally left blank] User’s Manual Switchos II D850R3...

  • Page 81: Appendix B Network Identification

    * “ 0” in case the S/N has less than 6 digits Note: If the Device Identifier or Serial Number for the Switchos is incorrectly set, the unit may not be able to communicate with other devices on the network (depending on the control software package). User’s Manual Switchos II D850R3...
  • Page 82 Network Identification [This page intentionally left blank] User’s Manual Switchos II D850R3...

  • Page 83: Appendix C Cleanup Of Proteins

    APPENDIX C Overview This appendix describes a common application of the Switchos II™ Advanced Micro Switching Unit and is provided to give the user an understanding of the power and flexibility of the system. In this application the two 10-port switching valves are used for on-line sample clean-up, digestion of proteins and peptide separation.
  • Page 84 Valve B presented in position 10-1 Microflow from UltiMate Waste FIGURE C-1 Fluidic Setup of the two 10-Port Valves TABLE 1 Description of the Columns Column No. Column Type Ion-exchange Protein Digestion Trap Column Separation Column User’s Manual Switchos II D850R3...

  • Page 85: Appendix D Connecting Fused Silica Capillary

    Overview This appendix provides instructions how to connect a NANO column or other fused silica capillary to the inert (PAEK) switching valves of the Switchos II Advanced Microcolumn Switching Unit. These directions should be used whenever a NANO column or a fused silica capillary is to be connected to the inert (PAEK) switching valves of the Switchos II.

  • Page 86: Fitting Assembly

    (e.g. it should be grabbed by the sleeve, FIGURE D-3). If the sleeve moves laterally, reinstall the fitting into the fitting port and tighten it another 1/8 turn past finger tight. FIGURE D-3 PEEK Sleeve Connector attached to a Nano Column User’s Manual Switchos II D850R3...

  • Page 87: Using Long Peek Hex Style Nuts

    Carefully tighten the hex style nut using a ¼” wrench. Caution: Never use the inert (PAEK) injection/switching valves to pre-assemble a CAUTION fitting and never use any tool to tighten the fingertight nut. This may lead to damage of the inert (PAEK) valve. User’s Manual Switchos II D850R3...

  • Page 88: Spare Parts Lists

    Inert valve, 1 piece 161000 1/16" Universal Fingertight Fitting with double ferrule for Inert valve, 10 each 163915 PEEK ferrules with groove, for inert valves (20 pcs) Notes: a) PAEK = Polyaryletherketone b) PEEK = Poly-etheretherketone User’s Manual Switchos II D850R3...

  • Page 89: Index

    Switchos controlled by .. 4-4 Using the .. 2-6 Nano Column Connecting D-1 Network Identification B-1 FAMOS Installing the Switchos II with… 2-3 Fittings ON-Standby Switch 3-2 Assembly D-2 PEEK Fittings D-2, D-3 Flow Gradient 4-10 Piston Seals (Replacement) 6-6...
  • Page 90 Inert Version 6-13, A-3, D-4 Specifications 7-1 SSV Valve 1-2, 3-3 Standby 3-2 Starting Flow Delivery 3-6, 4-8 Stopping Flow Delivery 3-6 Switching Valves 1-2, A-1 Assembly A-2 Disassembly A-2 Maintenance A-2 Spare Parts A-3 User’s Manual Switchos II D850R3...

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