Fluidic Connections - Switchos Ii To The Hplc System; Preliminary Operations; He Connection - LC Packings SWITCHOS II User Manual

2.6

2.6.1 Preliminary Operations

To connect the Switchos Advanced Microcolumn Switching Unit to an HPLC
system and to prepare the instrument for operation:
• Place the Switchos Advanced Microcolumn Switching Unit in its operating
location, preferably on the left side of the HPLC system. Make sure the
ventilation holes are not obstructed. Allow the instrument to acclimatize for 1
hour.
•
Connect the Helium line (Section 2.6.2).
• Power-up the system. The display of the loading pump and the various LED's
will indicate that the self-test and initialization have been executed. A
detailed discussion about the control and the various display messages of the
loading pump is presented in the 'UltiMate Micropump User's Manual'.
•
Sparge the mobile phase and flush the solvent lines (Section 2.6.3).
•
Prepare the fluidics connection according to your needs. A typical example is
shown in Section 2.6.4 (additional examples are presented in Appendix C).
Caution: Do not use a stainless steel nut and/or ferrule with the inert (PAEK)
CAUTION
injection/switching valves. The use of stainless steel nuts or ferrules may
damage the valve. Use only the supplied fittings (PEEK) and follow the
instructions below. If you are using an inert version, please refer to Appendix D,
for important information about how to connect fused silica tubing to the inert
switching valves.
Caution: Use the fittings supplied with the instrument (Valco) or equivalent
CAUTION
fittings to ensure that the dead volume of the system is minimized. Use of
Rheodyne fittings should be avoided. Rheodyne fittings are designed differently;
they will lead to unswept volume and possible system damage.

2.6.2 He Connection

Connect the Helium line (1/4 " O.D.) that is supplied to the Helium inlet on the
rear panel of the Switchos (FIGURE 2-1). To connect the Helium line of the
Switchos to the Helium line of the UltiMate Capillary HPLC System, use the T-
piece (P/N 161470) that is supplied with the Switchos instrument. The Helium
pressure should be set to approximately 1 bar.
Caution: Do not operate the He lines at a higher pressure than 4 bar (60 psi).
CAUTION
Close the He-shut off valves when the system is in idle state or not used.
User's Manual Switchos
Fluidic Connections - Switchos to the HPLC System
D850R3
Installation and Getting Started
2-9