Perform Rnase P Verification - Thermo Scientific QuantStudio 1 Installation, Use And Maintenance Manual

Perform RNase P
verification
QuantStudio 1 Real-Time PCR System Installation, Use, and Maintenance Guide
™
Thaw, vortex, and centrifuge an RNase P plate
IMPORTANT! Expose the RNase P plate to room temperature for no more than
45 minutes, inclusive of thawing and preparation time.
After thawing, the RNase P plate cannot be refrozen.
1. Remove the RNase P plate from the freezer, then thaw the plate in its packaging.
• Thaw the plate for approximately 5 minutes.
• Use the plate within 30 minutes of thawing.
2. Confirm that the bench, vortex, and centrifuge are clean. Before use, wipe the
vortex and centrifuge using a lint-free tissue.
3. While wearing powder-free gloves, remove the plate from its packaging.
4. Vortex the plate for 5 seconds, then centrifuge at 750–1,000 × g for 2 minutes.
5. Confirm that the liquid in each well is at the bottom of the well and free of
bubbles. If it is not, centrifuge the plate again.
IMPORTANT! Keep the bottom of the plate clean. Fluids and other contaminants
on the bottom of the plate can contaminate the sample block and cause an
abnormally high background signal.
1. In the home screen, touch
Verification.
2. Load the plate into the instrument.
3. Touch Start.
4. When the run is complete and the screen displays Verification Complete, touch
View Results to confirm the status of the run.
Calibration status
Passed
Failed
Chapter 5 Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
Settings4Maintenance and Service4RNase P
Instrument is ready for use.
See "Troubleshoot verification failure" on page 61.
Action
5
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