Decontaminate The Sample Block; Materials Required; Clean The Sample Block - Thermo Scientific QuantStudio 1 Installation, Use And Maintenance Manual

Decontaminate the sample block

Materials required

Clean the sample
block
QuantStudio 1 Real-Time PCR System Installation, Use, and Maintenance Guide
™
Perform this procedure to eliminate fluorescent contaminants from the instrument
sample block. Contamination is generally evident in failed background calibrations
where one or more wells consistently exhibit abnormally high signals.
CAUTION! PHYSICAL INJURY HAZARD. Do not remove the instrument
cover. There are no components inside the instrument that you can safely
service yourself. If you suspect a problem, contact Support.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the
sample block temperature can reach 100°C. Allow it to cool to room
temperature before handling.
CAUTION! Before using a cleaning or decontamination method other than
those recommended by Thermo Fisher Scientific, confirm with Thermo Fisher
Scientific that the proposed method will not damage the equipment.
• Safety glasses
• Powder-free gloves
• Tissue, lint-free
• Cotton or nylon swabs and lint-free cloths
• Pipette (100-µL) with pipette tips
• Deionized water
• Ethanol, 95% solution
• Bleach, 10% solution
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the
sample block temperature can reach 100°C. Allow it to cool to room
temperature before handling.
IMPORTANT!
·
Wear powder-free gloves when you perform this procedure.
·
Always use deionized water to rinse wells after cleaning with bleach or ethanol
solution.
1. Identify the contaminated wells of the sample block (see "Identify
contamination" on page 56).
2. Prepare the instrument and access the sample block.
a. Power off and unplug the instrument, then allow it to cool for 15 minutes.
b. Pull the instrument drawer forward to expose the sample block.
Chapter 6 Maintain the instrument
Decontaminate the sample block
6
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