Thermo Scientific SORVALL RC-5C PLUS User Manual

Thermo Scientific SORVALL RC-5C PLUS User Manual

Superspeed centrifuge

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RC-5C Plus
User´s Manual
Analyze • Detect • Measure • Control™
PN 74559 - 9

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Table of Contents
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Summary of Contents for Thermo Scientific SORVALL RC-5C PLUS

  • Page 1 RC-5C Plus User´s Manual Analyze • Detect • Measure • Control™ PN 74559 - 9...
  • Page 2 OPERATING INSTRUCTIONS  SORVALL RC-5C PLUS Superspeed Centrifuge Thermo Scientific Asheville, North Carolina U.S.A. PN 74559-9 Issued February 2007...
  • Page 3 NOTE highlights essential information. CAUTION WARNING are accompanied by a hazard symbol and appear in the left sidebar near the information they correspond to. © 1996, 1999, 2000, 2003 by Thermo Scientific...
  • Page 4: Important Safety Information

    RC-5C PLUS Important Safety Information Important Safety Information Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure safe operation of this centrifuge, anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual.
  • Page 5: Table Of Contents

    ® SORVALL Centrifuges Table of Contents Page Important Safety Information ........iii Chapter 1.
  • Page 6 RC-5C PLUS Table of Contents Page Chapter 4. OPERATION Setting the Centrifuge Power ON ....... . . 4-1 Opening the Centrifuge Door .
  • Page 7 ® Table of Contents SORVALL Centrifuges Table of Contents (continued) List of Illustrations Figure Page 1-1. Centrifuge Parts Location and Identification ....1-2 3-1. Control Panel .
  • Page 8: Chapter 1. Introduction & Description

    RC-5C PLUS Introduction and Description Chapter 1: INTRODUCTION & DESCRIPTION This manual provides you with the information you will need to operate and maintain your SORVALL ® RC-5C PLUS Superspeed Centrifuge. If you encounter any problem concerning either operation or maintenance that is not covered in the manual, please contact our Technical Support Group for assistance.
  • Page 9 Introduction and Description ® SORVALL Centrifuges The RC-5C PLUS drive system has a directly coupled, fan-cooled brushless dc motor designed to provide long life without the need to change motor brushes. The high-torque motor provides smooth, quiet operation at all speeds, while delivering precise speed control and exceptional acceleration and braking performance.
  • Page 10: Centrifuge Specifications

    RC-5C PLUS Introduction and Description Centrifuge Specifications Run Speed Speed Selection Range (rpm) ....50 to 21 000 Speed Control Accuracy ....±1% or 100 rpm, whichever is greater Maximum Relative Centrifugal Force .
  • Page 11: Centrifuge Accessories

    Introduction and Description ® SORVALL Centrifuges Centrifuge Specifications (continued) Electrical Requirements, continued Receptacles – for 230 V, 50Hz, single phase ... 3-pin IEC 60309 – for all other single phase ....NEMA 6-30R (for NEMA 6-30P grounded plug, supplied) –...
  • Page 12: Chapter 2. Installation

    RC-5C PLUS Installation Chapter 2: INSTALLATION After you receive your centrifuge, inspect it for damage before using it. The RC-5C PLUS centrifuge must be installed in a location that meets all of the electrical, location, and environment requirements that are specified below and on the next page.
  • Page 13: Environment Requirements

    ® Installation SORVALL Centrifuges A qualified technician may change the power cord to meet local electrical code requirements; the green and yellow wire is the ground and must be connected to the centrifuge frame. A dedicated circuit is required. The ON/OFF main power switch is a 30-Amp circuit breaker;...
  • Page 14: Installation

    Installation RC-5C PLUS Installation Install the RC-5C PLUS centrifuge as follows, observing all electrical, W A R N I N G location, and environment requirements listed on the preceding The RC-5C PLUS Centri- pages: fuge weighs 362 kg (800 lb). Take the necessary precautions when in- 1.
  • Page 16: Chapter 3: Controls, Displays & Indicators

    Controls, Displays, and Indicators RC-5C PLUS Chapter 3: CONTROLS, DISPLAYS & INDICATORS This chapter describes the RC-5C PLUS centrifuge controls, displays, and indicators and includes their locations and functions. Controls, Displays, & Indicators The RC-5C PLUS control switches are used to select desired run parameters and, during a run, digital displays indicate actual run conditions, such as estimated sample temperature, rotor speed, remaining or elapsed run time or accumulated integral value.
  • Page 17: Key Switch And Power Switch

    Controls, Displays, and Indicators ® SORVALL Centrifuges Key Switch and Power Switch NORMAL ZONAL The key switch and power switch are located in the upper right- hand corner of the front cabinet panel. The key switch is set using the key that is supplied with the centrifuge; the position of this switch determines the mode of centrifuge operation.
  • Page 18: Time/ ∫Ω

    Controls, Displays, and Indicators RC-5C PLUS Time/ ∫ω dt Switches, Display, & Indicator The Time/ ∫ω dt display shows one of the following values during a run: (1) accumulated run time if the TIMED/HOLD switch is set to HOLD, (2) remaining run time if the TIMED/HOLD switch is set to TIMED and the OFF/ ∫ω...
  • Page 19: Off/Brake Switch

    Controls, Displays, and Indicators ® SORVALL Centrifuges OFF/BRAKE Switch When the OFF/BRAKE switch is set to BRAKE, the rotor will brake (rather than coast) all the way from set speed to 0 rpm at the end of the run, unless the OFF/ARC switch is set to ARC; if this is the case, the rotor will brake from set speed to 768 rpm, then continue to decelerate to 0 rpm at a controlled rate.
  • Page 20: Off/ ∫Ω 2 Dt Switch

    Controls, Displays, and Indicators RC-5C PLUS OFF/ ∫ω dt Switch • If the OFF/ ∫ω dt switch is set to ∫ω dt and the TIMED/HOLD switch is set at TIMED when the START switch is pressed, the TIME display will show accumulated integral value during the run and the run will end when the preselected integral value has accumulated.
  • Page 21: Backlit Advisory Messages

    Controls, Displays, and Indicators ® SORVALL Centrifuges Backlit Advisory Messages OUT OF OVER OVER BRUSHES DOOR BALANCE TEMP SPEED ENTRY ROTOR ∫ w FAULT HOLD ERROR CODE The RC-5C PLUS has nine operator advisory messages on the control panel. These messages are backlit; each message lights only when the condition that it represents exists.
  • Page 22 Controls, Displays, and Indicators RC-5C PLUS DOOR Indicates one of the following: • Pressed START with the centrifuge chamber door open and the key switch set to NORMAL. The centrifuge will not start. Close the chamber door and start the centrifuge. •...
  • Page 23: Diagnostic Indicators

    Controls, Displays, and Indicators ® SORVALL Centrifuges Diagnostic Indicators These indicators, numbered 1 through 16, help troubleshoot a problem when the centrifuge is not operating properly. When any of the indicators light, it means a problem exists that is affecting the operation of the centrifuge.
  • Page 24 Controls, Displays, and Indicators RC-5C PLUS TACHOMETER ERROR The computer has detected changes in rpm that are beyond the normal range, indicating that the tachometer input frequency is unstable. The condition will clear and the light will go out if the problem is momentary.
  • Page 26: Chapter 4. Operation

    RC-5C PLUS Operation Chapter 4: OPERATION This chapter provides step-by-step instructions on how to set the centrifuge power ON, open the chamber door, and perform a run in the normal mode. It also describes the rate control, zonal, and integrator modes of operation and how to precool the rotor. Read and observe the Important Safety Information supplied on page iii at the front of this manual.
  • Page 27 Operation ® SORVALL Centrifuges 1. Set the main power switch to ON. 2. Set the RUN and MAX temperature switches to the desired run temperature and maximum temperature. The MAX temperature setting should be for a value that is at least 5°C higher than the RUN temperature setting.
  • Page 28: Rate Control (Arc) Operation

    Operation RC-5C PLUS 10. Make sure the OFF/ ∫ω dt switch is set to OFF. 11. Set the TIMED/HOLD switch: • For a timed run, set the switch to TIMED, then set the HRS and MIN switches for the desired length of run time. •...
  • Page 29: Zonal Mode Operation

    Operation ® SORVALL Centrifuges Zonal Mode Operation W A R N I N G When loading the rotor, be The zonal mode of operation must be used when dynamically sure not to exceed the maximum com- loading the gradient and sample into a SORVALL ®...
  • Page 30 Operation RC-5C PLUS 9. Set the TIMED/HOLD switch: • For a timed run, set the switch to TIMED, then set the HRS and MIN switches for the desired length of run time. The timer will start as soon as you press the START switch; therefore, include the time it will take to load the rotor in the run time you select.
  • Page 31: Integrator Mode Operation

    Operation ® SORVALL Centrifuges Integrator Mode Operation The integrator mode can be used in place of the timer to control the length of the centrifuge run. The integral value ( ∫ω dt) represents the actual centrifuge force generated by the rotor accumulated over time.
  • Page 32: Integral Recall

    RC-5C PLUS Operation 2. Set the OFF/ ∫ω dt switch to ∫ω 3. Set the TIMED/HOLD switch to TIMED, then set the COEF and EXP switches for the desired integral value (for example, 15 20 means 1.5 x 10 NOTE The TIMED/HOLD switch should never be set at HOLD during an ∫ω...
  • Page 33: Reducing Speed For Loads In Excess Of Design Mass

    Operation ® SORVALL Centrifuges 3. Set a run speed of 2000 rpm. 4. Set the TIMED/HOLD switch to HOLD. 5. Set the RUN temperature switch for desired run temperature. 6. Set the MAX temperature switch for a value higher than the temperature displayed to avoid an overtemperature condition.
  • Page 34: Rcf Calculation

    Operation RC-5C PLUS RCF Calculation The RCF accessory is used to calculate relative centrifuge force (RCF, also known as g-force) values. The accessory allows you to quickly determine the speed at which you must run a particular rotor to achieve a desired RCF value. To calculate the speed at a particular RCF: 1.
  • Page 35: Test Run For Low Speed And Low Temperature Operation

    Operation ® SORVALL Centrifuges Test Run for Low Speed and Low Temperature Operation If the centrifuge is operated at a speed less than 2000 rpm and a temperature 2°C or less, the refrigeration system may have difficulty maintaining the indicated sample temperature within 1°C of the set temperature.
  • Page 36: Entry Errors

    RC-5C PLUS Operation Entry Errors Any of the following conditions will cause the ENTRY ERROR advisory message to light: • The MAX temperature switch is set at or below the value set on the RUN temperature switch. In this case, the MAX temperature that the system will use is the RUN temperature plus seven degrees.
  • Page 38: Chapter 5. Care And Maintenance

    Care and Maintenance RC-5C PLUS Chapter 5: CARE and MAINTENANCE This chapter describes routine maintenance procedures for your RC-5C PLUS Centrifuge. It also includes cleaning and decontamination recommendations and the Service Decontamination Policy. As the user, it is your responsibility to make certain these procedures and recommendations are followed. In addition to these routine procedures, to keep your centrifuge in good working condition and ensure accurate test results, we recommend that you have the centrifuge serviced and have the speed control, timer, tempera- ture control, and rotor imbalance checked annually by a Thermo Field Service Engineer or other qualified...
  • Page 39: Cleaning And Decontamination

    Care and Maintenance ® SORVALL Centrifuges Cleaning and Decontamination W A R N I N G Rotor Chamber If hazardous materials have been processed in the centrifuge, ob- serve all necessary precautions when The rotor chamber should be defrosted and cleaned periodically to you are cleaning or servicing the cen- maintain efficient cooling.
  • Page 40: Led And Indicator Test

    Care and Maintenance RC-5C PLUS LED & Indicator Test Once a year or whenever displayed numbers seem erroneous, you should test the LEDs and indicators on the control panel to make sure they all work. If even one segment of an LED segment does not work, you could get an incorrect reading in the display.
  • Page 41: Emergency Sample Recovery

    Care and Maintenance ® SORVALL Centrifuges Emergency Sample Recovery W A R N I N G If the main power shuts off because of a power failure or a system malfunction while the rotor is spinning, the RC-5C PLUS chamber When the main power shuts door will not unlatch.
  • Page 42 Care and Maintenance RC-5C PLUS ® The SORVALL Product Guide contains descriptions of com- monly used decontamination methods and a chart showing method compatibility with various materials. The Care and Maintenance Section of the centrifuge or rotor instruction manual contains specific guidance about cleaning and decontamination methods appropriate for the product it describes.
  • Page 43 Care and Maintenance ® SORVALL Centrifuges If a centrifuge or rotor to be serviced does not have a Decontamina- tion Information Certificate attached and, in Thermo's opinion presents a potential radioactive or biological hazard, the Thermo representative will not service the equipment until proper decon- tamination and certification is complete.
  • Page 44: Appendix

    RC-5C PLUS Rotor Information Table Appendix APPENDIX...
  • Page 45: Rotor Information Table

    Rotor Information Table SORVALL ® Centrifuges Rotor Information Table W A R N I N G The SORVALL ® RC-5C PLUS can be used with the SORVALL ® rotors that are listed in the table below and on the next page only . Use of another manufacturer's rotor can cause rotor failure which could result in personal injury and/or centrifuge damage.
  • Page 46 RC-5C PLUS Rotor Information Table CRITICAL MAX COM- RADIUS CODE SPEED SPEED PARTMENT ROTOR (rpm) (rpm) FACTOR MASS (g) (cm) (cm) SH-3000 4 350 3 919 9 500 1 805 18.54 9.11 (buckets) SLA-3000 11 000 20 449 2 813 15.13 3.94 SH-3000...
  • Page 48 SORVALL ® Centrifuges Warranty Thermo Scientific makes no warranty of any kind, expressed or implied, except as stated in this warranty policy. ® The SORVALL RC-5C PLUS Superspeed Centrifuge is warranted to be free from defects in material and workmanship for a period of one year from the date of delivery.
  • Page 49 Glossary Rotor Information Table ® RC-5C PLUS SORVALL Centrifuges Glossary Automatic Rate Contr ol. An accessory Overtemperature The maximum estimated mode that controls the acceleration and sample temperature (MAX) allowed during deceleration of a rotor to avoid gradient or a centrifuge run; if the temperature in the pellet disturbance.
  • Page 50 RC-5C PLUS Index Rotor Information Table SORVALL ® Centrifuges Index ∫ω dt message, 3-7 Entry errors, 4-11 ∫ω dt indicator, 3-3 Environment requirements, 2-2 ∫ω dt display, 3-3 FAULT message, 3-7 Accessories, 1-4 Ambient temperature, 1-3, 2-1 Heat output, 1-3 Advisory messages, backlit, 3-6—3-7 HOLD message, 3-7 Cleaning...
  • Page 51 RC-5C PLUS Rotor Information Table Index SORVALL ® Centrifuges Index (continued) Parts replacement, 5-4 Speed/RCF display, 3-2 Power switch, 3-2 Speed switches, 3-2 Precool, rotor, 4-7 START switch, 3-4 STOP switch, 3-4 Rate control operation, 4-3 Relative centrifugal force, 1-3 Temperature, ambient, 1-3, 2-1 RCF, maximum, centrifuge, 1-3 Temperature display, 3-2...
  • Page 52 DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing (instructions on reverse) PLEASE PRINT DECONTAMINATION CERTIFIED BY _____________________________________________________ TITLE/POSITION ___________________________________________________ PHONE ___________________________ FAX ____________________________ DEPARTMENT _________________________________________________ INSTITUTION _____________________________________________ ADDRESS _________________________________________________________________ CITY _____________________________________________ STATE ____________________________________ ZIP _________________________________ INSTRUMENT ____________________________________________________________________ SERIAL NUMBER _____________________________________ ROTOR _________________________________________________________________________ SERIAL NUMBER _____________________________________ PART ______________________________________________________________________________________...
  • Page 53 INSTRUCTIONS When an instrument that has been used with radioactive, patho- and certification has been completed. If an instrument is received at genic, or otherwise hazardous materials requires servicing by Thermo our Service facilities and, in our opinion, poses a radioactive or personnel either at the customer's laboratory or at Thermo facilities, biological hazard, the sender will be contacted for instructions as to the following procedure must be complied with to insure safety of...
  • Page 54 Europea 2002/96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettronici (WEEE). È marcato col seguente simbolo. Thermo Scientific ha stipulato contratti con una o diverse società di riciclaggio/smaltimento in ognuno degli Stati Membri Europei. Questo prodotto verrà smaltito o riciclato tramite queste medesime. Ulteriori informazioni sulla conformità...
  • Page 55 CHINA INDIA JAPAN Tel.: +86 21 68 65 45 88 Tel.: +91 22 55 42 94 94 Tel.: +81 454 53 92 20 +86 10 58 50 35 88 OTHER ASIA PACIFIC COUNTRIES Tel.: +852 28 85 46 13 AUSTRIA BELGIUM FINLAND Tel.: +43 (1) 80 14 00...

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