4.1.11 Flushing And Installing The Columns - Thermo Scientific Dionex ICS-5000+ Installation Instructions Manual

Dionex ICS-5000
2. Set the pump Purge rate to 0.01 mL/min or select the flow rate specified
3. Verify that the Dionex EGC current, the CR-TC voltage, and the
4. Hydrate the suppressor eluent chamber for 5 minutes and then turn off the
5. Disconnect the CES

4.1.11 Flushing and Installing the Columns

Capillary
IC
NOTE
Flushing the Capillary Columns
1. Locate the Dionex IC Cube Tubing Kit (P/N 072186) in the Dionex ICS-
2. To flush the guard column (if included):
84
+
Installation Instructions
in the Quality Assurance Report (QAR) shipped with the column. Set the
Duration to 300 s and click Prime. Using purge mode prevents low-
pressure alarms.
suppressor current are off.
pump flow.
ELUENT IN
the CES from the flow path during column flushing.
For detailed information about a column, refer to the column
manual, included on the Thermo Scientific Reference Library
DVD (P/N 053891).
+
5000 Ship Kit (P/N 075161). The kit contains precision cut tubing and
the fittings required for installing various column configurations.
a. Connect the 125 mm (4.92 in) precision cut tubing, labeled
(P/N 072189), to the
PORT 3
valve. Connect the other end to the guard column.
b. Select the flow rate specified in the Quality Assurance Report (QAR)
shipped with the column and turn on the pump.
c. Set the Dionex EGC current to the setting specified in the QAR and
turn on the Dionex EGC current. Verify that the CR-TC is off.
d. Flush the guard column to waste for 10 minutes.
e. Connect the separator column to the guard column, using the 65 mm
(2.56 in) precision cut tubing (P/N 072188).
line from the injection valve to remove
port (3) of the injection
ELUENT OUT
VALVE
Doc. 065447-02 12/14