2-2
Learning to Create Methods for the Biomek® FX
2.2
Creating a New Method
If using Accounts &
Permissions . . .
You must have Develop
Methods permission to
create methods. Refer to
the Biomek Software
User's Manual, Chapter 2,
Using Accounts &
Permissions, for more
information.
Method View
The method is built
step by step in this
area.
Basic Step
Palette
Displays the steps
available for
insertion into a
method. Additional
steps are available
through other step
palettes.
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In this chapter, you will build a simple method that
transfers liquid between labware. In building this
method, you will:
•
Populate the deck with labware using the
Instrument Setup step.
•
Transfer liquid with a Multichannel Pod using a
Transfer step.
Note:
Using the Multichannel Pod requires
that a Tip Loader ALP is installed on the deck.
•
Transfer liquid with a Span-8 Pod using a Combine step.
Note:
Using the Span-8 Pod requires that a Span-8 Tip Wash ALP is
installed on the deck. A Span-8 Disposal ALP—called TipTrash in the
Deck Editor—is required if using disposable tips.
•
Move labware with the gripper on the Multichannel Pod using a Move
Labware step.
You launched Biomek Software in Chapter 1. You are now going to create a small
method:
Drag and drop an Instrument Setup into the Method View. A new method is
created and assigned the default name Method1 [New] (Figure 2-1).
Figure 2-1. Biomek Software main editor for dual-pod Biomek FX instrument
Tip Loader
The Tip Loader ALP
loads disposable tips
onto a 96-well head or a
384-well head mounted
on a Multichannel Pod.
Refer to the ALPs User's
Manual, Chapter 23, Tip
Loader ALP, for more
information.
Step Configuration Area
When a step is highlighted in the
Method View, the corresponding
configuration appears in this area.
Current Deck Display
Displays the status of the
deck upon completion of
the previous step.