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- Page 1 User’s Manual Biomek NX and NX Laboratory Automation Workstations Multichannel Pod 987892AF April 2019 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 PN 987892AF (April 2019) © 2019 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
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Page 3: Revision History
Warnings and Cautions Found in this Manual • Introduction, Deck • Introduction, removed Optional Devices • Table 2, Troubleshooting the Biomek NX Instrument • CHAPTER 1, Multichannel Pod Troubleshooting CHAPTER 2, Using the Plate Sensor • Figure 3.13, Framing to the corner of four wells •... - Page 4 Revision History 987892AF...
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Page 5: Safety Notice
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative. -
Page 6: Disposal Of Electronic Equipment
Wavelength: 670 Chemical and Biological Safety Normal operation of the Biomek NX may involve the use of materials that are toxic, flammable, or otherwise biologically harmful. When using such materials, observe the following precautions: • Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease. -
Page 7: Maintenance
• Dispose of all waste solutions according to your facility’s waste disposal procedures. • Operate the Biomek NX in accordance with the instructions outlined in this manual, and take all the necessary precautions when using pathological, toxic, or radioactive materials. -
Page 8: China Rohs Caution Label
Biomek NX with care. The warnings and cautions found within this document are listed below. WARNING If the equipment is used in a manner not specified by Beckman Coulter, Inc., the protection provided by the equipment may be impaired. WARNING Dark non-reflective material affects the sensitivity of the light curtain and adversely impact its effectiveness. - Page 9 WARNING The gripper may bend if not taught (framed) properly with the AccuFrame. WARNING Do not connect or disconnect any cable while power is applied to the Biomek NX. 987892AF...
- Page 10 Warnings and Cautions Found in this Manual WARNING Avoid all labware on the Biomek NX deck when removing or installing grippers. It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware.
- Page 11 Safety Notice Warnings and Cautions Found in this Manual WARNING Turn off power to the Biomek NX instrument before attaching or removing AccuFrame from the instrument deck. WARNING Make sure the light curtain is not violated by the AccuFrame cable. If the light curtain is violated, the framing process halts immediately.
- Page 12 Always have the laser module access cover, located on the bar code reader, in place when operating or troubleshooting the laser module. CAUTION If a label is applied by any means other than the Beckman Coulter Print and Apply device, an increase in bad reads or no reads may occur. CAUTION To prevent damage to the shuttle, first place the AccuFrame into the Framing Tool Adaptor.
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Page 13: Table Of Contents
Chemical and Biological Safety, vi Moving Parts, vii Maintenance, vii China RoHS Caution Label, viii Warnings and Cautions Found in this Manual, viii Biomek NX Multichannel Introduction, xxi Instrument Overview, xxi Control Modes, xxi System Components, xxii Towers, xxii Indicator... - Page 14 Restoring Pod Settings, 2-9 Deleting Pod Settings, 2-10 Framing Instructions, 3-1 Overview, 3-1 Framing Deck Positions on the Biomek NX Using AccuFrame, 3-1 Homing All Axes of the Pods, 3-3 Attaching the Framing Fixture to the Pod, 3-4 Installing AccuFrame, 3-5...
- Page 15 Contents Troubleshooting, 3-24 Manually Controlling the Biomek NX in Biomek Software, 4-1 Overview, 4-1 Accessing Manual Control, 4-1 Using Manual Control, 4-2 Homing All Axes of the Pod, 4-2 Moving the Pod to a Specific Deck Position, 4-3 Stopping the...
- Page 16 Contents Framing the Stacker Carousel, B-29 Index Beckman Coulter, Inc. Customer End User License Agreement Warranty and Returned Goods Requirements, 1 Related Documents...
- Page 17 1.10 Installed replaceable grippers, 1-13 1.11 Removable front gripper assembly, 1-14 Hardware Setup for a Biomek NX Multichannel Instrument, 2-2 Hardware Setup showing the configuration of a Multichannel Pod, 2-4 Warnings must be addressed before homing process begins, 2-5 Hardware Setup displaying Enable Plate...
- Page 18 Illustrations Deck Editor, 3-7 Position Properties, 3-7 Confirm, 3-8 Teaching Instructions, 3-8 Deck Editor, 3-10 Position Properties for a Positive Position ALP, 3-11 3.10 Manual Framing Wizard — Warning, 3-11 3.11 Manual Framing Wizard if tips are not already loaded, 3-12 3.12 Manual Framing Wizard if tips are loaded, 3-12...
- Page 19 Illustrations B.10 Stacker Carousel final placement (top view rear position, right side), B-12 B.11 Bar Code Reader Bracket, B-14 B.12 Bar code reader shuttle mount access cover removed, B-15 B.13 Mounting bracket attachment, B-15 B.14 Mounting bar code reader, B-16 B.15 Bar code reader in narrow side scanning position, B-17...
- Page 20 Tables Tables Multichannel Pod Axes Movement, 1-2 Tip compatibility and max volumes on Multichannel Heads, 1-3 Multichannel Pod Troubleshooting, 1-12 Hardware Setup Options, 2-2 Multichannel Pod Properties, 2-6 Troubleshooting Framing, 3-24 Advanced Manual Control selection areas for the Multichannel Pod (Refer to specific subsections for instructional use.), 4-6 System Specifications, A-1...
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Page 21: Biomek Nx Multichannel Introduction
Biomek NX Multichannel Introduction Instrument Overview NOTE Unless otherwise noted, all information in this hardware manual refers to both the Biomek NX and NX instruments. The Biomek NX Multichannel Laboratory Automation Workstation is a multi-axis liquid-handling instrument used in the life sciences or biomedical laboratory. The open-architecture design, along with the extensible operating software, provides a foundation for integrating current and future specific-use components. -
Page 22: System Components
1) form the two upright sections of the base unit along which the pod travels on the X-axis. The host computer connection of the Biomek NX system, plus utility hook-ups and ALP connections, are located on the towers. A status indicator panel with green... -
Page 23: Right Tower Outside
Biomek NX Multichannel Introduction System Components • Electrical plug-ins and CAN connections for active ALPs on the outside of both towers and inside of the right tower (Figure Figure 2 Right tower outside Figure 3 Left tower outside xxiii 987892AF... -
Page 24: Indicator Lights
Biomek NX Multichannel Introduction System Components Figure 4 Right tower inside Indicator Lights A status indicator panel with green and amber indicator lights is built into the main cover and indicates the current operational status of the Biomek instrument and light curtain (refer to... -
Page 25: Deck
Only). Device Controller A Device Controller is a peripheral box attached to the Biomek NX that provides a means to control a number of high voltage (110VAC-220VAC) devices, low voltage (24VDC) devices, and low voltage digital inputs. High voltage devices receive power through an AC Power Input, while low voltage devices receive power through one of the two Controller Area Network (CAN) interfaces located on the back of the Device Controller. -
Page 26: Protective Barriers
System Components Protective Barriers The Biomek NX Multichannel instrument has safety measures to protect the Biomek NX operator. Safety features of the Biomek NX include a front light curtain and side safety shields (Figure NOTE An optional side safety shield allowing device integrations is available. -
Page 27: Light Curtain Protection System
Control in Biomek Software and insert the material no more than 1” past and approximately 21” above the light curtain panel. Make sure the solid amber indicator light changes to blinking amber. The diffuse-reflective perimeter light curtain along the front edge of the Biomek NX instrument is a standard component. NOTE Side safety shields are installed by a Beckman Coulter Service Engineer when the Biomek NX instrument is installed. -
Page 28: Preventive Maintenance
CHAPTER 4, Manually Controlling the Biomek NX in Biomek Software) and the light curtain test rod supplied with Biomek NX. Insert the test rod no more than 1” past and approximately 21” above the front light curtain panel. Check to make sure the solid amber indicator light changes to blinking amber each time the light curtain is violated. -
Page 29: Changing Fuses
Turn off and unplug power to the instrument before changing fuses. Failure to do so can cause electrical shock or equipment damage. The Biomek NX instrument uses any AC power source between 100V and 240V. The fuse holder holds two European fuses and one North American fuse. -
Page 30: Replacing Fuses In Tower
Biomek NX Multichannel Introduction Troubleshooting Figure 6 Replacing fuses in tower Gently pull old fuses from the carrier by hand. Gently place new fuses into carrier by hand. NOTE Use 250v 3.15 amp 5x20 Slow-Blow type fuses for replacement. Replace the fuse carrier into the AC power input module. -
Page 31: Multichannel Pod
CHAPTER 1 Multichannel Pod Overview The Multichannel Pod is a self-contained working unit on the Biomek NX Multichannel Laboratory Automation Workstation (Figure 1.1). The Multichannel Pod is a full-microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions (refer to Interchangeable Heads). -
Page 32: Pod Movements
Multichannel Pod Overview The main components of the Multichannel Pod are (Figure 1.2): • Head — Holds mandrels and tips for performing full-plate replication (refer to Interchangeable Heads). • Gripper — Grips and repositions labware by gripping along the long side of the labware (refer to Gripper). -
Page 33: Control Modes
Multichannel Pod. Manual control associated with the operation of the Multichannel Pod is accessed through Biomek Software. NOTE Refer to CHAPTER 4, Manually Controlling the Biomek NX in Biomek Software, for information on manually controlling the Multichannel Pod. Interchangeable Heads An interchangeable, multichannel head is attached to the bottom of the pod to perform a specific liquid-handling procedure. -
Page 34: Removing Heads
NOTE A wrist ground strap, which must be attached to the instrument deck (base plate) using the “alligator” clip, is supplied with the Biomek NX instrument (Figure 1.3). Figure 1.3 Wrist ground strap attached to the deck 1. -
Page 35: Bottom Cover Removed From Pod
NOTE Choosing Change Head moves the D-axis to –0.6 cm and extends the grippers. Turn off power to the Biomek NX instrument. Position the strap portion of the wrist ground strap snugly around the wrist. Clamp the “alligator” clip of the wrist ground strap to the instrument deck (base plate) (Figure 1.3). -
Page 36: Plunger Screws And Shoulder Screws Removed From
Multichannel Pod Interchangeable Heads Using the 9/64 Allen wrench provided, remove the four plunger screws from the plunger plate (Figure 1.5) in the sequence designated in Figure 1.6. CAUTION Before removing the fourth shoulder screw, take hold of the head firmly to make sure it does not fall once all screws are removed. -
Page 37: Installing Multichannel Heads
A wrist ground strap, which must be attached to the instrument deck (base plate) using the “alligator” clip, is supplied with the Biomek NX instrument. The same method is used to install the 96-Channel 20 μL Head, 96-Channel 200 μL Head, and 384 Channel 30 μL Head. -
Page 38: Chamfer Locations On A Multichannel Head
Multichannel Pod Interchangeable Heads To install a head: , select the appropriate Multichannel Hardware Setup NOTE A Multichannel Pod is identified with a 96 or 384. Choose Change Head NOTE Choosing Change Head moves the D-axis to –0.6 cm and extends the grippers. Turn off power and make sure the plastic bottom cover is removed (Figure 1.4). -
Page 39: Sequence For Installing Four Plunger Screws And Four Shoulder Screws On All Multichannel Heads
Multichannel Pod Interchangeable Heads Figure 1.8 Sequence for installing four plunger screws and four shoulder screws on all multichannel heads 1. Shoulder 1 5. Plunger 1 2. Shoulder 2 6. Plunger 2 3. Shoulder 3 7. Plunger 3 4. Shoulder 4 8. -
Page 40: Gripper
The Multichannel Pod incorporates a gripper tool (gripper) (Figure 1.9) that grasps and moves labware from one location on the Biomek NX deck to another. The gripper can: • move labware no taller than 2.35 inches in height. • move stacks of standard height labware no more than four plates high (maximum 2.35 inches). -
Page 41: Framing The Gripper
WARNING The gripper may bend if not taught (framed) properly with the AccuFrame. The gripper is framed during installation by a Beckman Coulter Service Engineer. The framing process for the gripper must be repeated when: • A head is changed. -
Page 42: Troubleshooting
Multichannel Pod Troubleshooting Troubleshooting WARNING Do not connect or disconnect any cable while power is applied to the Biomek NX. Perform the troubleshooting techniques provided in Table 1.3 when necessary. NOTE In the case of any other pod-related problems, contact a Beckman Coulter Service Engineer. -
Page 43: Replacing Gripper Fingers
Removing Gripper Fingers WARNING Avoid all labware on the Biomek NX deck when removing or installing grippers. It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware. -
Page 44: Installing Gripper Fingers
Installing Gripper Fingers WARNING Avoid all labware on the Biomek NX deck when removing or installing grippers. It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware. - Page 45 Multichannel Pod Troubleshooting Orient the gripper so that the front of the gripper faces the outside and gripper pads face the inside of the Multichannel Pod (Figure 1.11). Grasping both sides of the gripper, push the gripper straight up into the Multichannel Pod. NOTE A click can be heard and felt when the gripper fingers are seated in the Multichannel Pod.
- Page 46 Multichannel Pod Troubleshooting 1-16 987892AF...
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Page 47: Configuring The Biomek Nx Multichannel In Biomek Software
CHAPTER 2 Configuring the Biomek NX Multichannel in Biomek Software Overview The Biomek NX Multichannel Laboratory Automation Workstation must be configured in Biomek Software to ensure proper operation. tells Biomek Software what devices, pods, and Hardware Setup heads to expect on the instrument by providing a connection between the instrument and the software. -
Page 48: Understanding The Options In Hardware Setup
Provides the Biomek NX Multichannel instrument with a point of reference from which to make subsequent moves. Home position is left, back. The pod must be homed each time the Biomek NX instrument is powered on. Add Device Installs a device. -
Page 49: Configuring The Biomek Nx Multichannel Instrument
Configuring the Biomek NX Multichannel in Biomek Software Configuring the Biomek NX Multichannel Instrument Configuring the Biomek NX Multichannel Instrument The Biomek NX Multichannel instrument is configured in to relate information Hardware Setup about the configuration of the multichannel pod on the system to the software and to specify the communications port to which it is connected. -
Page 50: Configuring A New Head
Configuring the Biomek NX Multichannel in Biomek Software Configuring a Multichannel Pod Configuring a New Head When a head on a Multichannel Pod is changed, the must be changed appropriately. Hardware Setup CAUTION If the hardware configuration is not updated using Hardware Setup, hardware crashes or inaccurate liquid transfer may occur. -
Page 51: Setting Multichannel Pod Properties
Warning NOTE Additional Warnings may appear depending on deck configuration and the type of head installed on the Biomek NX Multichannel instrument. Address all warnings appropriately and choose OK to continue. , choose to establish a new D-axis limit. -
Page 52: Multichannel Pod Properties
NOTE Gripper X offset is read-only. Last Validation Set by a Beckman Coulter Service Engineer, using the Set Validation button. Maximum X, Y, Z, D The furthest position the pod may move to along the X-, Y-, Z-, and D-axes (relative to the Home position). -
Page 53: Using The Plate Sensor
Unload Speed Controls the pod speed when unloading tips, based on a percentage of the maximum pod speed. Recommended that this be modified only by a Beckman Coulter Service Engineer. Using the Plate Sensor A plate sensor is available on the gripper. -
Page 54: Saving, Restoring, And Deleting Settings
Configuring the Biomek NX Multichannel in Biomek Software Saving, Restoring, and Deleting Settings Figure 2.4 Hardware Setup displaying Enable Plate Sensor 1. Enable Plate Sensor Figure 2.5 Error for no plate sensed Saving, Restoring, and Deleting Settings The axes limits and pod settings may be saved, restored, and deleted using the... -
Page 55: Restoring Pod Settings
Configuring the Biomek NX Multichannel in Biomek Software Saving, Restoring, and Deleting Settings Figure 2.6 Save Configuration Create a name for the current configuration. If desired, enter a description of the configuration. Choose . The settings are saved. Restoring Pod Settings... -
Page 56: Deleting Pod Settings
Configuring the Biomek NX Multichannel in Biomek Software Saving, Restoring, and Deleting Settings Choose appears (Figure 2.7). Restore Settings Load Settings Figure 2.7 Load Settings 1. Desired settings to load are selected here. Select the desired settings. Choose . The following... -
Page 57: Delete Settings
Configuring the Biomek NX Multichannel in Biomek Software Saving, Restoring, and Deleting Settings Figure 2.9 Delete Settings 1. Select desired settings to delete here. Select the desired settings to delete. Choose . The following appears (Figure 2.10). Confirm Figure 2.10 Confirmation to delete stored configuration Choose . - Page 58 Configuring the Biomek NX Multichannel in Biomek Software Saving, Restoring, and Deleting Settings 2-12 987892AF...
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Page 59: Framing Instructions
Framing the Gripper Framing Deck Positions on the Biomek NX Using AccuFrame The AccuFrame is a tool used for framing ALP and labware positions on the Biomek NX deck (Figure 3.1). Framing using the AccuFrame tool requires no human judgment of alignment and is reproducible. -
Page 60: Accuframe Framing Tool
AccuFrame and read by Biomek Software as needed. To frame deck positions of the Biomek NX using AccuFrame, a framing fixture is attached to the head of the Multichannel Pod. When framing multiple position ALPs such as the 1 X 3 Passive ALP, all positions on the ALP should be framed to increase precision. -
Page 61: Homing All Axes Of The Pods
• Make sure the gripper on the Multichannel Pod is retracted. Prior to framing the Biomek NX deck, all axes must be homed. Homing the pod gives Biomek NX a point of reference from which to make subsequent moves. Home position is left and back. While it is necessary to home the pod after the Biomek NX instrument is powered on, it is not necessary to home the pod each time the host computer is turned on or the software is accessed. -
Page 62: Attaching The Framing Fixture To The Pod
Framing Instructions Framing Deck Positions on the Biomek NX Using AccuFrame Figure 3.2 Warning to address before homing process begins Choose on each after confirming that the actions have been Warning Information addressed appropriately. Attaching the Framing Fixture to the Pod After homing the pod, the framing fixture must be installed on the pod used for framing. -
Page 63: Installing Accuframe
AccuFrame is positioned on the ALP, the framing process is completed through the software. WARNING Turn off power to the Biomek NX instrument before attaching or removing AccuFrame from the instrument deck. Turn off power to the Biomek NX instrument before connecting the AccuFrame. -
Page 64: Framing The Position
Framing Instructions Framing Deck Positions on the Biomek NX Using AccuFrame WARNING Make sure the light curtain is not violated by the AccuFrame cable. If the light curtain is violated, the framing process halts immediately. WARNING Make sure the AccuFrame cable does not interfere with pod movement. -
Page 65: Deck Editor
Framing Instructions Framing Deck Positions on the Biomek NX Using AccuFrame Figure 3.4 Deck Editor In Biomek Software, open the deck that requires framing in the . Verify that it Deck Editor reflects the current configuration of ALPs on the physical deck. If it does not reflect the current... -
Page 66: Teaching Instructions
Framing Instructions Framing Deck Positions on the Biomek NX Using AccuFrame Figure 3.6 Choose Auto Teach. A Confirm similar to appears. NOTE This moves the pod to the position being taught. The framing probe should be above the AccuFrame in that position. -
Page 67: Selecting Appropriate Teaching Instructions
Framing Instructions Framing Deck Positions on the Biomek NX Using AccuFrame Choose from , or for appropriate teaching instructions (refer Shift deck Shift ALP Shift position Selecting Appropriate Teaching Instructions). If the coordinates displayed appear reasonable, choose appears again, Position Properties and the position is framed for the selected pod. -
Page 68: Manually Framing Deck Positions On The Biomek Nx
Framing Instructions Manually Framing Deck Positions on the Biomek NX Manually Framing Deck Positions on the Biomek NX is a wizard-type interface that is used to manually frame deck Biomek NX Manual Framing Wizard positions, primarily for using high density labware. Since the wells of high density labware are... -
Page 69: Position Properties For A Positive Position Alp
In Name, verify that the ALP is assigned a unique name. Figure 3.10 Choose Manual Teach. Biomek NX Manual Framing Wizard opens with a Warning ( NOTE On the left side of Manual Framing Wizard, a list of steps required to complete the teaching process is displayed. -
Page 70: Manual Framing Wizard If Tips Are Not Already Loaded
Framing Instructions Manually Framing Deck Positions on the Biomek NX Choose appears. Figure 3.11 Figure 3.12 appears, Next Manual Framing Wizard — Setup depending on whether or not tips are loaded onto the pod. Figure 3.11 Manual Framing Wizard if tips are not already loaded Figure 3.12 Manual Framing Wizard if tips are loaded... -
Page 71: Framing To The Corner Of Four Wells
Framing Instructions Manually Framing Deck Positions on the Biomek NX , select the appropriate labware type of the labware placed in the position Line tips up against to frame. Choose to continue to the next screen. Next Biomek NX Manual Framing Wizard — Teach X,Y appears. -
Page 72: Using The Graphic Alignment Tool
Framing Instructions Manually Framing Deck Positions on the Biomek NX Figure 3.14 Framing to the center of a well To align the tips in the X- and Y-axes with the wells of the microplate on top of the ALP, lower the tips in the Z-axis until they are approximately 1 mm above the top of the ALP. - Page 73 Framing Instructions Manually Framing Deck Positions on the Biomek NX Figure 3.15 Manual Teaching the X- and Y- axes 1. Graphic alignment tool— The graphic alignment tool is a visual representation of the tip (small circle) and the microplate wells or corner.
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Page 74: Using The Delta Value And Directional Buttons
Framing Instructions Manually Framing Deck Positions on the Biomek NX Visually verify the position of the tips on the pod in relation to the physical position of the wells of the microplate on top of the ALP. If the tips are still not accurately positioned above the... -
Page 75: Framing The Z-Axis
Framing Instructions Manually Framing Deck Positions on the Biomek NX To use the delta value and directional buttons to position the tip: , select the magnitude of change applied to the tips each time a directional button is Delta selected (Figure 3.16). - Page 76 Framing Instructions Manually Framing Deck Positions on the Biomek NX Figure 3.17 Manual Teaching (teach Z) , select the magnitude of change applied to the pod each time a directional button is Delta selected (Figure 3.17). NOTE The default Delta value is 0.05 cm. If the tip is a considerable distance above the position, increase the distance traveled by increasing the Delta value (maximum setting is 1.0 cm).
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Page 77: Framing The Gripper
Choosing Cancel loses all changes to the deck, including framing information, since the Deck Editor was opened. Framing the Gripper A Beckman Coulter Service Engineer frames the gripper during system installation; however, it may be necessary to repeat the framing procedure if: • the head is changed. -
Page 78: Warning To Make Sure Gripper Is Ready For Framing
Framing Instructions Framing the Gripper Figure 3.18 Hardware Setup Choose Frame Gripper When a appears, make sure the topics that appear in the are addressed and Warning Warning choose (Figure 3.19). Figure 3.19 Warning to make sure gripper is ready for framing (Figure 3.20), select a previously framed deck position and frame the gripper to Pick Position... -
Page 79: Pick Previously Framed Deck Position To Frame The Grippers
Framing Instructions Framing the Gripper Figure 3.20 Pick previously framed deck position to frame the grippers Choose . This moves the pod to that position and extends the grippers. Advanced Manual (Figure 3.21) and (Figure 3.22) appear side by side. Control Frame Gripper NOTE... -
Page 80: Moving Pod Into Gripping Position
3.23), and squeeze the fingers until they firmly touch the AccuFrame inside the notches at the bottom of the AccuFrame (Figure 3.24). If necessary, refer to CHAPTER 4, Manually Controlling the Biomek NX in Biomek Software, for instructions on moving the gripper. NOTE Both front and rear gripper fingers must touch at the same time when squeezing, which usually necessitates a move along the Y-axis. -
Page 81: Correct And Incorrect Gripper Framing Alignment
Framing Instructions Framing the Gripper Figure 3.24 Correct and incorrect gripper framing alignment 1. Correct Gripper Alignment — Bottom of gripper finger aligned to bottom of AccuFrame and aligned to the inside of cut-out. 2. Incorrect Gripper Alignment — Top of gripper finger aligned to top of cut-out of AccuFrame without aligning to inside of cut-out. -
Page 82: Testing Gripper Framing Accuracy
Perform the troubleshooting techniques provided in Table 3.1 when necessary. NOTE In the case of any other framing-related problems, contact a Beckman Coulter Service Engineer. Table 3.1 Troubleshooting Framing Then AccuFrame power light not on Check the CAN connection to make sure the AccuFrame is connected to Biomek NX instrument. -
Page 83: Manually Controlling The Biomek Nx In Biomek Software
(Figure 4.1) Manual Control Instrument > Manual Control Information appears briefly as the connection is made with the Biomek NX instrument, immediately followed (Figure 4.2). Manual Control NOTE Manual Control is available only when a method is not being executed. If a need for manual control is realized during a method run, stop the method using the Stop button or Snap Continuation button (refer to the Biomek Software Version 4.1 User’s Manual (PN B30026) or the Biomek Software Version... -
Page 84: Using Manual Control
When attempting to use the pod, error messages result until the pod is homed. NOTE While it is necessary to home the pod after the Biomek NX instrument is powered on, it is not necessary to home the pod each time the host computer is turned on or the software is accessed. -
Page 85: Stopping The Pod
Manually Controlling the Biomek NX in Biomek Software Using Manual Control To home the pod: Choose appears (Figure 4.2). Instrument > Manual Control Manual Control Choose . The following appears (Figure 4.3): Home All Axes Warning Figure 4.3 Warnings must be addressed before homing process begins. -
Page 86: Viewing The Firmware
Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Viewing the Firmware Version shows the current firmware version for installed devices, pods, and main firmware. Get Version To view the firmware version: Choose appears (Figure 4.2). Instrument > Manual Control... -
Page 87: Extending And Retracting The Unloading Tips
Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control • Performing Absolute Moves for the Pod • Extending and Retracting the Gripper • Unloading Tips (Windows 10 only) Figure 4.5 Overview Advanced Manual Control for a Multichannel Pod 1. -
Page 88: Advanced Manual Control Selection Areas For The Multichannel Pod (Refer To Specific Subsections For Instructional Use.)
Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Table 4.1 Advanced Manual Control selection areas for the Multichannel Pod (Refer to specific subsections for instructional use.) Area Description Absolute Move Builds a vector to an absolute coordinate from the current coordinate. - Page 89 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Choose Advanced Controls Select for the pod appears (Figure 4.5). The current position is Pod1 Advanced Manual Control shown in Current Position Choose to close Close Advanced Manual Control...
- Page 90 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Performing Relative Moves for the Pod Relative moves allow the pod to move from its current location to anywhere on the deck. Relative moves are created in the using the values, or the vector can be manually edited.
- Page 91 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Choose to close Exit Manual Control Setting Delta Values for the Pod value is the amount of change in an axis that is applied to the when a...
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Page 92: Vector Builder
Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Figure 4.6 Vector Builder • Right = Positive value X = • Left = Negative value X = right motion of the pod left motion of the pod •... -
Page 93: Enter Absolute Move Coordinates
Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Figure 4.7 Enter Absolute Move Coordinates Enter the , and values for the desired position. NOTE Minimum and Maximum X, Y, Z, and D values displayed in Hardware Setup indicate how far the pod is able to move. - Page 94 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control Extending and Retracting the Gripper to extend or retract the gripper when changing the head on the pod. Advanced Manual Control CAUTION Select Move Z-Max to move the pod to its highest point before extending the gripper.
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Page 95: Specifications
APPENDIX A Specifications System Specifications Table A.1 System Specifications Item Description Environment Indoor use only Power Requirements US: 100 - 120VAC, 60Hz Europe: 200 - 240VAC, 50Hz Pneumatic Requirements Air Pressure: 60 - 100 psi of compressed air — 414 - 690 kPa of compressed air Air Flow: 2.25 cfm @ 60 psi —... - Page 96 Specifications System Specifications Table A.1 System Specifications (Continued) Item Description Biomek Controller Biomek Software Version 4.1 The following specifications are the minimum requirements needed. Processor: Intel Core i5-2400 (3.1 GHZ) or better Memory: 2 GB Hard Drive: 250 GB or better Optical Drive: DVD Additional Software: Microsoft SQL Server 2005 Edition Operating System: Windows 7, 64-bit with scripting enabled...
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Page 97: Using A Stacker Carousel
Configuring the Stacker Carousel in Hardware Setup • Framing the Stacker Carousel Integrating the Stacker Carousel NOTE Install only in conjunction with the Biomek NX model incorporating Plexiglas safety shields along the sides of the instrument in lieu of side light curtains. 987892AF... -
Page 98: Positioning The Stacker Carousel On The Mounting The Stacker
Using a Stacker Carousel (Windows 7 Only) Integrating the Stacker Carousel Figure B.1 Stacker Carousel Integrating the Stacker Carousel into the system requires: Positioning the Stacker Carousel on the Deck • • Mounting the Stacker Carousel • Attaching the Side Shield •... -
Page 99: Carousel
While it is possible to pipette liquid to and from a a 96-well plate on the Stacker Carousel shuttle, it is not possible to pipette liquid to and from a 386-well plate that is sitting on the Stacker Carousel shuttle. To position the Stacker Carousel: Position the Stacker Carousel outside the Biomek NX deck (Figure B.3). -
Page 100: Stacker Carousel Integration
Using a Stacker Carousel (Windows 7 Only) Integrating the Stacker Carousel Figure B.3 Stacker Carousel Integration Mounting the Stacker Carousel Mounting the Stacker Carousel to the Biomek NX requires attaching an alignment bracket (Figure B.4) and a riser plate (Figure B.5). -
Page 101: Attaching Alignment Bracket
Attach the upper alignment bracket and the riser plate to prepare for positioning and leveling the Stacker Carousel as follows: Choose the right or left side of the Biomek NX instrument on which the Stacker Carousel will be located. Choose the deck position the shuttle will occupy (Figure B.2). - Page 102 Verify that the riser plate is set firmly on the Biomek NX alignment bracket and the table. NOTE If the Biomek NX has leveling feet attached, place the 4 standoffs underneath each of the feet on the riser plate (Figure B.5).
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Page 103: Attaching Riser Plate
Using a Stacker Carousel (Windows 7 Only) Integrating the Stacker Carousel Figure B.5 Attaching Riser Plate 1. Riser alignment pins 2. Riser Plate 3. Standoffs 987892AF... -
Page 104: Stacker Attachment
Figure B.6 Stacker Attachment Level the stacker. Adjust the height of the stacker until the distance between the Biomek NX deck surface and the top of the flat surface of the stacker shuttle measures 5 in. ± 1/8 in. NOTE If necessary, refer to the Stacker Carousel User’s Manual (PN 148598), for instructions on how to... -
Page 105: Attaching The Side Shield Components To Biomek Nx
(Figure B.7), attach the lower side shield components to the top side shield. Attach the shield to the Biomek NX using the screws provided. NOTE Use the same attachment procedure for either side of the instrument. Figure B.7 Attaching the side shield components to Biomek NX 1. -
Page 106: Aligning The Stack Shuttle
Using a Stacker Carousel (Windows 7 Only) Integrating the Stacker Carousel Align the shuttle to load and dispense labware through the Plexiglas side panel opening (Figure B.8) and (Figure B.9). Figure B.8 Aligning the stack shuttle B-10 987892AF... -
Page 107: Front View Of Stacker Integration
Using a Stacker Carousel (Windows 7 Only) Integrating the Stacker Carousel Figure B.9 Front view of stacker integration B-11 987892AF... -
Page 108: Troubleshooting
Using a Stacker Carousel (Windows 7 Only) Integrating the Stacker Carousel Connect the Stacker Carousel (Figure B.10). NOTE To connect the Stacker Carousel, connect the supplied serial cable from the side panel of the Stacker Carousel to a PC Controller’s communication port. If more than two communication ports are required, install the extra serial ports in the PC at this time. - Page 109 Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Integrating the Bar Code Reader A bar code reader may be mounted to the Stacker Carousel to scan any one of the four sides of a microplate. NOTE For maximum scanning reliability, it is recommended that labels be applied to the narrow sides of the microplate.
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Page 110: Reader
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.11 Bar Code Reader Bracket 1. Bar code reader 4. Bar code reader mount 2. Alignment bracket adjustment screws 5. M4 x 6 flat 3. Bar code reader cable lamp Attaching Bar Code Reader to Shuttle Attach the bar code reader to the Stacker Carousel shuttle as follows: Remove the mount access cover... -
Page 111: Bar Code Reader Shuttle Mount Access Cover
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.12 Bar code reader shuttle mount access cover removed 1. Shuttle mount access cover 2. Bar Code Reader shuttle mount access Screw mount standoffs onto shuttle arm assembly mount points inside shuttle mount access (Figure B.13). -
Page 112: Mounting Bar Code Reader
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Attach bar code reader bracket assembly to bar code reader mount base (Figure B.14). Figure B.14 Mounting bar code reader 1. Bar code reader bracket assembly 2. Bar code reader mount base 3. -
Page 113: Bar Code Reader In Narrow Side Scanning
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.15 Bar code reader in narrow side scanning position 1. Laser beam Figure B.16 Bar code reader in narrow side scanning position 1. Laser beam B-17 987892AF... -
Page 114: Bar Code Reader In Wide Right Side Scanning
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.17 Bar code reader in wide right side scanning position 1. Laser beam Figure B.18 Bar code reader in wide right side scanning position (bottom view) 1. Laser beam B-18 987892AF... -
Page 115: Bar Code Reader In Rear Narrow Side Scanning
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.19 Bar code reader in rear narrow side scanning position 1. Laser beam Figure B.20 Bar code reader in rear narrow side scanning position (bottom view) 1. Laser beam B-19 987892AF... -
Page 116: Bar Code Reader In Wide Left Side Scanning
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.21 Bar code reader in wide left side scanning position 1. Laser beam Figure B.22 Bar code reader in wide left side scanning position (bottom view) 1. Laser beam B-20 987892AF... -
Page 117: Bar Code Reader In Front Narrow Side Scanning
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.23 Bar code reader in front narrow side scanning position 1. Laser beam Figure B.24 Bar code reader in front narrow side scanning position (bottom view) 1. Laser beam B-21 987892AF... -
Page 118: Bracket
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Figure B.25 Bar code reader attachment bracket 1. Bracket fasteners 4. Bar code reader laser beam 2. Bracket 5. Bar code reader 3. Fastener collars Applying Power to the Bar Code Reader CAUTION To avoid serious damage to the instrument, make sure that the laboratory site voltage/frequency matches the voltage/frequency that was ordered for the... -
Page 119: Beam
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader Turn on the Stacker Carousel power switch. Aligning the Laser Beam WARNING Avoid direct exposure to the laser beam. Never look directly into the laser beam, and never leave the laser on, open, or unattended. WARNING Always have the laser module access cover, located on the bar code reader, in place when operating or troubleshooting the laser module. -
Page 120: Bar Code Reader - Laser Beam Position Over Bar Code
Using a Stacker Carousel (Windows 7 Only) Integrating the Bar Code Reader To verity correct laser alignment, use the pendant to move the shuttle with the labeled microplate through the laser beam. The laser beam should pass over all vertical bars on the bar code label simultaneously. -
Page 121: Adjust Laser Alignment As Necessary
If label needs to be applied to the microplate, it is recommended that a Beckman Coulter Print and Apply device be used to position the label on the microplate properly (Figure B.28). -
Page 122: Setup
Configuring the Stacker Carousel in Hardware Setup to install, configure, and remove Stacker Carousels. For more information on Hardware Setup , refer to CHAPTER 2, Configuring the Biomek NX Multichannel in Biomek Software. Hardware Setup is accessed from within Biomek Software. -
Page 123: New Devices
Using a Stacker Carousel (Windows 7 Only) Configuring the Stacker Carousel in Hardware Setup Figure B.29 New Devices Check and choose . The device is displayed under Stacker Carousel Install Stacker Carousels the left pane. NOTE The first Stacker Carousel added to the instrument is named Stacker1, the second is named Stacker2, and so forth. -
Page 124: Hardware Setup Showing The Configuration View For A Stacker Carousel
Using a Stacker Carousel (Windows 7 Only) Configuring the Stacker Carousel in Hardware Setup Configuring Stacker Carousels To configure Stacker Carousels: , select the desired stacker under Stacker Carousels in the left pane of Hardware Setup and the configuration view appears in the right pane (Figure B.31). -
Page 125: Stacker
Using a Stacker Carousel (Windows 7 Only) Framing the Stacker Carousel Removing Stacker Carousels to remove Stacker Carousels: , right-click the desired device under Hardware Setup Stacker Carousels Choose . The device is removed from the installed stackers under Remove Device Stacker in the left pane. -
Page 126: Adding The Accuframe And Framing Tool Adaptor To The Stacker
Turn off power to the Biomek NX instrument before attaching or removing AccuFrame from the instrument deck. Turn off power to main unit before connecting the AccuFrame. Plug the AccuFrame into any available CAN port on the Biomek NX tower. B-30 987892AF... - Page 127 Using a Stacker Carousel (Windows 7 Only) Framing the Stacker Carousel WARNING Make sure the light curtain is not violated by the AccuFrame cable. If the light curtain is violated, the framing process halts immediately. WARNING Make sure the AccuFrame cable does not interfere with pod movement. Turn on power to main unit.
- Page 128 Using a Stacker Carousel (Windows 7 Only) Framing the Stacker Carousel B-32 987892AF...
- Page 129 Absolute Move setting values for the Multichannel Pod, using in Advanced Manual Control, 4-10 delta values AccuFrame using in Manual Teach, 3-16 calibrated by Beckman Coulter Service Device Controller Engineer, overview, 1-xxv defined, installing the, troubleshooting, 3-24 Advanced Manual Control...
- Page 130 4-10 accessing, performing relative moves, choosing Frame Gripper, 3-20 configuring a Multichannel Pod in, configuring a new head in, configuring the Biomek NX MC, defined, framing (teaching) Multichannel Pod properties in, Multichannel, saving, restoring, and deleting settings manually controlling, moving to a specific deck position,...
- Page 131 In no event shall Beckman Coulter, Inc.’s liability to the user exceed the amount paid by the user to Beckman Coulter, Inc. hereunder. The user assumes full responsibility for the results obtained from the use of this system and related documentation and for application of such results.
- Page 132 Company. Parts replaced during the warranty period are warranted to the end of the instrument warranty. Note: Performance characteristics and specifications are only warranted when Beckman Coulter replacement parts are used.
- Page 134 PN B300026 Preinstallation Manual Instruments Integration PN B20063 Manual Biomek Software User’s PN A93392 Manual Version 4.2 Biomek NX and NX PN C40390 Instructions for Using the Laboratory Automation Span-8 Tube Expanding and Workstations Span-8 Pod Biomek NX Laboratory Tube Holding Devices...
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