Beckman Coulter BiomekFX Quick Start Manual
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Beckman Coulter BiomekFX Quick Start Manual

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Beckman Coulter BiomekFX Quick Start Manual

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  • Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
  • Page 2 Workstations Quick-Start Guide Beckman Coulter PN 987838 Revision AE December 2009 Beckman Coulter, Inc 250 S. Kraemer Boulevard, Brea, CA 92821 Copyright © 2009 Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 3 Except as expressly provided herein, Beckman Coulter, Inc. makes no other warranty, whether oral or written, expressed or implied, as to any matter whatsoever, including but not limited to those concerning merchantability and fitness for a particular purpose, nor is freedom from any patent owned by Beckman Coulter, Inc. or by others to be inferred.
  • Page 4 Warranty and Returned Goods Requirements All standard Beckman Coulter, Inc. policies governing returned goods apply to this product. Subject to the exceptions and upon the conditions stated below, the Company warrants that the products sold under this sales agreement shall be free from defects in...
  • Page 5 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 6 Table of Contents Warranty and Returned Goods Requirements ........iii Table of Contents .
  • Page 7 Quick-Start Guide Summary ........3-17 Beckman Coulter, Inc.

  • Page 8: Introduction

    ® Introducing the Biomek Laboratory Automation Workstation Introduction Note: Unless otherwise noted, all information in this quick-start guide refers to both the Biomek® FX and FX instruments. ® Welcome to the Biomek FX Laboratory Automation Workstation. This quick-start guide is designed to help you learn to use Biomek Software with your Biomek FX.

  • Page 9: How To Use This Quick-Start Guide

    Biomek® FX and FX-P Laboratory Automation Workstations User’s or the Manual Biomek Software User’s Manual for more information on these important concepts. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 10: Biomek® Fx

    Introducing the Biomek® FX Laboratory Automation Workstation ® Biomek The Biomek FX (Figure 1-1) is a multiaxis liquid-handling instrument used in the laboratory. Its open-architecture design, along with the extensible operating software, provides a foundation for integrating current and future specific-use components. The Biomek FX may have one or two pods and a variety of operating components, such as heads, ALPs, and third party devices.

  • Page 11: Pods And Heads

    Note: Refer to the ALPs User’s Manual for detailed information on the ALPs on your system. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 12: Light Curtain And Other Protective Barriers

    Introducing the Biomek® FX Laboratory Automation Workstation 1.2.3 Light Curtain and Other Protective Barriers WARNING: To reduce the risk of personal injury, safety shields and light curtain must be in place before operating. The safety shields and light curtain prevent entry into the work area during machine movement. WARNING: Dark non-reflective material affects the sensitivity of the light curtain and adversely impact its effectiveness.

  • Page 13: Biomek Software

    Project and Instrument menus (refer to Chapter 30.5, Using the Chapter 3, Using the Editors While Creating Step Palette Builder for Methods). more information. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 14: Turning On The Biomek Fx

    Accounts & Permissions To launch the Biomek Software: is included in the Biomek 1. Choose Start>All Programs>Beckman Coulter>Biomek Software. Software installation and provides control over 2. If Accounts & Permissions is enabled (see sidebar), a dialogue box appears...

  • Page 15: Homing All Axes Of The Biomek Fx Pod

    Proceed through this quick-start guide while using the links to the user’s manuals to learn more about the Biomek FX liquid-handling system. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 16: Overview

    Learning to Create Methods for ® the Biomek Overview In this chapter you will be given information and instructions for building a small method using Biomek Software. A method is a series of steps that control the operation of your Biomek FX. The method you complete in this chapter represents basic operations and is intended simply to familiarize you with the Biomek FX, and to create a basic understanding of method-building opportunities.

  • Page 17: Creating A New Method

    Additional the previous step. steps are available through other step palettes. Figure 2-1. Biomek Software main editor for dual-pod Biomek FX instrument Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 18 Learning to Create Methods for the Biomek® FX As you can see, two steps automatically appear in every method created and executed by the Biomek Software: • Start — the first step in a method; allows Variables creation of global variables that are applicable A variable is a value that to the entire method.

  • Page 19: Configuring The Instrument Setup Step

    Figure 2-2. Instrument Setup step in the Method View Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 20 Learning to Create Methods for the Biomek® FX To configure the Instrument Setup step for your new method: CAUTION: Do not place labware other than a tip box on a tip loader position (TL#). 1. In Deck, make sure the default deck layout (Deck1) is selected. The Instrument Setup step uses a deck layout as a map for the placement of labware, ALPs, and external hardware devices, such as stacker carousels.
  • Page 21 13. Choose OK to close Labware Properties. Figure 2-5. Labware Properties for Dest1 14. Double-click the labware on P12 and name it Dest_S8 in Labware Properties. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 22 Learning to Create Methods for the Biomek® FX 15. In Labware contains, select Unknown. 16. Choose OK to close Labware Properties. Note: The default settings in Labware Properties will be used for the two tip boxes; however, you can refer to the Biomek Software User’s Manual, Chapter 16.2.5.1, Configuring Labware Properties for Tips, to learn more about configuring a tip box.

  • Page 23: Configuring A Transfer Step For Multichannel Pod

    • Section 2.4.3, Configuring Source Labware • Section 2.4.4, Configuring Destination Labware Current Deck Display Figure 2-7. Transfer step inserted into the Method View Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 24: Configuring Tip Handling

    Learning to Create Methods for the Biomek® FX 2.4.2 Configuring Tip Handling Tip Handling To configure tip handling: Configuring Tip Handling is not always 1. Make sure a Multichannel Pod is selected in Use pod. If your instrument has two required because the pods, the left pod is Pod1 and the right pod is Pod2.

  • Page 25: Configuring Source Labware

    The source labware configuration is complete and the main editor now looks like Figure 2-9. Figure 2-9. Transfer step with source labware configured Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 26: Configuring Destination Labware

    Learning to Create Methods for the Biomek® FX 2-11 2.4.4 Configuring Destination Labware To configure the labware named Dest1 as destination labware in the Transfer step: 1. Before you configure the destination labware, click the down arrow in Transfer Transfer Details Details located just above the Current Deck Display to collapse that section.
  • Page 27 Figure 2-10. Transfer step with Destination configured Note: The method view changes to reflect the amount transferred when the next step is configured. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 28: Configuring A Combine Step For Span-8 Pod

    Learning to Create Methods for the Biomek® FX 2-13 Configuring a Combine Step for Span-8 Pod If your instrument does not have a Span-8 Pod, skip this section and continue to the Combine Step next. A Combine step loads The Combine step transfers liquid from one or more sources to a single destination. tips, aspirates and You are going to insert and configure a Combine step using a Span-8 Pod into the dispenses liquid, and...

  • Page 29: Configuring Tip Handling

    Tip Handling to collapse the configuration. This allows more room for the step configuration (Figure 2-12). Use this arrow to collapse and expand Tip Handling. Figure 2-12. Combine step with Tip Handling collapsed Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 30: Configuring Source Labware

    Learning to Create Methods for the Biomek® FX 2-15 2.5.3 Configuring Source Labware To configure the labware named Dest1 as source labware in the Combine step: 1. Click on Click here to add a source. 2. Click on the Dest1 labware sitting on P5 in the Current Deck Display. Note: The information you supplied during Instrument Setup is displayed in the source labware configuration.
  • Page 31 Learning to Create Methods for the Biomek® FX The source labware configuration is complete and the main editor now looks like Figure 2-13. Figure 2-13. Combine step with source labware configured Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 32: Configuring Destination Labware

    Learning to Create Methods for the Biomek® FX 2-17 2.5.4 Configuring Destination Labware To configure the labware named Dest_S8 as destination labware in the Combine step: 1. Click on Dest_S8 sitting on P12 in the Current Deck Display to select the 384- well microplate as the destination.
  • Page 33 Multichannel Pod. If your instrument does not have a Multichannel Pod, skip to Section 2.7, Step, to learn how to learn how long the method will Using the Finish take to run. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 34: Configuring A Move Labware Step

    Learning to Create Methods for the Biomek® FX 2-19 Configuring a Move Labware Step Note: If your instrument does not have a Multichannel Pod, you cannot move labware because your instrument does not have a gripper; continue to Section 2.7, Using the Finish Step.

  • Page 35: Using The Finish Step

    Displayed as Failed when there is an error. Figure 2-17. Quick-Start method with error Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 36: Saving A Method

    Learning to Create Methods for the Biomek® FX 2-21 Saving a Method After you’ve clicked on Finish step, you can save your method. Methods and 1. Choose File>Save from the toolbar. Save Method appears (Figure 2-18). Projects Saving a method automatically checks in the method, creating a record of the revision that...
  • Page 37 Learning to Create Methods for the Biomek® FX Figure 2-19. Quick-Start method saved and named Move on to Section 2.9, Method, to run your saved Quick-Start method. Running a Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 38: Running A Method

    Learning to Create Methods for the Biomek® FX 2-23 Running a Method Next you will run the method you just created using the simulator. The simulator Purging Air from allows you to watch the method on your screen without actually running the method Span-8 Pod on an instrument.
  • Page 39 (Figure 2-22) appears on top of the main editor. Simulation display button Click to toggle the view of the simulator on and off. Figure 2-21. Biomek FX simulator displayed Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 40 Learning to Create Methods for the Biomek® FX 2-25 Figure 2-22. Deck confirmation prompt for Quick Start method 5. Choose OK. The prompt closes and the method continues. View the method as it proceeds in the simulator. The method can also be followed in the Method View as steps are highlighted in green as they are executed.
  • Page 41 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 42: Overview

    Using the Editors While Creating Methods Overview In this chapter you will be given information and instructions for using the editors in the Biomek Software. The editors allow you to customize a method to your special requirements. You can go beyond the predefined project items and instrument configurations;...

  • Page 43: General Description Of Editors

    Figure 3-1. If you would like to learn more about a specific editor, refer to the specified chapter for more information. You will work with three of the more commonly used editors and Manual Control in this quick-start guide. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 44: Project Menu

    Using the Editors While Creating Methods 3.2.1 Project Menu The editors on the Project menu (Figure 3-1) allow project items to be created, copied, revised, and renamed. They include the following: • Labware Type Editor — allows you to add new labware types, copy labware types, delete labware types, rename labware types, and edit labware type properties (refer to the Biomek Software User’s Manual, Chapter 8, Creating and Modifying Tip and Labware Types).

  • Page 45: Instrument Menu

    (decks, framing tools, pod settings) as import files (.imp) (refer to the Biomek Software User’s Manual, Chapter 4.4, Sharing Instrument Settings Using the Import/Export Utility). Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 46: Creating New Labware In The Labware Type Editor

    Using the Editors While Creating Methods Creating New Labware in the Labware Type Editor The Labware Type Editor is used to create labware definitions so you can use Labware labware that is not predefined in Biomek Software in your methods. Definitions Assume that you have new labware that is very similar to a BCFlat96, but some When creating a new...
  • Page 47 Labware Type Editor (Figure 3- Copy of BCFlat 96 renamed to NewLabwareQuickStart Figure 3-4. NewLabwareQuickStart in Labware Type Editor Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 48 Using the Editors While Creating Methods 4. Double-click the NewLabwareQuickStart graphic. NewLabwareQuickStart appears (Figure 3-5). Figure 3-5. NewLabwareQuickStart Note: Each piece of labware in Labware Types has its own mini-editor similar to Figure 3-5 to allow specifications to be revised and saved. 5.

  • Page 49: Creating A New Pattern

    To create a new well pattern based on the NewLabwareQuickStart labware you have created: 1. From the Project menu, choose Well Pattern Editor. Well Patterns appears (Figure 3-6). Figure 3-6. Well Patterns Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 50 Using the Editors While Creating Methods 2. Choose New. New Pattern appears (Figure 3-7). Figure 3-7. New Pattern 3. Highlight NewLabwareQuickStart and choose OK. Figure 3-8 appears. Figure 3-8. Well Patterns based on Pattern 96 Biomek® FX and FX-P Laboratory Automation Workstations Quick-Start Guide Artisan Technology Group - Quality Instrumentation ...
  • Page 51 Figure 3-9. Wells chosen for the new pattern 5. Choose Rename to rename the new pattern. Rename Pattern appears (Figure 3-10). Figure 3-10. Rename Pattern Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 52 Using the Editors While Creating Methods 3-11 6. Enter LabwareQuickStart and choose OK. Figure 3-11 appears. Figure 3-11. New pattern renamed 7. Choose Save to save LabwareQuickStart in Well Patterns. Note: Refer to the Biomek Software User’s Manual, Chapter 12, Creating Well Patterns, for more information.

  • Page 53: Creating A New Deck

    To create a new deck in the Deck Editor: appropriate positions to perform liquid-handling 1. Choose Instrument>Deck Editor. Deck Editor appears (Figure 3-12). operations and manipulate labware. A Beckman Coulter Service Engineer frames the Biomek FX instrument during system installation. It may be necessary to repeat the...
  • Page 54 Using the Editors While Creating Methods 3-13 5. Drag and drop Span8WashLeft to any location capable of supporting it (Figure 3-14). A second Span8WashLeft has been added to the deck. This deck is now the default deck and is available in the Instrument Setup step. Figure 3-14.

  • Page 55: Manually Controlling The Pod

    1. Choose Instrument>Manual Control. Manual Control appears (Figure 3- 16). Here is where you can choose to which position you want to move a pod. Figure 3-16. Manual Control Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 56 Using the Editors While Creating Methods 3-15 2. Select Advanced Controls and highlight Pod1 or Pod2. Advanced Manual Control: for the selected Pod appears (Figure 3-18). Figure 3-17. Pod1 highlighted in Advanced Controls Figure 3-18. Advanced Manual Control for a Multichannel Note: Advanced Manual Control looks different if the selected pod is a Span-8 Pod.
  • Page 57 7. From the toolbar, choose Close to close the quick-start method. From the File menu, choose Close. The method closes and you are now ready to create a method of your own. Beckman Coulter, Inc. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 58: Quick-Start Guide Summary

    Using the Editors While Creating Methods 3-17 Quick-Start Guide Summary Congratulations! You’ve just completed this quick-start guide. You’ve completed: • a small method • steps using the editors • steps to manually control the Biomek FX If you’ve used the links to other manuals, you’ve also learned where to find hardware information on the Biomek FX, ALPs, and more information on Biomek Software.
  • Page 59 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 60 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

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