Loading The Ladder And The Samples - Agilent Technologies DNA 7500 Manual Kit

5
Agilent DNA 7500 and DNA 12000 Assay Protocol

Loading the Ladder and the Samples

Loading the Ladder and the Samples
1 Pipette 1 μl of the yellow-capped ladder vial
2 In each of the 12 sample wells pipette 1 μl
For optimal results, samples should be of pH 6 to 9 and should not have an ionic content
N O T E
greater than twice that of a typical PCR buffer.
3 Set the timer to 60 seconds.
4 Place the chip horizontally in the adapter of the IKA vortex mixer and make
Wrong vortexing speed
C AU T I O N
If the vortexing speed is too high, liquid spill that disturbs the analysis may occur for
samples generated with detergent containing buffers.
➔
5 Vortex for 60 seconds at 2400 rpm.
6 Refer to the next topic on how to insert the chip in the Agilent 2100
16
(yellow ) in the well marked with the ladder
symbol .
of sample (used wells) or 1 μl of deionized water
(unused wells).
sure not to damage the buldge that fixes the chip during vortexing.
Reduce vortexing speed to 2000 rpm!
Bioanalyzer. Make sure that the run is started within 5 minutes.