Loading The Gel-Dye Mix - Agilent Technologies DNA 7500 Manual Kit

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Agilent DNA 7500 and DNA 12000 Assay Protocol

Loading the Gel-Dye Mix

Loading the Gel-Dye Mix
Before loading the gel-dye mix, make sure that the base plate of the chip priming station is
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in position (C) and the adjustable clip is set to the highest position. Refer to
the Chip Priming Station"
1 Allow the gel-dye mix to equilibrate to room
2 Take a new DNA chip out of its sealed bag and place
3 Pipette 9.0 μl of the gel-dye mix at the bottom of the
When pipetting the gel-dye mix, make sure not to draw up particles that may sit at the
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bottom of the gel-dye mix vial. Insert the tip of the pipette to the bottom of the chip well
when dispensing. This prevents a large air bubble forming under the gel-dye mix. Placing
the pipette at the edge of the well may lead to poor results.
4 Set the timer to 30 seconds, make sure that the plunger is positioned at
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temperature for 30 minutes before use. Protect the
gel-dye mix from light during this time.
the chip on the chip priming station.
well marked and dispense the gel-dye mix.
1 ml and then close the chip priming station. The lock of the latch will
click when the chip priming station is closed correctly.
on page 8 for details.
"Setting up