Essential Measurement Practices - Agilent Technologies DNA 7500 Manual Kit

Essential Measurement Practices

• Handle and store all reagents according to the instructions on the label of
the individual box.
• Avoid sources of dust or other contaminants. Foreign matter in reagents
and samples or in the wells of the chip will interfere with assay results.
• Keep all reagents and reagent mixes refrigerated at 4 °C when not in use.
• Allow all reagents and samples to equilibrate to room temperature for
30 min before use.
• Protect dye and dye mixtures from light. Remove light covers only when
pipetting. The dye decomposes when exposed to light and this reduces the
signal intensity.
• Always insert the pipette tip to the bottom of the well when dispensing the
liquid. Placing the pipette at the edge of the well may lead to poor results.
• Use a new syringe and electrode cleaners with each new kit.
• Use loaded chips within 5 min after preparation. Reagents might evaporate,
leading to poor results.
• Do not touch the Agilent 2100 Bioanalyzer during analysis and never place
it on a vibrating surface.
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