Loading The Marker - Agilent Technologies DNA 7500 Manual Kit

5 Press the plunger of the syringe down until it is
6 Wait for exactly 30 seconds and then release the
7 Visually inspect that the plunger moves back at
8 Wait for 5 seconds, then slowly pull back the
9 Open the chip priming station.
10 Pipette 9.0 μl of the gel-dye mix in each of the
Protect the gel-dye mix from light. Store the gel-dye mix at 4 °C when not in use for more
N O T E
than 1 hour.

Loading the Marker

1 Pipette 5 μl of green-capped marker (green )
Do not leave any wells empty, or the chip will not run properly. Add 5 µl of green-capped
N O T E
DNA marker (green ) plus 1 µl of deionized water to each unused sample well.
held by the clip.
plunger with the clip release mechanism.
least to the 0.3 ml mark.
plunger to the 1 ml position.
wells marked .
into the well marked with the ladder symbol
and into each of the 12 sample wells.
Agilent DNA 7500 and DNA 12000 Assay Protocol
Loading the Marker
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