Negative Ion Mode Checkout Conditions - Agilent Technologies 6490 Installation Manual

Negative Ion Mode Checkout Conditions

These checkout conditions are used in Negative Ion Mode checkout.
Table 15
Time
Initial
0.1
1
2
2.01
3.7
Table 16
Injection volume
Autosampler temperature
Needle wash flushport
Needle wash location
Table 17 Source
ESI (6420/
6430/6460)
Gas Temp 310°C
Gas Flow 13 L/min
Nebulizer 25 psi
Sheath Gas Temp
Sheath Gas Flow
Nozzle Voltage
Agilent 6400 Series Triple Quad LC/MS System Installation Guide
HPLC Pump
%A (water)
90
90
2
2
90
Stop
HPLC Autosampler
AJS (6460)
200°C
6 L/min
35 psi
400°C
12 L/min
1000 V
Negative Ion Mode Checkout Conditions
%B (Methanol)
10
10
98
98
10
2 µL
4°C
75:25 methanol:water
Flushport, 10 seconds
AJS (6470) AJS (6490)
200°C 210°C
6 L/min 17 L/min
35 psi 25 psi
400°C 400°C
12 L/min 12 L/min
1000 V 2000 V
Reference 6
Flow
0.5 mL/min
0.5 mL/min
0.5 mL/min
0.5 mL/min
0.5 mL/min
AJS (6495)
210°C
17 L/min
25 psi
400°C
12 L/min
2000 V
109