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To verify the operation of the G1971B APPI source
There is no sensitivity checkout with the G1971 APPI source on the Triple
Quadrupole LC/MS. To verify its proper operation, run a manual tune with the
G2432A APCI/APPI Calibration Solution.
Note that Auto Tune is not supported with this source.
1 Install the G2432A APCI/APPI Calibration Solution.
2 Check the tuning of the APPI source:
3 Save the tune file.
Agilent 6400 Series Triple Quad LC/MS System Installation Guide
a In Data Acquisition program, set Context to Tune.
b Open the auto tune file that was generated with the electrospray or
multimode source.
c In the Manual Tune tab, mark the Calibrant on check box, and then
acquire the data.
d Verify that the masses in the MS1 and MS2 tabs are correct for the
loaded source before you manually tune the peak.
e Verify that you have sufficient abundance for the tune peaks, that the
tune peaks have peak widths of 0.7 ±0.05 amu, and that mass
assignments are within ±0.15 amu of the target mass assignments.
If needed, click Adjust Gain & Offset to establish the Mass Axis and
Peak Width values. Repeat these for both MS1 and MS2 tabs.
Installation and Verification of Other Sources
To verify the operation of the G1971B APPI source
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