Step 2. Reset The Collision Cell Gas Flow Rate - Agilent Technologies 6490 Installation Manual

3 Installation Completion and System Verification

Step 2. Reset the collision cell gas flow rate

Step 2. Reset the collision cell gas flow rate

Do not follow these steps for the 6470A and 6495A/B. The gas flow for the 6470A and
N O T E
6495A/B LC/MS systems are set at the factory and the value is permanently saved in the
instrument Smart Card. Most parameters such as the collision cell gas flow rate work with
values set at the factory.
If the collision gas flow rate needs to be reset, such as after the installation of a
SmartCard, see
page 91.
Most parameters such as the collision cell gas flow rate work with values set at
the factory. In case the default flow rate does not give a high vacuum reading
in the acceptable range, follow the steps in this topic to:
High Vacuum measurements with Collision Cell gas turned off can vary depending upon
N O T E
how long ago the LC/MS instrument was pumped down.
For best results, allow the instrument to pump overnight at Autotune settings, but with
Collision Cell gas turned OFF, before taking the High Vacuum measurement.
Acceptable ranges are listed in
Table 1
6400 Series Model
6490
6410 with G2573A, 6430/6460 1.8 to 2.2 Torr
6410/6420
54
"To confirm the collision cell gas flow rate (6470A and 6495A/B only)"
• Determine the optimal flow rate (DAC steps) to achieve the correct high
vacuum gauge reading.
• Enter this value into a Data Acquisition configuration file.
Acceptable vacuum ranges
Agilent 6400 Series Triple Quad LC/MS System Installation Guide
Table 1.
Rough Vacuum High Vacuum
(CC Gas OFF)
2.2 to 3.7 Torr 2.0 to 3.0 × 10
2.0 to 5.0 × 10
1.8 to 2.2 Torr 2.0 to 5.0 × 10
High Vacuum
(CC Gas ON)
-6 -5
Torr 5.0 to 5.8 × 10
-6 -5
Torr 2.0 to 3.0 × 10
-6 -5
Torr 2.0 to 4.0 × 10
on
Torr
Torr
Torr