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3
Installation Completion and System Verification
Step 1. Condition the Agilent LC instrument
Step 1. Condition the Agilent LC instrument
Before you begin, check that you have:
1 Flush the LC system.
2 Remove any column(s), install a restriction capillary, and bypass the flow
3 If required by your degasser model, connect A1/A2 degasser channels in
4 Use a hand-held controller or the Data Acquisition program to set up the
5 Set up the pump module to deliver HPLC grade isopropanol for 5 minutes at
6 Set up the pump module to deliver the premixed LC flushing solvent
50
•
18 M, HPLC grade deionized water (p/n 8500-2236)
•
high-purity HPLC grade isopropanol
•
premixed LC flushing solvent (50:25:15:10
isopropanol/acetonitrile/cyclohexane/dichloromethane)
(p/n G1969-85026)
•
high-purity HPLC grade acetonitrile (p/n G2453-85050)
•
high-purity HPLC grade methanol (p/n 8500-1867)
•
high purity formic acid (p/n G2453-85060)
•
for 6470/6495: RRHD Zorbax Eclipse Plus C18 trapping column
(p/n 959757-302)
This flushing procedure is very effective at reducing background
contaminants from the LC systems when they are interfaced with a mass
spectrometer. The flushing procedure must be done for each solvent
channel to ensure contaminants are removed from separate degasser and
pumping channels.
cell for the UV detector, unless it is rated for high-pressure (400 bar)
operation. Route the outlet capillary to a waste container.
series and B1/B2 degasser channels in series. Use the c-tubes included with
the degasser.
Don't try to re-use solvents used for the flushing procedure.
pump module to deliver deionized water for 15 minutes at 3 mL/minute to
remove any salt buffers. Do 5 injections with deionized water to clean the
injector path and sample loop.
3 mL/minute. Do 5 injections with isopropanol to clean the injector path
and sample loop.
(50:25:15:10 isopropanol/acetonitrile/cyclohexane/dichloromethane) for 15
Agilent 6400 Series Triple Quad LC/MS System Installation Guide
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