4
Installation and Verification of Other Sources
To verify the operation of the G1978B multimode source
To verify the operation of the G1978B multimode source
Note that Auto Tune is not supported with this source.
1 Install the G1969-85020 MMI-L Low Concentration Tuning Mix.
2 Check the tuning of the multimode source:
3 Save the tune file.
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a In Data Acquisition program, set Context to Tune.
b Open the auto tune file that was generated with the electrospray or
multimode source.
c In the Manual Tune tab, mark the Calibrant on check box, and then
acquire the data.
d Verify that the masses in the MS1 and MS2 tabs are correct for the
loaded source before you manually tune the peaks.
e Verify that you have sufficient abundance for the tune peaks, that the
tune peaks have peak widths of 0.7 ±0.05 amu, and that mass
assignments are within ±0.15 amu of the target mass assignments.
If needed, click Adjust Gain & Offset to establish the Mass Axis and
Peak Width values. Repeat these for both MS1 and MS2 tabs.
Agilent 6400 Series Triple Quad LC/MS System Installation Guide