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3
Tuning
Calibrating the LC/TQ (Autotune)
Calibrating the LC/TQ (Autotune)
6475A and Ultivo
An autotune can be run with the following ion sources: ESI, AJS ESI, MMI, and
APCI.
1 In the Method Editor window, click the TQ tab.
2 Click Tune > Autotune in the left pane.
3 Click
This button locks control of the LC/TQ instrument. When tune has control of
the LC/TQ instrument, a single sample run or a worklist cannot be started.
When clicked, tune setpoints are applied. If an action is not selected within the
timeout period, the system will go back to the method setpoints.
4 Select a polarity.
•
•
5 Click
a To stop the tune during operation, click
6 When the tune completes, review the report(s).
7 Click
Agilent Triple Quadrupole LC/MS System User Guide
Request tune control.
If only Positive or Negative polarity is selected, subsequence acquisition
methods are only available with that polarity.
If Both is selected, the system will automatically run in the positive mode
followed by the negative mode.
Autotune the instrument.
Release tune control to release control of the LC/TQ instrument.
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