Solvent Information - Agilent Technologies G1314B User Manual

Agilent 1260 infinity variable wavelength detector

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Solvent Information

Observe the following recommendations on the use of solvents.

Flow Cell

Avoid the use of alkaline solutions (pH > 9.5 ) which can attack quartz

and thus impair the optical properties of the flow cell.

Prevent any crystallization of buffer solutions. This will lead into a

blockage/damage of the flow cell.

If the flow cell is transported while temperatures are below 5 °C, it must

be assured that the cell is filled with alcohol.

Aqueous solvents in the flow cell can built up algae. Therefore do not

leave aqueous solvents sitting in the flow cell. Add small % of organic

solvents (for example, acetonitrile or methanol ~5 %).

Solvents

Brown glass ware can avoid growth of algae.

Always filter solvents, small particles can permanently block the

capillaries. Avoid the use of the following steel- corrosive solvents:

• Solutions of alkali halides and their respective acids (for example,

• High concentrations of inorganic acids like nitric acid, sulfuric acid

• Halogenated solvents or mixtures which form radicals and/or acids, for

Agilent 1260 Infinity VWD User Manual

lithium iodide, potassium chloride, and so on).

especially at higher temperatures (replace, if your chromatography

method allows, by phosphoric acid or phosphate buffer which are less

corrosive against stainless steel).

example:

→ 2COCl

2CHCl

+ O

This reaction, in which stainless steel probably acts as a catalyst,

occurs quickly with dried chloroform if the drying process removes the

stabilizing alcohol.

+ 2HCl

Appendix