Setting Up An Analysis; Before Using The System - Agilent Technologies G1314B User Manual

Setting up an Analysis

This chapter can be used for
• preparing the system,
• to learn the set up of an HPLC analysis and
• to use it as an instrument check to demonstrate that all modules of the
• Learn about special settings
All descriptions are based on the Agilent ChemStation B.02.01. Newer versions may look
N O T E
different.

Before Using the System

Solvent Information
Observe recommendations on the use of solvents in chapter "Solvents" in
the pump's reference manual.
Priming and Purging the System
When the solvents have been exchanged or the pumping system has been
turned off for a certain time (for example, overnight) oxygen will
re- diffuse into the solvent channel between the solvent reservoir, vacuum
degasser (when available in the system) and the pump. Solvents containing
volatile ingredients will slightly lose these. Therefore priming of the
pumping system is required before starting an application.
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system are correctly installed and connected. It is not a test of the
instrument performance.
Using the Detector

Setting up an Analysis

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