Zeiss Axioskop 2 plus Operating Manual page 120

Carl Zeiss
Fig. 3-33 Height measurement
3-52
OPERATION
Quantitative microscopy
Example:
Upper (3-33/1) and lower side (3-33/2) of a thin
section were focused using a dry objective
(n = 1.0).
M
The indicated intervals of the mechanical fine drive
are 15.0 and 24.5, which means that d' = 9.5 µm
with an interval of 1 µm.
The refractive index of the specimen area n
been assumed as n
section height is:
d = d' x
Axioskop 2 plus
Axioskop 2 mot plus
= 1.5. Therefore, the thin
P
n 1.5
P
= 9.5 µm x = 14.25 µm
n
1
M
B 40-075 e
has
P
02/01