Objectives; Fig. 1-3 Objective - Zeiss Axioskop 2 plus Operating Manual

Routine microscope
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Axioskop 2 plus
Axioskop 2 mot plus
When immersion objectives are used, the air between the cover slip and the objective is replaced with a
liquid, which is immersion oil in most cases. The plastic oiler containing 20 ml of 581 N immersion oil (n
= 1.515) is particularly suitable for this purpose.
To prevent oil contamination of the specimen when the nosepiece is turned, the resilient mounts of the
immersion objectives can be locked in their lifted position by turning them to the right (do not forget to
unlock them again!).
The following objectives are ideal for the Axioskop 2 plus and Axioskop 2 mot plus microscopes:
Microscopy technique
Transmitted-light brightfield
Phase contrast
Transmitted-light brightfield
Phase contrast
Transmitted-light brightfield
Phase contrast
Transmitted-light brightfield
B 40-075 e
10/03
INSTRUMENT DESCRIPTION
Objective
Magnification /
Num. Aperture
A-Plan
A-Plan
5x/0.12
A-Plan
10x/0.25
A-Plan
20x/0.45
A-Plan
40x/0.65
A-Plan
100x/1.25 Oil
A-Plan
10x/0.25 Ph 1
A-Plan
20x/0.45 Ph 2
A-Plan
40x/0.65 Ph 2
A-Plan
100x/1.25 Oil Ph 3
Achroplan
Achroplan
4x/0.10
Achroplan
10x/0.25
Achroplan
20x/0.45
Achroplan
40x/0.65
Achroplan
50x/0.90 Oil
Achroplan
63x/0.80
63x/0.95
Achroplan
Achroplan
100x/1.25 Oil
Achroplan
100x/1.25 Oil Iris
Achroplan
10x/0.25 Ph 1
Achroplan
20x/0.45 Ph 2
Achroplan
40x/0.65 Ph 2
Achroplan
100x/1.25 Oil Ph 3
Plan-Neofluar
Plan-Neofluar
2.5x/0.075
Plan-Neofluar
5x/0.15
Plan-Neofluar
10x/0.30
Plan-Neofluar
20x/0.50
Plan-Neofluar
40x/0.75
Plan-Neofluar
100x/1.30 Oil
Plan-Neofluar
10x/0.30 Ph 1
Plan-Neofluar
20x/0.50 Ph 2
Plan-Neofluar
40x/0.75 Ph 2
Plan-Neofluar
100x/1.30 Oil Ph 3
Plan-Apochromat
Plan-Apochromat
40x/1.00 Oil Iris
Plan-Apochromat
63x/1.40 Oil
Plan-Apochromat
100x/1.40 Oil

Objectives

Free working
distance in mm
9.9
4.4
0.53
0.43
0.22
4.4
0.53
0.43
0.22
11.1
4.8
2.07
0.59
0.29
0.29
0.15
0.19
0.19
4.8
2.07
0.59
0.19
9.3
13.6
5.6
1.3
0.47
0.06
5.6
1.3
0.47
0.06
0.31
0.09
0.09
Carl Zeiss
Cover slip
Cat.No.
thickness D in mm
-
441020-0000-000
-
441030-0000-000
0.17
441040-0000-000
0.17
441050-0000-000
0.17
441080-0000-000
-
441031-0000-000
0.17
441041-0000-000
0.17
441051-0000-000
0.17
441081-0000-000
-
440020-0000-000
-
440030-0000-000
0.17
440040-0000-000
0.17
440050-0000-000
0.17
440057-0000-000
0.17
440060-0000-000
0 (no cover slip)
440068-0000-000
0.17
440080-0000-000
0.17
440086-0000-000
-
440031-0000-000
0.17
440041-0000-000
0.17
440051-0000-000
0.17
440081-0000-000
-
440310-0000-000
-
440320-0000-000
0.17
440330-0000-000
0.17
440340-0000-000
0.17
440350-9902-000
0.17
440480-0000-000
0.17
440331-0000-000
0.17
440341-0000-000
0.17
440351-9902-000
0.17
440481-0000-000
0.17
440756-0000-000
0.17
440760-0000-000
0.17
440780-0000-000
D
1-13

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