Zeiss Axioskop 2 plus Operating Manual page 102

OPERATION Axioskop 2 plus
Carl Zeiss Illumination and contrasting techniques Axioskop 2 mot plus
(4) Conclusions
The grayish white color first occurring in the bright position in the above example corresponds to a path
difference of 150 nm, according to the Michel Lévy color chart (3-24).
The "surrounding" of the artificial fiber, which is not birefringent, displays a dark red when the
compensator λ is inserted, which corresponds to the path difference of the compensator of 550 nm
order interference color for the path difference 550 nm, corresponds to 1 λ).
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If the vibration direction of the birefringent specimen to be examined is parallel to the largest vibration
direction of the compensator λ, i.e. in NE-SW direction, the path difference of the specimen (e.g. grayish
white: 150 nm) and the path difference of the compensator λ (red: 550 nm) add up. This results in a
color change of the specimen from grayish white to greenish blue (resulting path difference = 700 nm).
If the vibration direction of the specimen to be examined is perpendicular to the largest vibration
direction of the compensator λ, i.e. in NW-SE direction, the path difference of the specimen (e.g. grayish
white: 150 nm) is subtracted from the path difference of the compensator λ (red: 550 nm). A change in
the interference color of the specimen from grayish white to orange (resulting path difference = 400 nm)
then becomes visible.
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Michel Lévy color charts are available under order No. 42-312.
3-34 B 40-075 e 02/01