Zeiss Axioskop 2 plus Operating Manual page 98

Carl Zeiss
(3) Transmitted-light
Axioskop 2 mot plus
Fig. 3-20 Centering of phase stop on
universal condenser
Fig. 3-21 Centering of phase stop (bright in
condenser) to phase ring (dark in
objective)
3-30
OPERATION
Illumination and contrasting techniques
phase contrast settings on manual
• Swing phase contrast objective, e.g. Ph 1, in the
beam path.
• Insert phase stop labelled like the phase
contrast objective, e.g. Ph 1, using the turret
disk of the universal condenser (with the
Axioskop 2 mot plus
• To check centering and congruence of the
bright phase stop (in condenser) with the dark
phase ring (in objective), remove one eyepiece
from the tube and replace it with the centering
telescope. Use the correction device of the
centering telescope to focus on the phase stop
and the phase ring in the objective exit pupil.
• If congruence is not perfect (3-21/A), the two
SW 1.5 screwdrivers (3-20/1 and 4) must be
used on the two centering screws (3-20/2
and 3) to recenter the bright phase stop until
complete congruence with the dark phase ring
is achieved (3-21/B).
• Insert eyepiece in the tube again.
Normally, however, centering is not required, since
the phase stops are factory-centered and the
centering is retained even if the universal
condenser is removed from the condenser carrier
and attached again.
To enhance the image contrast, an interference
wide-band filter, green 32 x 4, can be placed on
the luminous-field diaphragm or inserted in the
filter mount (if available).
Complete phase contrast is only achieved if the
bright phase stop (in condenser) and the dark
phase ring in objective) are exactly congruent in
the illumination beam path (3-21/B).
Axioskop 2 plus
Axioskop 2 mot plus
Axioskop 2 plus
use keys 3-7/4 and 5).
,
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