Zeiss Axio Vert.A1 Operating Manual
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Zeiss Axio Vert.A1 Operating Manual

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  • Page 2 All the names of firms or products mentioned in this Operating Manual may be trademarks or registered trademarks. Other products are mentioned for information only. Such mention neither constitutes endorsement nor recommendation of those products. Carl Zeiss Microscopy GmbH is not liable for the performance or use of those products. Issued by: Carl Zeiss Microscopy GmbH...

  • Page 3: Table Of Contents

    2.5.2 Axio Vert.A1 FL Stand for Reflected Light and Transmitted Light Fluorescence ....28 2.5.3 Axio Vert.A1 FL-LED Stand for Transmitted Light and Reflected Light LED Fluorescence ..30 2.5.4 Axio Vert.A1 MAT Stand for Reflected Light (Material) ............ 32 Operation and Functional Elements of Optional Components .........
  • Page 4 Changing the Beam-Splitting Mirror in the Intermediate Phototube ......... 67 3.3.6 Mounting the Aqua Stop ....................68 3.3.7 Changing LED Modules in the Axio Vert.A1 FL-LED Stand ..........69 3.3.8 Mounting the Carrier for Transmitted Light Illumination onto the Axio Vert.A1 MAT Stand ....................70 OPERATION .......................
  • Page 5 Property Rights ......................105 LIST OF FIGURES Fig. 1-1 Nameplate on the Axio Vert.A1 FL Stand and Warning Labels on the LED Illuminator for Transmitted Light ......................11 Fig. 1-2 Warning Labels on the Axio Vert.A1 FL-LED Stand ............. 12 Fig.
  • Page 6 Changing the Beam-Splitting Mirror in the Intermediate Phototube ........67 Fig. 3-34 Mounting the Aqua Stop ....................68 Fig. 3-35 Changing the LED Module in the Axio Vert.A1 FL-LED Stand ..........69 Fig. 3-36 Mounting the Carrier for Transmitted Light Illumination ............. 70 Fig. 3-37 Working with LD Objectives ....................

  • Page 7: Axio Vert.a1

    Axio Vert.A1 Table of Contents / List of Figures Carl Zeiss Fig. 4-8 Setting DIC ........................81 Fig. 4-9 Setting Relief Orientation on the iHMC ................83 Fig. 4-10 Centering Screws ......................84 Fig. 4-11 Aligning of the Objective Zone Plate and the iHM Diaphragm ..........85 Fig.

  • Page 8: Introduction

    This symbol indicates a hazard that may arise to the instrument or device system. NOTE This symbol marks a note to which special attention needs to be paid. Be sure to use Axio Vert.A1 inverted microscopes and their original accessories only for the microscoping techniques described in this Operating Manual. 431030-7044-001...
  • Page 9 If any protective measures are found to be no longer effective, put the instrument out of operation and protect it against any inadvertent use. Contact Carl Zeiss Microscopy Service in Germany (see Page 99) or the appropriate Carl Zeiss dealership abroad to get the appliance repaired.
  • Page 10 The instruments must be operated by properly trained personnel only. Such personnel has to be advised of the potential hazards associated with microscoping and the application in question. Axio Vert.A1 microscopes are precision instruments, whose functionality can be impaired or destroyed by improper intervention. Never use this instrument in an explosion hazard environment.

  • Page 11: Nameplate And Warning Labels Attached To The Microscope And To Components

    Axio Vert.A1 Nameplate and Warning Labels Attached to the Microscope and to Components Carl Zeiss Nameplate and Warning Labels Attached to the Microscope and to Components Fig. 1-1 Nameplate on the Axio Vert.A1 FL Stand and Warning Labels on the LED Illuminator for Transmitted Light 05/2012 431030-7044-001...

  • Page 12: Fig. 1-2 Warning Labels On The Axio Vert.a1 Fl-Led Stand

    INTRODUCTION Carl Zeiss Nameplate and Warning Labels Attached to the Microscope and to Components Axio Vert.A1 Fig. 1-2 Warning Labels on the Axio Vert.A1 FL-LED Stand Fig. 1-3 Warning Labels on the VIS-LED (423053-9030-000) 431030-7044-001 05/2012...

  • Page 13: Notes On Warranty

    Repairs may only be carried out by Zeiss Customer Service or by personnel specially authorized by it. Should the instrument malfunction, please contact Carl Zeiss Microscopy Service in Germany first (see Page 99) or get in touch with your Carl Zeiss dealer abroad. 05/2012...

  • Page 14: Types Of Stands (General Views)

    INTRODUCTION Carl Zeiss Axio Vert.A1 Types of Stands (General Views) Types of Stands (General Views) 431030-7044-001 05/2012...

  • Page 15: Instrument Description

    Carl Zeiss INSTRUMENT DESCRIPTION Name, Intended Use Manufacturer’s product name: Axio Vert.A1 Inverted Transmitted Light Microscope Axio Vert.A1 microscopes take the following place within the inverted transmitted light microscope family: Laboratory Microscopes Research Microscopes − Primo Vert − Axio Observer.A1 / .D1 / .Z1 −...

  • Page 16: Instrument Description And Main Features

    The binocular phototubes allow you to connect a microscope camera, reflex camera or a digital / video camera via specific adapters for the purpose of image documentation. Instrument Description and Main Features Axio Vert.A1 microscopes are high-performance desktop instruments available in four microscope stand versions. −...
  • Page 17 Viewing height adjustment Additional intermediate phototube • • Reflected light dark field channel for all objectives (Applies to Axio Vert.A1 MAT only.) • Coded objective nosepiece (Applies to Axio Vert.A1 MAT only.) • Light manager function (Applies to Axio Vert.A1 MAT only.) •...

  • Page 18: System Overview

    INSTRUMENT DESCRIPTION Carl Zeiss System Overview Axio Vert.A1 System Overview 2.3.1 Stands for Biomed Applications Fig. 2-1 Axio Vert.A1 System Overview (Biomed Stands), Page 1 431030-7044-001 05/2012...

  • Page 19: Fig. 2-2 Axio Vert.a1 System Overview (Biomed Stands)

    INSTRUMENT DESCRIPTION Axio Vert.A1 System Overview Carl Zeiss Fig. 2-2 Axio Vert.A1 System Overview (Biomed Stands), Page 2 05/2012 431030-7044-001...

  • Page 20: Fig. 2-3 Axio Vert.a1 System Overview (Biomed Stands)

    INSTRUMENT DESCRIPTION Carl Zeiss System Overview Axio Vert.A1 Fig. 2-3 Axio Vert.A1 System Overview (Biomed Stands), Page 3 431030-7044-001 05/2012...

  • Page 21: Stand For Material Applications

    INSTRUMENT DESCRIPTION Axio Vert.A1 System Overview Carl Zeiss 2.3.2 Stand for Material Applications Fig. 2-4 Axio Vert.A1 System Overview (Material Stand), Page 1 05/2012 431030-7044-001...

  • Page 22: Fig. 2-5 Axio Vert.a1 System Overview (Material Stand)

    INSTRUMENT DESCRIPTION Carl Zeiss System Overview Axio Vert.A1 Fig. 2-5 Axio Vert.A1 System Overview (Material Stand), Page 2 431030-7044-001 05/2012...

  • Page 23: Technical Specifications

    Dimensions Dimensions (Width x Depth x Height) Axio Vert.A1 ..................235 mm x 560 mm x 560 mm Axio Vert.A1 FL or A1 FL-LED ............235 mm x 560 mm x 560 mm Axio Vert.A1 MAT ................. 220 mm x 560 mm...
  • Page 24 LED classification ............ LED risk group 1 according to DIN EN 62471:2009 Fuses According to IEC 127 Axio Vert.A1 microscope stand ..............T 3.15 A/H, 5 mm x 20 mm Power supply unit for HBO 50 ....................T 1.6 A Power supply unit for HBO 100 ..............
  • Page 25 Use of eyepieces up to field number 23 P&C reflector turret .................... Capable of holding four ....................freely loadable P&C reflector modules Change of reflector ........................Manual LED modules ............Four positions freely loadable on Axio Vert.A1 FL-LED 05/2012 431030-7044-001...

  • Page 26: Operation And Functional Elements On The Microscope

    Operation and Functional Elements on the Microscope Axio Vert.A1 Operation and Functional Elements on the Microscope 2.5.1 Axio Vert.A1 Stand for Transmitted Light Legend to Fig. 2-6: Eyepieces Binocular part of the tube Transmitted light illumination (either with a halogen or an LED illuminator)

  • Page 27: Fig. 2-6 Axio Vert.a1

    INSTRUMENT DESCRIPTION Axio Vert.A1 Operation and Functional Elements on the Microscope Carl Zeiss Fig. 2-6 Axio Vert.A1 05/2012 431030-7044-001...

  • Page 28: Axio Vert.a1 Fl Stand For Reflected Light And Transmitted Light Fluorescence

    Carl Zeiss Operation and Functional Elements on the Microscope Axio Vert.A1 2.5.2 Axio Vert.A1 FL Stand for Reflected Light and Transmitted Light Fluorescence Legend to Fig. 2-7: Eyepieces Binocular part of the tube Transmitted light illumination (either with a halogen or an LED illuminator)

  • Page 29: Fig. 2-7 Axio Vert.a1 Fl

    INSTRUMENT DESCRIPTION Axio Vert.A1 Operation and Functional Elements on the Microscope Carl Zeiss Fig. 2-7 Axio Vert.A1 FL 05/2012 431030-7044-001...

  • Page 30: Axio Vert.a1 Fl-Led Stand For Transmitted Light And Reflected Light Led Fluorescence

    INSTRUMENT DESCRIPTION Carl Zeiss Operation and Functional Elements on the Microscope Axio Vert.A1 2.5.3 Axio Vert.A1 FL-LED Stand for Transmitted Light and Reflected Light LED Fluorescence Legend to Fig. 2-8: Eyepieces Binocular part of the tube Transmitted light illumination (either with a halogen or an LED illuminator)

  • Page 31: Fig. 2-8 Axio Vert.a1 Fl-Led

    INSTRUMENT DESCRIPTION Axio Vert.A1 Operation and Functional Elements on the Microscope Carl Zeiss Fig. 2-8 Axio Vert.A1 FL-LED 05/2012 431030-7044-001...

  • Page 32: Axio Vert.a1 Mat Stand For Reflected Light (Material)

    Carl Zeiss Operation and Functional Elements on the Microscope Axio Vert.A1 2.5.4 Axio Vert.A1 MAT Stand for Reflected Light (Material) Legend to Fig. 2-9: Eyepieces Binocular part of the tube 40 mm x 40 mm mechanical stage for reflected light...

  • Page 33: Fig. 2-9 Axio Vert.a1 Mat

    INSTRUMENT DESCRIPTION Axio Vert.A1 Operation and Functional Elements on the Microscope Carl Zeiss Fig. 2-9 Axio Vert.A1 MAT 05/2012 431030-7044-001...

  • Page 34: Operation And Functional Elements Of Optional Components

    INSTRUMENT DESCRIPTION Carl Zeiss Operation and Functional Elements of Optional Components Axio Vert.A1 Operation and Functional Elements of Optional Components 2.6.1 Phototube and Intermediate Phototube Using appropriate adapters, you can attach reflex cameras, microscope cameras and video cameras to the camera port (Fig. 2-10/1 or Fig. 2-11/1) of binocular phototubes.

  • Page 35: Fig. 2-12 Adjusting The Viewing Height On The Binocular Tube

    INSTRUMENT DESCRIPTION Axio Vert.A1 Operation and Functional Elements of Optional Components Carl Zeiss Interpupillary Distance and Viewing Height All the tubes allow the user to − vary the interpupillary distance by adjusting the eyepiece tubes in relation to one another, −...

  • Page 36: Microscope Stages

    INSTRUMENT DESCRIPTION Carl Zeiss Operation and Functional Elements of Optional Components Axio Vert.A1 2.6.2 Microscope Stages Mechanical Stage 130x85 R/L with Short Coaxial Drive − Mechanical stage (Fig. 2-14/1) designed to hold and position specimens in mounting frame K (Fig. 2-14/4) −...

  • Page 37: Condensers

    INSTRUMENT DESCRIPTION Axio Vert.A1 Operation and Functional Elements of Optional Components Carl Zeiss 2.6.3 Condensers To allow a quick condenser change, all the condensers come with a special connection plate for bayonet mounting. The condenser can be rotated in its mount in 90-...

  • Page 38: Objective Nosepiece With Objectives

    DIC contrast or PlasDIC contrast. 2.6.4 Objective Nosepiece with Objectives − Five-fold objective nosepiece (2xH/3xHDIC), provided with M27 thread for five objectives for the Axio Vert.A1, Axio Vert.A1 FL, Axio Vert.A1 FL-LED stands − Five-position objective nosepiece (2xHD/3xHDDIC), coded, with M27 thread for mounting five objectives on the Axio Vert.A1 MAT stand...

  • Page 39: Filter Slider And Slider For Aperture And Luminous Light Diaphragms For The Axio Vert.a1 Mat And Axio Vert.a1 Fl Stands

    Filter Slider and Slider for Aperture and Luminous Light Diaphragms for the Axio Vert.A1 MAT Axio Vert.A1 FL Stands − Reflected light filter slider with three positions for d=25 mm filters (neutral density and color filters, white balance filter). A two- position filter is available as an option.

  • Page 40: Putting The Instrument Into Operation

    PUTTING THE INSTRUMENT INTO OPERATION The Axio Vert.A1 can be set up, changed over and put into operation by the customers themselves. If the customers so request, Zeiss Customer Service will be happy to set up or change over the microscope for a charge.

  • Page 41: Attaching The Binocular (Photo)Tube

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss • Place the microscope stand (Fig. 3-1/3) on a non-vibrating, even, hard and non-flammable worktop. • Remove the plastic cover. • Remove the protective caps (Fig. 3-1/1 and 4).

  • Page 42: Inserting Eyepieces Or An Auxiliary Microscope Or A Diopter

    Make sure the line figure always faces the field stop of the eyepiece. See to it that eyepiece reticles are inserted in dust-free conditions. Reticles should be inserted by Carl Zeiss service personnel only. Fig. 3-4 Inserting an Eyepiece Reticle...

  • Page 43: Screwing Objectives In Place

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss Compensating for Eyesight Differences (e.g., through the Use of Eyepiece Reticles) For the correct use of an eyepiece reticle, two focusable eyepieces are required to ensure that differences in the observer's eyesight can be offset.

  • Page 44: Attaching Microscope Stages

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Standard Components Axio Vert.A1 3.1.6 Attaching Microscope Stages 3.1.6.1 Mounting the Mechanical Stage There are three locating points with retaining screw holes which allow the mechanical stage (mechanical stage 130x85 R/L 40x40 for reflected light) to be mounted directly onto the stand.

  • Page 45: Fig. 3-8 Underside Of The Scanning Stage

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.6.3 Mounting the Scanning Stage To mount scanning stage 130x85 motP; CAN, follow the same procedure as for the mechanical stage. In addition, heed the suggestions below when mounting the scanning stage.

  • Page 46: Fig. 3-10 Terminals On The Underside Of The Scanning Stage

    XY drive (Fig. 3-10/1) and to the PC (Fig. 3-10/2). To enable scanning stage 130x85 motP; CAN to be used on Axio Vert.A1 stands, the stage must be directly connected to a PC with the aid of the CAN-USB converter.

  • Page 47: Inserting, Rotating And Shifting A Condenser

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.7 Inserting, Rotating and Shifting a Condenser The quick-action locking mechanism on the carrier for transmitted light illumination lets you change a condenser easily and fast, with a few turns of your hand.

  • Page 48: Inserting Stop Sliders Into Condensers 0.3 Or 0.4 For Sliders

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Standard Components Axio Vert.A1 Rotating the Condenser in the Transmitted Light Illumination Carrier 90 Degrees • Hold the condenser, so that it cannot move. Rotate the locking ring rightward and push it upward.

  • Page 49: Equipping The Reflector Turret

    Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.9 Equipping the Reflector Turret The reflector turret is fixed-mounted in the Axio Vert.A1 FL, Axio Vert.A1 FL-LED and Axio Vert.A1 MAT stands. Fig. 3-12 Equipping the Reflector Turret • Pull the protective cover (Fig. 3-12/5) off to the right.

  • Page 50: Attaching Transmitted Light Illumination

    Last Checking Step: Insert the three-pin plug of the transmitted light illuminator into the connector socket located at the back of the stand (or into the jacks marked TL on the Axio Vert.A1 MAT) and switch the halogen lamp on.

  • Page 51: Fig. 3-14 Changing The Halogen Lamp For Transmitted Light

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.10.2 Changing the Halogen Lamp for Transmitted Light WARNING When changing the halogen lamp, always be sure to allow for sufficient cooling of the lamp and to pull the power supply plug.

  • Page 52: Attaching Illuminators For Reflected Light

    They can be put together and installed individually by the user. See Section 3.3.7. At the back, the Axio Vert.A1 FL and the Axio Vert.A1 MAT feature a port for the installation of reflected light illuminators. The various stands allow such illuminators as the HBO 50, HBO 100 or HXP 120C to be used for reflected light fluorescence and the VIS-LED attachment lamp or the HAL 100 illuminator to be utilized for material applications.

  • Page 53: Fig. 3-16 Attaching The Hbo 50 Illuminator Or The Hbo 100 Illuminator

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.11.1 Attaching the Fluorescence Illuminator for Reflected Light For safety reasons, the HBO 50/HBO 100 fluorescence lamps and the HXP 120C mercury vapor short-arc lamps are packaged and delivered to the customer separately.

  • Page 54: Fig. 3-17 Adjusting Hbo 50

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Standard Components Axio Vert.A1 3.1.11.2 Adjusting the HBO 50 Illuminator WARNING prevent irreparable damage, never look directly into the ignited lamp. Put on protective glasses, such as sunglasses, to protect your eyes when observing the bright light spot.

  • Page 55: Fig. 3-18 Adjusting Hbo 100

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.11.3 Adjusting the HBO 100 Illuminator The HBO 100 illuminator is available in two versions (manual and automatic adjustment). self-adjustment model automatically adjusted when the power supply unit of the illuminator is switched on.

  • Page 56: Fig. 3-19 Attaching The Vis-Led Illuminator

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Standard Components Axio Vert.A1 3.1.11.4 Installing the VIS-LED Attachment Lamp WARNING Avoid looking directly into the LED light. WARNING Always make sure that the VIS-LED attachment lamp is switched off when mounting it onto or dismounting it from the reflected light port.

  • Page 57: Fig. 3-21 Attaching The Hal 100 Illuminator

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.11.5 Attaching the HAL 100 Illuminator Before using the halogen lamp, be sure to remove the replacement tool for the halogen lamp from the housing, as the tool might otherwise get damaged by the heat generated.

  • Page 58: Adjusting The Hal 100 Illuminator

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Standard Components Axio Vert.A1 3.1.12 Adjusting the HAL 100 Illuminator Coarse Adjustment • Loosen the clamping screw (Fig. 3-21/2) and then detach the operational halogen illuminator (Fig. 3-22/4) from the microscope stand.

  • Page 59: Changing The Hal 100 Halogen Lamp

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Standard Components Carl Zeiss 3.1.13 Changing the HAL 100 Halogen Lamp WARNING Hot surface! You need not remove the lamp housing from the stand to change the halogen lamp. Do not store the replacement tool (Fig.

  • Page 60: Connecting The Microscope To The Power Supply System, Switching The Microscope On And Setting It Up

    If required, use the separate power supply unit to switch on the fluorescence illuminator (e.g., HBO 50 or HBO 100 or HXP 120C) on the Axio Vert.A1 FL stand or the halogen illuminator (HAL 100) on the Axio Vert.A1 MAT. (See Section 3.1.11.1).

  • Page 61: Mounting Optional Components

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Optional Components Carl Zeiss Mounting Optional Components Switch off the microscope and pull the power plug before mounting any optional components. Be sure to put the relevant assemblies back into operational condition after mounting optional components.
  • Page 62 PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Optional Components Axio Vert.A1 • Lock the cams (Fig. 3-25/1) on the underside of the mounting tool into the recesses (Fig. 3-25/4) of the contrast module. • Insert the contrast module into the desired modulator disk position, using the gripping component (Fig.

  • Page 63: Changing Diaphragms In Sliders

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Optional Components Carl Zeiss 3.3.2 Changing Diaphragms in Sliders The positions toward the outside of the slider can each be fitted with Ph phase stops or PlasDIC slit diaphragms. The middle position is fitted with a neutral filter (Fig.

  • Page 64: Changing The Filter Set In The Fl P&C Reflector Module

    (Fig. 3-28/4). This ensures that the wedge angle of the barrier filter will be in the same defined position in the reflector module in use, thus compensating, among modules, for and/or minimizing what is actually a very small image shift anyway in the Zeiss filter sets utilized. 431030-7044-001...

  • Page 65: Changing The Beam Splitter In The Fl P&C Reflector Module

    Please, proceed with utmost care in mounting the filters and the beam splitter to avoid damaging and soiling optical components. We recommend that you order fully fitted FL P&C reflector modules from Carl Zeiss, as changing the beam splitter is a demanding operation. Proceed as follows when changing the beam splitter: •...

  • Page 66: Fig. 3-31 Changing The Beam Splitter

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Optional Components Axio Vert.A1 • Tilt the excitation module half (Fig. 3-31/1), which is now on top, upward and lift it out of the retaining elements (Fig. 3-31/5b) of the lower emission module half (Fig. 3-31/4).

  • Page 67: Changing The Beam-Splitting Mirror In The Intermediate Phototube

    PUTTING THE INSTRUMENT INTO OPERATION Axio Vert.A1 Mounting Optional Components Carl Zeiss 3.3.5 Changing Beam-Splitting Mirror Intermediate Phototube • Put the existing slider (Fig. 3-33/1) of the intermediate phototube on a desk. • Use the SW 2 wrench to loosen the screw, remove the cover (Fig.

  • Page 68: Mounting The Aqua Stop

    PUTTING THE INSTRUMENT INTO OPERATION Carl Zeiss Mounting Optional Components Axio Vert.A1 3.3.6 Mounting the Aqua Stop The aqua stop should be inserted to protect the objectives and the nosepiece whenever you work with liquid specimens. • Detach the microscope stage and screw out the objectives.

  • Page 69: Changing Led Modules In The Axio Vert.a1 Fl-Led Stand

    Make sure that the reflector turret is fitted with filter sets which are suitable for the LED modules used. Reflector turret positions, too, are numbered for that purpose. The LED and the matching filter set need to sit in positions with identical numbers. Fig. 3-35 Changing the LED Module in the Axio Vert.A1 FL-LED Stand 05/2012 431030-7044-001...

  • Page 70: Mounting The Carrier For Transmitted Light Illumination Onto The Axio Vert.a1 Mat Stand

    Mounting the Carrier for Transmitted Light Illumination onto the Axio Vert.A1 MAT Stand If the Axio Vert.A1 MAT stand is to be retrofitted with the optional transmitted light illumination, the carrier for transmitted light illumination must be mounted by the user. If this stand is ordered with transmitted light illumination, the stand will be delivered with the carrier already mounted by the manufacturer.

  • Page 71: Operation

    OPERATION Axio Vert.A1 Use of LD Objectives Carl Zeiss OPERATION Use of LD Objectives In tests performed with inverted microscopes, vessels are usually employed whose bottom thickness differs considerably from the common cover slip thickness of 0.17 mm. Normally, the working distances (a) of low-...

  • Page 72: Use Of Immersion Objectives

    OPERATION Carl Zeiss Use of Immersion Objectives Axio Vert.A1 Use of Immersion Objectives In immersion objectives, the air between the cover slip and the objective is replaced by a liquid, called immersion oil. Put a small bubble-free drop of Immersol 518 N (for transmitted light applications) or 518 F (for fluorescence) on the front lens of the objective.

  • Page 73: Use Of Objectives

    OPERATION Axio Vert.A1 Use of Objectives Carl Zeiss Use of Objectives Objectives for Bright Field and PlasDIC EC Plan-Neofluar 1.25x − EC Plan-Neofluar 2.5x − A-Plan 2.5x − A-Plan 5x − LD A-Plan 10x to 63x (also suitable for phase contrast if available as Ph objective) −...

  • Page 74: Height Stop For The Focusing Drive

    Fig. 4-1 Height Stop of the Focusing Drive Operating Functions on the Axio Vert.A1 FL-LED Stand TL/RL Switch in RL Position: You can set reflected light LED brightness by rotating the illumination intensity control button (Fig. 2-8/14).

  • Page 75: Light Manager Function On The Axio Vert.a1 Mat Stand

    OPERATION Axio Vert.A1 Light Manager Function on the Axio Vert.A1 MAT Stand Carl Zeiss Light Manager Function on the Axio Vert.A1 MAT Stand The light manager function can be employed both in reflected light (using the VIS-LED attachment lamp) and transmitted light (using the HAL illuminator or the LED illuminator for transmitted light, provided the optional carrier for transmitted light illumination is installed).

  • Page 76: Illumination And Contrast Techniques In Transmitted Light

    Techniques in Transmitted Light Every Axio Vert.A1 stand version allows the user to apply the transmitted light methods described below (Axio Vert.A1 MAT with optional transmitted light illumination only). 4.11.1 Transmitted Light Bright Field Requirements − The microscope must have properly been put into operation, as described in Section 3.

  • Page 77: Transmitted Light Phase Contrast

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss 4.11.2 Transmitted Light Phase Contrast Requirements − The microscope must have properly been put into operation, as described in Section 3. − The microscope must be switched on.
  • Page 78 OPERATION Carl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A1 • Use the relevant centering screws (Fig. 4-3/2) on the slider to line up the phase ring and the phase stop. Make sure that the gray phase ring of the objectives fully covers the bright phase stop (Fig.

  • Page 79: Transmitted Light Varel Contrast

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss 4.11.3 Transmitted Light VAREL Contrast VAREL (variable relief contrast) delivers relief-like images of specimens and can be used as an alternative to phase contrast. VAREL may also be employed for curved surfaces, e.g., 96-microtiter plates, which cannot be contrasted with phase contrast, as it is impossible to set a ring overlap.

  • Page 80: Transmitted Light Differential Interference Contrast (Dic)

    OPERATION Carl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A1 Moving the VAREL illumination all the way to a position outside of the pupil corresponds to unilateral DF illumination. Moving the VAREL illumination between the Ph and VAREL rings of the objective corresponds to oblique HF illumination.

  • Page 81: Fig. 4-8 Setting Dic

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss Setting DIC • Open the aperture diaphragm on the condenser completely. Put on the specimen. On the nosepiece, rotate in the objective suitable for DIC. • • Put the corresponding DIC slider (Fig. 4-8/3) into the slot on the nosepiece. Make sure the DIC slider snaps safely into position.

  • Page 82: Transmitted Light Plasdic

    OPERATION Carl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A1 4.11.5 Transmitted Light PlasDIC PlasDIC, an innovative interference contrast, delivers relief-like images and is to be used for thicker specimens, in particular. The contrast is variably adjustable. The cavities of microtiter plates can be contrasted right to the edge.

  • Page 83: Transmitted Light Ihmc

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss 4.11.6 Transmitted Light iHMC iHMC (improved Hoffman modulation contrast) is an optimized version of the HMC contrast technique. It produces a relief image of the specimen viewed. Much like PlasDIC, this type of contrast has established itself as the standard in the IVF (in-vitro fertilization) area, in particular.

  • Page 84: Fig. 4-10 Centering Screws

    OPERATION Carl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A1 • If necessary, use the two centering keys to precenter the iHMC module in the mount, based on your visual judgment. The two holes for accessing the centering screws (Fig. 4-10/1) are located under the change position.

  • Page 85: Fig. 4-11 Aligning Of The Objective Zone Plate And The Ihm Diaphragm

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss Aligning the iHMC Module • Rotate the iHMC objective into the light path. Put on the microscope a typical specimen, such as an egg cell or adherent cells, placed in a cultivation •...

  • Page 86: Transmitted Light Polarization Contrast

    Setting the Polarization on the Axio Vert.A1 MAT, Using Polarizer DL and Analyzer Slider A 12x35 mm, Rotatable 90 Degrees • Set up the microscope for bright field in transmitted light.

  • Page 87: Illumination And Contrast Techniques In Reflected Light

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Reflected Light Carl Zeiss 4.12 Illumination and Contrast Techniques in Reflected Light 4.12.1 Reflected Light Bright Field Reflected light bright field microscopy is the simplest and most common optical microscopy technique designed to examine opaque specimens or objects, such as polished material faces or wafers.

  • Page 88: Fig. 4-14 Setting Bright Field

    OPERATION Carl Zeiss Illumination and Contrast Techniques in Reflected Light Axio Vert.A1 • If required, adjust the illumination intensity on the On/off control button (Fig. 4-14/4) or place a neutral-density filter into the filter slider (Fig. 4-14/5) and slide it into the light path.

  • Page 89: Reflected Light Dark Field

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Reflected Light Carl Zeiss 4.12.2 Reflected Light Dark Field The reflected light dark field technique is used when not entirely reflecting surfaces with differences in reflectivity (ideal bright field objects) are examined, but scratches, cracks and pores exist - in short, when there are deviations from plane surfaces.

  • Page 90: Reflected Light Dic

    OPERATION Carl Zeiss Illumination and Contrast Techniques in Reflected Light Axio Vert.A1 4.12.3 Reflected Light DIC The reflected light DIC or reflected light C-DIC technique (DIC = differential interference contrast; C-DIC = differential interference contrast in circularly polarized light) allows a high-contrast imaging of phase objects, i.e., objects which, unlike amplitude objects, only change the phase of the light.
  • Page 91 OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Reflected Light Carl Zeiss Setting C-DIC • Set the microscope up for reflected light bright field. Rotate the C-DIC P&C reflector module into the light path. • • Insert C-DIC slider 6x20 (Fig. 4-15/1) into the slot.

  • Page 92: Reflected Light Polarization

    OPERATION Carl Zeiss Illumination and Contrast Techniques in Reflected Light Axio Vert.A1 4.12.4 Reflected Light Polarization Reflected light polarization is another contrasting option for polished faces of ore minerals, coal, ceramic products, certain metals and metal alloys, as those often exhibit a different reflection behavior in linearly polarized light, depending on the orientation of the crystals or object details.

  • Page 93: Fig. 4-16 Setting The Polarization

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Reflected Light Carl Zeiss Setting Polarization with P&C Reflector Module and Analyzer Slider A 12x35 mm, Rotatable 90 Degrees • Set up the microscope for bright field in reflected light. • Rotate in the Pol P&C reflector module on the reflector turret.

  • Page 94: Reflected Light Fluorescence

    Once you have selected the area of the specimen, use the on/off function (Fig. 4-17/5 and 6) to • switch off the transmitted light illumination (Fig. 4-17/1). When you employ the Axio Vert.A1 FL-LED stand, the TL/RL switch (Fig. 2-8/20) lets you shift directly between transmitted light and reflected light. •...

  • Page 95: Fig. 4-17 Setting Fluorescence

    OPERATION Axio Vert.A1 Illumination and Contrast Techniques in Reflected Light Carl Zeiss • Insert the FL attenuator into slot A for aperture sliders. Set the correct brightness with the adjustment wheel. • Additional excitation filters measuring 25 mm in diameter and held in position by O-rings can be inserted into the additional filter slider (Fig.

  • Page 96: Micromanipulators

    Micromanipulators Axio Vert.A1 4.13 Micromanipulators Axio Vert.A1 microscopes come prepared for the use of micromanipulators. For this purpose, there are two tapped holes each to the right (Fig. 4-18/1) and left of the carrier for transmitted light illumination, which are covered by protective caps.

  • Page 97: Care, Maintenance, Troubleshooting And Service

    Clean the optical surface, using a circular motion from the middle outward to the edge of the surface. In so doing, apply light pressure on the optical surface. Heed the following suggestions when using the Axio Vert.A1 in regions with a hot and humid climate: •...

  • Page 98: Maintenance

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Carl Zeiss Maintenance Axio Vert.A1 Maintenance 5.2.1 Performing Checks The following checks should be performed at six-month intervals: General • Check the power cable and power plug for possible damage. • Switch off and secure the instrument if you detect any damage. Be sure to have defects repaired by Zeiss Service.

  • Page 99: Service

    In order to ensure that your microscope stays optimally adjusted and operates smoothly for a longer period of time, we recommend that you sign a service / maintenance contract with Carl Zeiss. Whenever you require service, contact your regional dealer or...

  • Page 100: Annex

    ANNEX Carl Zeiss List of Abbreviations Axio Vert.A1 ANNEX List of Abbreviations A / AL Auflicht = reflected light Alternating current BioMed. Biological-medical Brillenträger-Eignung = Suitable for spectacle wearers Canadian Standards Association (Canadian standards and testing agency) Deckglasdicke = cover slip thickness...

  • Page 101: Keyword Index

    ANNEX Axio Vert.A1 Keyword Index Carl Zeiss Keyword Index Page Adjusting the HAL 100 ........................58 Adusting the HBO 100 Illuminator ....................... 55 Adusting the HBO 50 Illuminator ......................54 Ambient conditions ..........................23 Analyzer ..........................26, 28, 30, 32 Analyzer slider ........................32, 92, 93 Aperture diaphragm ...........................
  • Page 102 ANNEX Carl Zeiss Keyword Index Axio Vert.A1 ECO ..........................26, 28, 30, 32, 73 Equipping the reflector turret .......................49 Ergotube .............................35 Eye deficiency .............................43 Eyepiece reticle............................42 Eyepieces ........................26, 28, 30, 32, 42 Filter set ..............................64 Filter slider ........................26, 28, 30, 32 Filter slider reflected light ........................39...
  • Page 103 ANNEX Axio Vert.A1 Keyword Index Carl Zeiss Main feature ............................16 Maintenance ............................98 Mechanical stage .......................28, 32, 36, 44 Micromanipulators ..........................96 Microscope stage ......................26, 28, 32, 36, 44 Modulator disk ........................37, 38, 61 Mounting frame ..........................36 Mounting the Aqua Stop ........................
  • Page 104 ANNEX Carl Zeiss Keyword Index Axio Vert.A1 Scanning stage ............................45 Screwing objectives in .........................43 Service ..............................99 Setting the microscope ........................60 Setup ..............................40 Shutter plate ..........................52, 76 Slider ............................37, 39 Specimen guide ...........................30 Specimen stage ........................26, 30, 44 Stand ........................

  • Page 105: Property Rights

    ANNEX Axio Vert.A1 Property Rights Carl Zeiss Property Rights The instruments, components or processes described in this manual are protected by the following patents: − See the label attached to the microscope stand. 05/2012 431030-7044-001...

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