Quantitative Microscopy; Measurement Of Lengths - Zeiss Axioskop 2 plus Operating Manual

Carl Zeiss
3.7

Quantitative microscopy

3.7.1

Measurement of lengths

The measurement of lengths using the Axioskop 2 plus / Axioskop 2 mot plus requires the following, for
example:
− stage micrometer, positive 5 + 100/100 y, D = 0.17 mm as the object
− eyepiece crossline micrometer 10:100, d = 26 mm in the eyepiece
An overview of available stage micrometers and eyepiece reticles is given in section 1.7.
Before length measurement using the microscope can be performed, the micrometer or scale value of the
objective / eyepiece reticle combination must be determined. This scale value is exactly that distance in
the specimen which complies to one interval of the used crossline micrometer.
For calibration, align the scales of the stage micrometer and the crossline micrometer parallel to each
other by turning the eyepiece, and make the zero line of both scales exactly congruent. If, for example,
99 increments (of 10 µm each) of the stage micrometer correspond to exactly 100 increments of the
crossline micrometer, as in Fig. 3-32, the resulting scale value k' for the used objective / eyepiece reticle
combination (A-Plan 10x/0.25 and crossline micrometer 10:100) is
99
k' = x 10 µm = 9.9 µm
100
☞
The distance to be measured should be ≥ 5 mm in the eyepiece field to keep the influence of
random measuring deviations as low as possible. Other measuring errors can occur if the
eyepiece has not been inserted into the tube until stop.
3-50
OPERATION
Quantitative microscopy
Axioskop 2 plus
Axioskop 2 mot plus
B 40-075 e 02/01