Zeiss Axioscope 7 Operating Manual
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Zeiss Axioscope 7 Operating Manual

Upright microscope for routine and entry-level research
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Summary of Contents for Zeiss Axioscope 7

  • Page 1 Operating Manual Axioscope 5/7/Vario Microscope for Routine and Entry-Level Research Upright Microscope for Routine and Entry-level Research...
  • Page 2 Third party products are cited for information purposes only and this does not represent approval or recommendation of these products. Carl Zeiss Microscopy GmbH assumes no liability for the performance or use of such products. Published by Carl Zeiss Microscopy GmbH...

  • Page 3: Table Of Contents

    2.4.4 Axioscope 5 stand, Pol-TL/RL ..................26 2.4.5 Axioscope 5 stand, Mat-TL/RL ..................28 2.4.6 Axioscope 7 stand, Mat-TL/RL mot.................. 30 2.4.7 Axioscope 5 Vario material stand ..................32 2.4.8 Functions of stands keys and display elements ..............34 Controls and functional elements on microscope components .........
  • Page 4 3.1.9 Mechanical stages with friction adjustment..............52 3.1.10 Attaching the Pol rotary stage ..................53 3.1.11 Mounting/removing the 80x60 motorized mechanical stage on the Axioscope 7 motorized material stand ....................56 3.1.12 Mounting the condenser carrier ..................57 3.1.13 Mounting the condenser ....................57 3.1.14...
  • Page 5 Axioscope 5/7/Vario Contents / List of Illustrations ZEISS 4.2.2 Setting up transmitted light darkfield microscopy using the KÖHLER method ....90 4.2.3 Setting up transmitted light phase contrast microscopy ........... 93 4.2.4 Setting up transmitted light differential interference contrast (DIC)microscopy ....95 4.2.5...
  • Page 6 Warning label on microscopes with Colibri 3 light source for reflected light ......15 Fig. 1-5 Warning label on Axioscope 7 stand using the motorized 80x60 mechanical stage 80x60 ... 15 Fig. 2-1 Interface diagram (example: Axioscope 5 stand, Bio-TL/RL) ..........20 Fig.
  • Page 7 Axioscope 5/7/Vario Contents / List of Illustrations ZEISS Fig. 3-20 Plugging in the power cable of the motorized mechanical stage ........56 Fig. 3-21 Mounting the condenser carrier ..................57 Fig. 3-22 Mounting the condenser ....................57 Fig. 3-23 Mounting the darkfield condenser ..................58 Fig.
  • Page 8 ZEISS Contents / List of Illustrations Axioscope 5/7/Vario Fig. 4-8 Components for the transmitted light DIC method ............. 96 Fig. 4-9 Components for transmitted light polarization ..............99 Fig. 4-10 Determination of the polarization direction n ' in a synthetic fiber ........100 γ...

  • Page 9: Introduction

    INTRODUCTION Axioscope 5/7/Vario Notes on instrument safety ZEISS INTRODUCTION Notes on instrument safety The Axioscope 5/7/Vario microscopes were engineered, manufactured and tested in accordance with the DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 safety standards for electrical measuring, control and laboratory equipment.
  • Page 10 Whenever it becomes apparent that any of the safety mechanisms are out of order, the microscope must be switched off and protected from any inadvertent use. Please contact the ZEISS Service Department or the Carl Zeiss Microscopy Service before switching the microscope on again.
  • Page 11 INTRODUCTION Axioscope 5/7/Vario Notes on instrument safety ZEISS CAUTION Only authorized personnel are permitted to operate the instruments. The personnel must have been instructed about and be aware of the risks involved in using the microscope. The Axioscope is a precision instrument whose functionality can easily be damaged or even destroyed when handled improperly.
  • Page 12 INTRODUCTION ZEISS Notes on instrument safety Axioscope 5/7/Vario CAUTION  The immersion fluid Immersol 518 N is irritating to the skin. Avoid any contact with skin,   eyes and clothes. Read the safety data sheets on Immersol 518 N , Immersol 518 F ...

  • Page 13: Warning Labels On The Microscopes

    INTRODUCTION Axioscope 5/7/Vario Warning labels on the microscopes ZEISS ATTENTION Always position equipment in a manner that allows easy disconnection from the power supply. ATTENTION Do not use a power supply cord with an inadequate rating. ATTENTION When using the HAL or HBO illuminators, a base plate for the Axioscope (000000-2202-526) is mandatory.

  • Page 14: Fig. 1-2 Warning Labels On Microscopes With Hal 100 For Transmitted Light And Hbo 100 For

    INTRODUCTION ZEISS Warning labels on the microscopes Axioscope 5/7/Vario Fig. 1-2 Warning labels on microscopes with HAL 100 for transmitted light and HBO 100 for reflected light Fig. 1-3 Warning label on microscopes with LED light source for reflected light...

  • Page 15: Fig. 1-4 Warning Label On Microscopes With Colibri 3 Light Source For Reflected Light

    INTRODUCTION Axioscope 5/7/Vario Warning labels on the microscopes ZEISS Fig. 1-4 Warning label on microscopes with Colibri 3 light source for reflected light Fig. 1-5 Warning label on Axioscope 7 stand using the motorized 80x60 mechanical stage 80x60 03/2018 430035-7344-001...

  • Page 16: Notes On The Warranty

    Only ZEISS service personnel or personnel authorized especially by ZEISS may perform repair work on the microscopes. In the event of malfunction of your instrument, please contact the ZEISS Microscopy Service Department (see page 131) or the ZEISS agency assigned to your country.

  • Page 17: Description Of The Instrument

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Intended use ZEISS DESCRIPTION OF THE INSTRUMENT Intended use The Axioscope 5/7/Vario microscopes are universal microscopes designed especially for applications in biological and medical fields and for materials analysis. Depending on the configuration of the microscope stand, they may be used with transmitted light only or with a combination of transmitted light and reflected light.

  • Page 18: Technical Data

    Mains frequency ........................50 to 60 Hz Power consumption of the Axioscope 5 with internal power supply unit ....................60 VA Power consumption of the Axioscope 7 with internal power supply unit ....................100 VA Power consumption of the Axioscope Vario with external power supply unit ....................
  • Page 19 DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Technical data ZEISS HBO 100 W power supply unit Range of application ........................indoor Protection class ............................I Protection type ..........................IP 20 Mains voltage ......................100 VAC ... 240 VAC ....................... No voltage adjustment required Mains frequency ........................

  • Page 20: Interface Diagram

    DESCRIPTION OF THE INSTRUMENT ZEISS Interface diagram Axioscope 5/7/Vario Interface diagram The following figure shows a diagram of the microscope’s hardware interfaces. The Axioscope 5 stand Bio-TL/RL with LED/HAL illumination serves as the example. Fig. 2-1 Interface diagram (example: Axioscope 5 stand, Bio-TL/RL)

  • Page 21: Controls And Functional Elements On The Microscope

    3. Axioscope 5 stand, Bio-TL/RL (430035-9061-000) 4. Axioscope 5 stand, Pol-TL (430035-9261-000) 5. Axioscope 5 stand, Pol-TL/RL (430035-9291-000) 6. Axioscope 5 stand, Mat-TL/RL (430035-9121-000) 7. Axioscope 7 stand, Mat-TL/RL, motorized (430035-9330-000) 8. Axioscope 5 Vario material stand (430035-9150-000) 03/2018 430035-7344-001...

  • Page 22: Axioscope 5 Stands, Bio-Tl

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.2 Axioscope 5 stands, Bio-TL Transmitted light stands for bioscience equipped with the following main components: 1. Version 1 (430035-9032-000): HAL 50 halogen illumination Version 2 (430035-9201-000): LED 10 W-TL illumination or optionally HAL 100 halogen illumination using an external power supply 2.

  • Page 23: Fig. 2-2 Controls And Functional Elements Of The Axioscope 5 Stands, Bio-Tl

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Fig. 2-2 Controls and functional elements of the Axioscope 5 stands, Bio-TL 03/2018 430035-7344-001...

  • Page 24: Axioscope 5 Stand, Bio-Tl/Rl

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.3 Axioscope 5 stand, Bio-TL/RL Transmitted and reflected light fluorescence stand for bioscience equipped with the following main components: 1. LED 10 W-TL illumination or optionally with HAL 100 halogen...

  • Page 25: Fig. 2-3 Controls And Functional Elements Of The Axioscope 5 Stand, Bio-Tl/Rl

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Fig. 2-3 Controls and functional elements of the Axioscope 5 stand, Bio-TL/RL 03/2018 430035-7344-001...

  • Page 26: Axioscope 5 Stand, Pol-Tl/Rl

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.4 Axioscope 5 stand, Pol-TL/RL Transmitted and reflected light stand for polarization equipped with the following main components: 1. LED 10 W-TL illumination or optionally with HAL 100 halogen...

  • Page 27: Fig. 2-4 Controls And Functional Elements Of The Axioscope 5 Stand, Pol-Tl/Rl

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Fig. 2-4 Controls and functional elements of the Axioscope 5 stand, Pol-TL/RL 03/2018 430035-7344-001...

  • Page 28: Axioscope 5 Stand, Mat-Tl/Rl

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.5 Axioscope 5 stand, Mat-TL/RL Transmitted and reflected light stand for material equipped with the following main components: 1. LED 10 W-TL illumination or optionally with HAL 100 halogen illumination using an external power supply for transmitted light applications 2.

  • Page 29: Fig. 2-5 Controls And Functional Elements Of The Axioscope 5 Stand, Mat-Tl/Rl

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Fig. 2-5 Controls and functional elements of the Axioscope 5 stand, Mat-TL/RL 03/2018 430035-7344-001...

  • Page 30: Axioscope 7 Stand, Mat-Tl/Rl Mot

    Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.6 Axioscope 7 stand, Mat-TL/RL mot. Transmitted and reflected light stand for material equipped with the following main components: 1. LED 10 W-TL illumination or optionally with HAL 100 halogen illumination using an external power supply for transmitted light applications 2.

  • Page 31: Fig. 2-6 Controls And Functional Elements Of The Axioscope 7 Stand, Mat-Tl/Rl Mot

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Fig. 2-6 Controls and functional elements of the Axioscope 7 stand, Mat-TL/RL mot. 03/2018 430035-7344-001...

  • Page 32: Axioscope 5 Vario Material Stand

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.7 Axioscope 5 Vario material stand Transmitted and reflected light stand for material, equipped with the following main components: 1. Upper stand part including reflected-light illumination LED 10 W, with interface for HAL / HBO / HXP 2.

  • Page 33: Fig. 2-7 Controls And Functional Elements Of The Axioscope 5 Vario Material Stand

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Fig. 2-7 Controls and functional elements of the Axioscope 5 Vario material stand 03/2018 430035-7344-001...

  • Page 34: Functions Of Stands Keys And Display Elements

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on the microscope Axioscope 5/7/Vario 2.4.8 Functions of stands keys and display elements For the location of the keys and display elements on your stand, refer to sections 2.4.2 - 2.4.7.
  • Page 35 DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on the microscope ZEISS Availability Action Functionality/Description Left Snap Short press *: Snaps an image button (see Fig. 2-5/22) Starts video recording; another short press is required Long press **: (if Axiocam to stop recording.

  • Page 36: Controls And Functional Elements On Microscope Components

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on microscope components Axioscope 5/7/Vario Controls and functional elements on microscope components 2.5.1 Binocular photo tube 30°/23 (100:0/0:100) The photo port ( F ig. 2-8/2) of the binocular tube 7 1 0 H...

  • Page 37: Binocular Ergo Tube/Ergo Photo Tube 20°/23 And Ergo Photo Tube 15°/23, Each With Continuous Vertical Adjustment

    The camera port can carry a maximum weight of Fig. 2-9 Binocular ergo photo tube 20°/23 2.5 kg (camera plus cable). For a fee, the ZEISS with vertical adjustment service personnel will equip your instrument with a higher maximum load, if needed.

  • Page 38: Nosepiece With Objectives

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on microscope components Axioscope 5/7/Vario 2.5.3 Nosepiece with objectives − Equipped with DIN slot ( F ig. 2-11/3) for 7 0 3 H compensators or fluorescence protection shield − M27 mounting thread for six objectives −...

  • Page 39: Low-Power System For 2.5X/4X Objectives Mounted On The Condenser Carrier

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on microscope components ZEISS 2.5.5 Low-power system for 2.5x/4x objectives mounted on the condenser carrier The low-power system enables full illumination of the field of view when using a low-magnification objective (2.5x-4x) in combination with the...

  • Page 40: 14X40 Mm Stop Sliders For Aperture And Field Diaphragms

    DESCRIPTION OF THE INSTRUMENT ZEISS Controls and functional elements on microscope components Axioscope 5/7/Vario 2.5.7 14x40 mm stop sliders for aperture and field diaphragms To adjust the reflected light beam path using the KÖHLER method, one 14x40 mm stop slider F ig.

  • Page 41: Reflector Turrets With 4 Or 6 Coded Positions

    DESCRIPTION OF THE INSTRUMENT Axioscope 5/7/Vario Controls and functional elements on microscope components ZEISS 2.5.8 Reflector turrets with 4 or 6 coded positions These reflector turrets are equipped with four or six coded push-and-click (P&C) reflector positions. Turn the knurled wheel to adjust the reflector position.

  • Page 42: Start-Up

    START-UP The Axioscope microscopes can be installed, modified and started up by the customer himself. On request, the ZEISS Service installs or modifies the microscope for an extra charge. NOTE Before installing and starting-up the microscope, read the Notes on instrument safety (see section 1.1) carefully and thoroughly.

  • Page 43: Mounting The Axioscope 5 Vario Material Upper Stand Part On The Stand Column

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.2 Mounting the Axioscope 5 Vario material upper stand part on the stand column ATTENTION Equipment or parts having a mass of 18 kg or more shall be provided with a means for careful lifting and carrying.

  • Page 44: Mounting The Binocular Tube/Photo Tube

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.3 Mounting the binocular tube/photo tube • Loosen the screws ( F ig. 3-3/3) with the Allen 2 9 5 H wrench (AF 3). • Remove the dust cover caps ( F ig. 3-3/2 and 5)

  • Page 45: Fig. 3-5 Inserting The E-Pl 10X/23 Gw Foc. Pol Eyepieces Into The Binocular Photo Tube With Upright Image

    (Fig. 3-6/W), but the red dot (Fig. 3-6/R). NOTE The eyepiece reticles must be inserted under dust-free conditions. This should be carried out only by ZEISS Service. 03/2018 430035-7344-001...

  • Page 46: Screwing In Objectives

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario Inserting reversible eyecups The eyepieces have rubber protection rings to avoid scratches on eyeglasses. These may be replaced by reversible eyecups as desired. • To do this, remove the eyeglass protection rings (Fig. 3-6/2) from the eyepieces and mount the eyecups (Fig.

  • Page 47: Inserting And Removing Push-And-Click (P&C) Reflector Modules Into The Reflector Insert

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.6 Inserting and removing push-and-click (P&C) reflector modules into the reflector insert To install or uninstall the modules, the reflector insert (reflector turret with 4 or 6 coded positions, reflector slider with 2 coded positions) must be removed from the upper part of the stand.

  • Page 48: Mounting The Reflector Insert

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.7 Mounting the reflector insert • First, remove the cover cap ( F ig. 3-9/1) by 3 2 2 H inserting the Allen wrench (AF 3) into the slot F ig. 3-9/2). Turn...

  • Page 49: Fig. 3-11 Adjusting The X- And Y-Axis Knob Tension

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS b) How to mount the stage • Fit the stage ( F ig. 3-10/1) onto the stage carrier ( F ig. 3-10/6) so that the threaded holes in the bottom 3 2 9 H...

  • Page 50: Fig. 3-13 Centering The Rotatable Mechanical Stage

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario b) How to mount the stage • Loosen the screw caps ( F ig. 3-12/2) of the spring box with approx. 3 turns. 3 3 8 H • Put the stage with the dovetail ring notch onto the spring pin ( F ig.

  • Page 51: Fig. 3-14 Adjusting Ergonometric Drive

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS (4) Removing and mounting the additional sleeves Both coaxial knurled knobs for the stages are equipped with additional sleeves for an even more sensitive adjustment of the specimen position. These sleeves can be removed in those cases when it is important to shift the specimen more quickly.

  • Page 52: Mechanical Stages With Friction Adjustment

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.9 Mechanical stages with friction adjustment The drive length and friction for the X- and Y-adjustment can be adjusted individually on the standard mechanical stage (432035-9002-000) and the mechanical stage 432035-9070-000. (1) Adjusting the drive length on the stage...

  • Page 53: Attaching The Pol Rotary Stage

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.10 Attaching the Pol rotary stage Two rotary stage models are available. The mounting procedure is the same for each model. 3.1.10.1 Removing the Pol rotary stage • Loosen the screw cap (Fig. 3-16/8) of spring pin with about 3 turns.

  • Page 54: Fig. 3-17 Centering The Pol Rotary Stage

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.10.3 Removing the attachable Pol object guide and mounting stage clips • Turn the control wheel (Fig. 3-16/3) of the Pol object guide (Fig. 3-16/1) until the clamping screw becomes visible in the mounting hole (Fig. 3-16/2).

  • Page 55: Fig. 3-18 Centering Objectives

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.10.6 Centering objectives of polarizing microscope stands The nosepiece with 5-positions HF/DF/Pol and 1-position HF/DF/DIC equipped with five centerable objective mounts (without DIC slots) as well as one objective mount with DIC slot (non- centerable).

  • Page 56: Mounting/Removing The 80X60 Motorized Mechanical Stage On The Axioscope 7 Motorized Material Stand

    Axioscope 5/7/Vario 3.1.11 Mounting/removing the 80x60 motorized mechanical stage on the Axioscope 7 motorized material stand The motorized stage can only be mounted on/removed from the stand as an entire unit with the stage carrier (see section 3.1.15 on page 59).

  • Page 57: Mounting The Condenser Carrier

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.12 Mounting the condenser carrier • Adjust the guide of the condenser carrier F ig. 3-21/3) using the coaxial knurled knob 3 9 1 H F ig. 3-21/2) until both screws ( F ig. 3-21/4)

  • Page 58: Mounting The Darkfield Condenser

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario NOTE Follow the same procedure when mounting other condensers. NOTE If an additional component, e.g. a polarizer, has been mounted beneath the condenser carrier, the stage carrier should be removed. NOTE If the Abbe condenser is used in combination with a rotary stage, ensure that the position of the condenser head is centered as exactly as possible relative to the stage opening.

  • Page 59: Mounting The Stage Carrier

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.15 Mounting the stage carrier • Screw the shoulder screw ( F ig. 3-24/3) into the 4 0 5 H appropriate opening: − Opening with mark 0 ( F ig. 3-24/3): 4 0 6 H No sample space extension is mounted.

  • Page 60: Changing The 12 V, 50 W Halogen Lamp Of The Hal 50 Halogen Illuminator

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.16 Changing the 12 V, 50 W halogen lamp of the HAL 50 halogen illuminator • Switch off the microscope and disconnect it from the power supply. Wait at least 15 minutes for the halogen lamp to cool down.

  • Page 61: Mounting The Illuminator Tl Led10 Cri90/Rl Led10 Cri90

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.17 Mounting the illuminator TL LED10 CRI90/RL LED10 CRI90 • Using the Allen wrench (AF 3) (Fig. 3-27/7), loosen the clamping screw (Fig. 3-27/6) of the TL illumination connector (Fig. 3-27/1) or the RL illumination connector (Fig. 3-27/2) at the lower back of the stand.

  • Page 62: Mounting The Axioscope Base Plate On The Stand

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.18 Mounting the Axioscope base plate on the stand NOTE When using the HAL or HBO illuminators, a base plate for Axioscope (000000-2202-526) is mandatory. Fig. 3-28 Mounting the base plate • Remove the four rubber feet (Fig. 3-28/1) from the bottom of the stand.

  • Page 63: Mounting And Adjusting The Hal 100 Halogen Illuminator

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.19 Mounting and adjusting the HAL 100 halogen illuminator The HAL 100 halogen illuminator may serve as a light source for transmitted light and reflected light procedures (except fluorescence light) as long as either the lower or upper part of the stand is equipped with the appropriate connection piece.
  • Page 64 START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.19.2 Adjusting the HAL 100 Coarse setting • After loosening the clamping screw ( F ig. 3-29/1 4 2 8 H F ig. 3-29/9), remove the ready-to-operate 4 2 9 H halogen illuminator ( F ig.
  • Page 65 START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.19.3 Changing the halogen lamp 12 V, 100 W CAUTION Hot surface! Danger of burns! Always let the illuminator case cool off for at least 15 min before touching it! NOTE It is not necessary to remove the illuminator case from the stand when replacing the halogen lamp.

  • Page 66: Inserting The Adjustment Tool Of Hbo 100 Into The Stand Bio-Tl/Rl

    START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.20 Inserting the adjustment tool of HBO 100 into the stand Bio-TL/RL • Remove the cover ( F ig. 3-32/1) from the 4 4 7 H mounting aperture of the adjustment tool. • Insert the adjustment tool ( F ig.
  • Page 67 START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.21.2 Mounting the HBO 100 illuminator • Remove the cover from the reflected light connection piece ( F ig. 3-33/1). 4 7 4 H • Insert the illuminator case ( F ig. 3-33/3) with the...
  • Page 68 START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.21.3 Adjusting the HBO 100 illuminator The HBO 100 illuminator is available in two versions (manual and automatic adjustment). The self-adjusting HBO 100 (423011-9901-000) adjusts automatically when the power supply unit is turned on.

  • Page 69: Mounting The Colibri 3 Illumination System And Changing The Led Modules

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS 3.1.22 Mounting the Colibri 3 illumination system and changing the LED modules 3.1.22.1 Mounting the Colibri 3 illumination system on the upper part of the Axioscope stand CAUTION Before removing the Colibri 3 from the microscope stand, please make sure the main power is switched off.
  • Page 70 START-UP ZEISS Mounting standard components Axioscope 5/7/Vario 3.1.22.2 Changing the LED modules in the Colibri 3 illumination system  How to change an LED module, i.e. the LED module for LED slot 1 (Fig. 3-38/ • Remove the Colibri 3 from the stand: Follow the instructions of section 3.1.22.1 in reverse order.

  • Page 71: Mounting The External Illumination Fixture Hxp 120

    START-UP Axioscope 5/7/Vario Mounting standard components ZEISS Usability of LED modules for Colibri 3 Wavelength 385 nm LED 470 nm LED 505 nm LED 565 nm LED 625 nm LED Position range 423052-9593-000 423052-9573-000 423052-9562-000 423052-9602-000 423052-9522-000 Slot 1 450~480 nm...

  • Page 72: Mounting Optional Components

    START-UP ZEISS Mounting optional components Axioscope 5/7/Vario Mounting optional components CAUTION Always disconnect the stand and the external power supply units from the power mains before beginning any mounting work. 3.2.1 Mounting the intermediate plate for Analyzer Slider • Loosen the screw ( F ig.

  • Page 73: Mounting The Tube Lens Turret

    START-UP Axioscope 5/7/Vario Mounting optional components ZEISS 3.2.2 Mounting the tube lens turret Mounting the tube lens turret is done similarly to mounting the intermediate plate for analyzer sliders. See section 3.2.1. NOTE Before using the tube lens turret, please remove the tube lens from the used tube.

  • Page 74: Changing The Filters In The Reflector Module Fl P&C

    5 3 3 H in the reflector modules acts equally and compensates or minimizes the image offset – already low in ZEISS filter sets – even further. When mounting a filter without any orientation marks (arrow), we recommend proceeding as follows: Fig.

  • Page 75: Changing The Color Splitter In The Reflector Module Fl P&C

    START-UP Axioscope 5/7/Vario Mounting optional components ZEISS 3.2.5 Changing the color splitter in the reflector module FL P&C ATTENTION Be extremely careful when installing the filters and the color splitter to avoid damaging and soiling the optical components. We recommend ordering completely equipped reflector modules FL P&C, as changing the color...

  • Page 76: Mounting The Polarizer D Or The Filter Holder

    START-UP ZEISS Mounting optional components Axioscope 5/7/Vario NOTE The reflective (coated) side ( F ig. 3-45/3) 5 5 0 H of the color splitter has a tapered edge F ig. 3-45/1) or corner ( F ig. 3-45/2). 5 5 1 H 5 5 2 H •...

  • Page 77: Mounting And Centering The Low-Power System For 2.5X/4X Objectives

    START-UP Axioscope 5/7/Vario Mounting optional components ZEISS 3.2.7 Mounting and centering the low- power system for 2.5x/4x objectives • If necessary, remove the polarizer or filter holder from the condenser carrier. • Hold the low-power system ( F ig. 3-47/4) parallel...

  • Page 78: Inserting The Modulator Disk In The 0.9/1.25 Bf Condenser

    START-UP ZEISS Mounting optional components Axioscope 5/7/Vario 3.2.8 Inserting the modulator disk in the 0.9/1.25 BF condenser • Remove the condenser ( F ig. 3-48/1) from the 5 8 0 H condenser carrier (see section 3 .1.13). 5 8 1 H If the condenser cannot be lowered sufficiently, e.g.

  • Page 79: Changing The Plasdic Diaphragm

    START-UP Axioscope 5/7/Vario Mounting optional components ZEISS 3.2.9 Changing the PlasDIC diaphragm How to mount the slit diaphragm (426715-9000- 000): • Turn the PhC diaphragm that needs to be replaced into the free aperture of the modulator disk. • Screw the modulator disk’s centering screws F ig.

  • Page 80: Changing The Filter In The Filter Wheel Transmitted Light

    START-UP ZEISS Mounting optional components Axioscope 5/7/Vario 3.2.11 Changing the filter in the filter wheel transmitted light • Remove the stage carrier (see section 3 .1.12). 5 9 9 H • Unscrew three screws ( F ig. 3-51/3) of the field...

  • Page 81: Connecting To The Power Supply And Switching The Microscope On/Off

    START-UP Axioscope 5/7/Vario Connecting to power supply and … ZEISS Connecting to the power supply and switching the microscope on/off 3.3.1 Microscope stand • Connect the microscope’s power supply ( F ig. 3-52/2) with its power cord to an electrical outlet.

  • Page 82: Connecting The Hal 100 Halogen Illuminator For Transmitted Light

    START-UP ZEISS Connecting to power supply and … Axioscope 5/7/Vario 3.3.2 Connecting the HAL 100 halogen illuminator for transmitted light NOTE When using the HAL illuminator, a base plate for Axioscope (000000-2202-526) is mandatory. • Mount the HAL 100 ( F ig.

  • Page 83: Switching The Illumination On/Off

    START-UP Axioscope 5/7/Vario Connecting to power supply and … ZEISS 3.3.4 Switching the illumination on/off • Turn the power switch ( F ig. 3-52/1) of the 4 9 7 H microscope with the lower part of the stand for LED or HAL 50 illumination either on or off.

  • Page 84: Using The Light Manager Function

    START-UP ZEISS Using the Light Manager function Axioscope 5/7/Vario Using the Light Manager function When turned on, the previous setting of the Light Manager will be restored. The LM function is enabled automatically if the default factory settings are active.

  • Page 85: Operation

    OPERATION Axioscope 5/7/Vario Default setting of the microscope ZEISS OPERATION Default setting of the microscope 4.1.1 Setting the inter-pupillary distance on the binocular tube • Adjust the individual inter-pupillary distance by symmetrically swiveling of the two eyepiece tubes towards each other ( F ig.

  • Page 86: Adjusting For Ametropia (User's Visual Impairment) When Using Eyepiece Reticles

    OPERATION ZEISS Default setting of the microscope Axioscope 5/7/Vario 4.1.3 Adjusting for ametropia (user's visual impairment) when using eyepiece reticles The prerequisite for the correct use of an eyepiece reticle is two adjustable eyepieces (e.g. PL 10x/23 GW foc.) to compensate for different degrees of ametropia of the user.

  • Page 87: Illumination And Contrast Methods In Transmitted Light Microscopy

    OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS Illumination and contrast methods in transmitted light microscopy 4.2.1 Setting up transmitted light brightfield microscopy using the KÖHLER method (1) General principle of operation Of all the optical microscopy methods, transmitted light brightfield microscopy is the one most commonly used.
  • Page 88 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario • Center the field diaphragm image with both centering screws ( F ig. 4-3/4) on the condenser 6 2 0 H carrier ( F ig. 4-3/C). 6 2 1 H •...
  • Page 89 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS (4) Adjusting height stop condenser carrier • Loosen the setscrew ( F ig. 4-4/1) on the height 6 2 5 H stop with the Allen wrench (AF 3).

  • Page 90: Setting Up Transmitted Light Darkfield Microscopy Using The Köhler Method

    OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario 4.2.2 Setting up transmitted light darkfield microscopy using the KÖHLER method (1) General principle of operation Uncolored biological samples, like bacteria or living cell cultures, are sometimes hard to see in transmitted light brightfield microscopy due to their translucence.
  • Page 91 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS NOTE Objectives with built-in iris apertures have apertures which are too high for the transmitted light darkfield microscopy. Stop the iris aperture down at least until it reaches the border aperture for the darkfield of the condenser in use.
  • Page 92 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario (5) Setting darkfield contrast with immersion oil darkfield condenser • If necessary, swivel out the low-power system, polarizer or λ plate. • Move the condenser carrier down until the end stop.

  • Page 93: Setting Up Transmitted Light Phase Contrast Microscopy

    OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS 4.2.3 Setting up transmitted light phase contrast microscopy (1) General principle of operation The phase contrast method is ideal for examining thin uncolored samples, e.g. culture cells. Generally, the human eye cannot detect phase differences (variations in refractive index or thickness) within the different cell components.
  • Page 94 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario • If the overlap is not exact ( F ig. 4-7/A), the 6 3 2 H lighter annular diaphragm must be re-centered with the aid of two Allen wrenches (AF 1.5) F ig.

  • Page 95: Setting Up Transmitted Light Differential Interference Contrast (Dic)Microscopy

    OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS 4.2.4 Setting up transmitted light differential interference contrast (DIC)microscopy (1) General principle of operation The transmitted light DIC method allows for a high-contrast vivid display of transparent sample details.
  • Page 96 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario DIC slider Knurled screw Objective on the nosepiece Condenser with DIC prism Compensator λ Analyzer module Reflector turret Fig. 4-8 Components for the transmitted light DIC method 430035-7344-001...

  • Page 97: Setting Up Transmitted Light Plasdic Contrast Microscopy

    OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS 4.2.5 Setting up transmitted light PlasDIC contrast microscopy (1) General principle of operation PlasDIC can be used independently from the material of the specimen holder. The contrast method gives a relief-like image and is especially well suited for thicker objects. The contrast is adjustable.

  • Page 98: Setting Transmitted Light Polarization

    OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario 4.2.6 Setting transmitted light polarization 4.2.6.1 Detecting birefringence (1) Application The transmitted light polarization method is used for samples which change the polarization of the light. Such samples are called birefringent. Examples include crystals, minerals or polymers. If such birefringent substances are observed between crossed polarizers, the birefringent portion of the sample appears bright while its surroundings remain dark.
  • Page 99 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS Fig. 4-9 Components for transmitted light polarization NOTE If an analyzer slider is going to be used for working with the Axioscope, either the reflector module H P&C or an empty position must be swiveled in on the reflector turret/slider.
  • Page 100 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario 4.2.6.2 Determination of the polarization direction nγ' (1) Application The determination of the polarization direction of n or n respectively (polarization direction with the γ γ' absolute or relative largest index of refraction) and n...
  • Page 101 Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS • Now insert the analyzer back into the beam path and remove the adjustment sample. The forward direction of the polarizer and analyzer is now parallel to the cross hair in the reticle (polarizer east-west, analyzer north-south).
  • Page 102 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario (4) Conclusions According Michel-Lévy color table F ig. 4-11), the gray-white color which occurs in 6 5 6 H the bright position as discussed in the example above (Fig. 4-10/1) corresponds to a path difference of 150 nm.
  • Page 103 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS 4.2.6.3 Measuring path differences Measuring compensators are required for the accurate measurement of path differences,. These compensators reset, i.e. compensate the path difference produced by the specimen to zero (first order black).
  • Page 104 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario − A dark gray appearing as the interference color suggests very small path differences (λ/10 or 54.6 nm). The compensator appropriate for this application: Rotary compensator Brace–Köhler λ/10 (Accessory 000000-1115-703).
  • Page 105 Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS • Then, swivel the top part of the D circular polarizer (Fig. 4-12/2) into the light path. • Rotate the lever of the λ/4 plate of the D circular polarizer (Fig. 4-12/1) until obtaining maximum extinction (dark-gray field of view) (lever points 45°...

  • Page 106: Setting Transmitted Light Polarization For Conoscopic Observation - Determining The Optical Character Of Crystals

    OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario 4.2.7 Setting transmitted light polarization for conoscopic observation - determining the optical character of crystals For the classification (and thus identification) of crystalline matter, the examination of the interference image in the objective pupil delivers more valuable information than that obtained by viewing the specimen itself.
  • Page 107 Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS (2) Setting the microscope for simple conoscopy using the diopter or the auxiliary microscope • Set the microscope as for transmitted light brightfield microscopy using the KÖHLER method (see also section 4.2.1).
  • Page 108 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario (2) Setting the microscope for conoscopy using the Bertrand system module • Set the microscope as for transmitted light brightfield microscopy using the KÖHLER method(see also section 4.2.1).
  • Page 109 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS 4.2.7.4 Conoscopy with intermediate plate and Bertrand lens slider (1) Microscope configuration − Recommended strain-free, high-quality objectives: EC Plan-Neofluar 40x/0.9 Pol objective or EC Plan-Neofluar 100x/1.30 Oil Pol objective or EC Epiplan-Neofluar 50x/0.8 Pol objective or...
  • Page 110 OPERATION ZEISS Illumination and contrast methods in transmitted light … Axioscope 5/7/Vario • Swivel in the recommended objective and focus with the focusing drive. • Close the luminous-field diaphragm as far as necessary to prevent the axial figures of adjacent crystals from being superimposed on the axial figure.
  • Page 111 Axioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS If the positions of the section are less favorable, i.e. the center of the cross of optically uniaxial or the isogyre of optically biaxial specimens is outside the objective pupil, the optical character can be assessed as follows: •...

  • Page 112: Illumination And Contrast Methods In Reflected Light Microscopy

    OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario Illumination and contrast methods in reflected light microscopy 4.3.1 Setting up reflected light brightfield microscopy using the KÖHLER method (1) Application Reflected light brightfield microscopy is the easiest and most commonly used optical microscopy method.
  • Page 113 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in reflected light … ZEISS • Turn the knurled button of the aperture diaphragm ( F ig. 4-15/2) to a medium position (approx. half 6 6 2 H open and half closed). • Adjust the knurled knob on the field diaphragm ( F ig.
  • Page 114 OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario Centering screw of the aperture diaphragm Knurled button of the aperture diaphragm Centering screw of the field diaphragm Knurled wheel of the field diaphragm Focusing drive Nosepiece Fig. 4-15...

  • Page 115: Setting The Reflected Light Darkfield

    OPERATION Axioscope 5/7/Vario Illumination and contrast methods in reflected light … ZEISS 4.3.2 Setting the reflected light darkfield (1) Application The reflected light darkfield method is applied when samples are examined which do not have areas with different reflectivity (ideal brightfield samples), but which show scratches, cracks, pores (i.e., deflections) on the plane surface.

  • Page 116: Setting Reflected Light Dic And Reflected Light C-Dic

    OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario 4.3.3 Setting reflected light DIC and reflected light C-DIC (1) Application The reflected light DIC and the reflected light C-DIC methods (DIC = Differential Interference Contrast; C-DIC = Differential Interference Contrast in circular polarized light) are used for the high-contrast imaging of phase objects, i.e.

  • Page 117: Setting Reflected Light Tic

    OPERATION Axioscope 5/7/Vario Illumination and contrast methods in reflected light … ZEISS 4.3.4 Setting reflected light TIC (1) Application The reflected light TIC method (Micro-interferometry; TIC = Total Interference Contrast in the circular polarized light) is used in imaging and measuring specimen structures which are on hand in different azimuths.
  • Page 118 OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario The values a and b (see F ig. 4-18) are determined 6 8 4 H with the aid of the eyepiece reticle micrometer or with the micrometer eyepiece.
  • Page 119 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in reflected light … ZEISS Attention: • If the step and its surroundings are made from different materials, the phase jumps characteristic for the material must be considered. For all non-conducting materials, the phase jump is 180°, and for all semi-conductors only slightly different from 180°.

  • Page 120: Setting Reflected Light Polarization - Proof Of Bireflectance And Reflection Pleochroism

    OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario 4.3.5 Setting reflected light polarization – proof of bireflectance and reflection Pleochroism (1) Application Reflected light polarization is a contrasting method suited for cut surfaces of mineral ore, coal, ceramics, special metals and alloys.
  • Page 121 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in reflected light … ZEISS A sample is bireflectant when the sample details show differences in brightness and color which change when the stage rotates. For samples with low bireflectance we recommend using the analyzer with a rotatable lambda plate.

  • Page 122: Setting Up Reflected Light Fluorescence

    OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario 4.3.6 Setting up reflected light fluorescence (1) General principle of operation The reflected light fluorescence method is used to show fluorescent substances in typical fluorescent colors in high contrast. The light originating from a high-performance illuminator in a reflected light fluorescence microscope passes through a heat protection filter onto an excitation filter (bandpass).
  • Page 123 OPERATION Axioscope 5/7/Vario Illumination and contrast methods in reflected light … ZEISS Fig. 4-20 Components for reflected light fluorescence • Keep the light path in the reflected light illuminator blocked via the elimination position of the filter slider for reflected light ( F ig.
  • Page 124 OPERATION ZEISS Illumination and contrast methods in reflected light … Axioscope 5/7/Vario • Remove an eyepiece from the tube and adjust the aperture diaphragm by sight. • Open the aperture diaphragm ( F ig. 4-20/3) enough to see the whole objective exit pupil.

  • Page 125: Care, Fuse Replacement And Service

    CARE, FUSE REPLACEMENT AND SERVICE Axioscope 5/7/Vario Instrument care ZEISS CARE, FUSE REPLACEMENT AND SERVICE Instrument care Maintenance of the Axioscope is limited to the following activities: • Turn the instrument off after each use and cover it with the appropriate protection cover (dust and humidity protection).

  • Page 126: Instrument Maintenance

    CARE, FUSE REPLACEMENT AND SERVICE ZEISS Instrument maintenance Axioscope 5/7/Vario Instrument maintenance 5.2.1 Checking the instrument • Be sure to comply with the required voltage (e.g. on the power supply unit for HBO 100). • Check the power cable and the power plug for damage.

  • Page 127: Replacing The Fuses In The 12 Vdc 100 W Power Supply Unit

    CARE, FUSE REPLACEMENT AND SERVICE Axioscope 5/7/Vario Instrument maintenance ZEISS 5.2.3 Replacing the fuses in the 12 VDC 100 W power supply unit CAUTION Disconnect the instrument from the power supply before handling the instrument! If fuses blow, the cause must be identified and any technical defect must be properly repaired.

  • Page 128: Troubleshooting

    CARE, FUSE REPLACEMENT AND SERVICE ZEISS Troubleshooting Axioscope 5/7/Vario Troubleshooting 5.3.1 Microscope Problem Cause Solution Shading or brightness irregularities The vis/phot push-pull rod/shift knob Move the vis/phot push-pull rod/shift in the field of view of the on the photo tube is not in correct knob to the correct functional position microscope;...
  • Page 129 XY stage stops at wrong position The XY stage initialization failed. Restart the microscope, if the issue after initialization on Axioscope 7 still exists please contact ZEISS Service. Cannot focus on the sample under The Z-axis resolution was not...
  • Page 130 CARE, FUSE REPLACEMENT AND SERVICE ZEISS Troubleshooting Axioscope 5/7/Vario Problem Cause Solution The left and the right field of view Distance of the eyepiece (distance of Re-adjust the distance of the eyepiece, cannot be brought together in one the pupils) is not adjusted correctly.

  • Page 131: Maintenance And Repair Work

    Maintenance and repair work Maintenance and repair work on the mechanical, optical and electronic parts inside the instrument and on the electrical equipment of the Axioscope microscopes may be performed only by the ZEISS Service Department or specially authorized technical personnel.

  • Page 132: Annex

    ANNEX ZEISS List of abbreviations Axioscope 5/7/Vario ANNEX List of abbreviations Alternating Current Automatic Component Recognition Width across flats A-Plan Achromatic objectives with improved flatness of field (ICS optical products) B/BF Bright field Charge-coupled Device Canadian Standards Association C-DIC Differential Interference Contrast in circular polarized light...
  • Page 133 ANNEX Axioscope 5/7/Vario List of abbreviations ZEISS Ph/PhC Phase Contrast Plan PlasDIC Differential Interference Contrast for Plastic Receptacles Polarization P&C Push&Click Right-hand (coaxial knurled knob on the right-hand side of the mechanical stage) Reflected light Single Lens Reflex Slow (type of fuse)

  • Page 134: Index

    Axioscope 5 stand, Bio-TL/RL .......................24 Axioscope 5 stand, Mat-TL/RL ......................28 Axioscope 5 stand, Pol-TL/RL ........................26 Axioscope 5 Vario material stand ....................32, 43 Axioscope 7 stand, Mat-TL/RL mot.......................30 Base plate ............................32 Bertrand lens ............................. 106 Bertrand lens slider ..........................109 Bertrand system ..........................
  • Page 135 ANNEX Axioscope 5/7/Vario Index ZEISS Darkfield ............................ 90, 115 Default factory settings ........................84 Default setting ............................ 85 Depolarizer ............................98 Description of the instrument ......................17 Diaphragm ..........................79, 87, 94 DIC ..............................116 Differential Interference Contrast ......................95 Dimensions ............................
  • Page 136 ANNEX ZEISS Index Axioscope 5/7/Vario Instrument safety ..........................9 Intended use ............................17 Interface diagram ..........................20 Intermediate plate ..........................72 Inter-pupillary distance ........................85 Keys ..............................34 Knurled wheel for switching the reflector turret position ..........24, 26, 28, 30, 32 KÖHLER ..........................87, 90, 95 Light intensity .......................
  • Page 137 ANNEX Axioscope 5/7/Vario Index ZEISS Reflected light (RL) button ....................24, 26, 28, 30 Reflected light brightfield ........................112 Reflected light fluorescence ......................122 Reflected light illuminator ..................24, 26, 28, 30, 32, 123 Reflector insert ........................... 48 Reflector module ..........................47 Reflector slider ............................

  • Page 138: Industrial Property Rights

    ANNEX ZEISS Industrial property rights Axioscope 5/7/Vario Warranty .............................16 Weight ...............................18 Industrial property rights The instruments, their components and the methods described in this manual are protected by the following patents: − US 6154282 − US 6392796 − US 6595661 −...
  • Page 139 [ Camera with SLR bayonet ] Upper stand part Axioscope 7, HAL 100 / HBO, 6x HD, DIC, M27 Axiocam 202 mono Camera Adapter T2-C 1" 1.0x 426570-9010-000 Adapter Video 426104-0000-000 Axiocam 208 color 60 C 1/3" 0.4x Camera Adapter T2-C 1" 1.0x,...
  • Page 140 Pol ACR P&C analyzer module DIC sliders DIC slider C 6x20 for 424937-9901-000 432333-9001-000 432039-9200-000 EC EPN 50x-100x/LD EC EPN 100x (only for use with Axioscope 7) 000000-1105-193 Optovar modules TIC slider 6x20 Further specimen holders Lightguide HXP 120 with liquid fill (on request) 000000-1105-190 (see price list 40.04.04)

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