Zeiss Axioskop 2 plus Operating Manual page 100

Carl Zeiss
3.3.4.2 Determination of the vibration direction n
(1) Application
The determination of vibration directions n γ , n γ' (direction with the absolutely and relatively highest
refractive index), n α and n α' (direction with the absolutely and relatively lowest refractive index) in
relation to morphological directions, e.g. of crystal surfaces, crystal needles or fibers, is an important
criterion for recognition. It is also used for the diagnosis of biocrystals (e.g. gout, pseudogout).
Fig. 3-23 Determination of the vibration direction n
(2) Axioskop 2 plus and Axioskop 2 mot plus configuration
− Eyepiece with reticle
− Objectives suitable for polarization
− Rotary mechanical stage 75x50/240° R (3-22/2)
− SENARMONT DIC polarizer or polarizer D (rotary or fixed), unless a condenser with integrated polarizer
is used.
− Analyzer module D in reflector turret
− Adjusting specimen for polarization microscopy (453679-0000-000)
(3) Setting of Axioskop 2 plus and Axioskop 2 mot plus
• Set the microscope as for transmitted-light brightfield (see section 3.3.1 (3)), taking care to ensure
that the interpupillary distance of the binocular tube has been correctly set (see section 2.1.3 (2)).
• Center the rotary mechanical stage (3-22/2) (see section 2.2.9 (2)).
• Swing polarizer (3-22/1) in beam path and position it to 0°, provided that a rotary polarizer is used.
• Swing in analyzer module on reflector turret (3-22/3). The field of view now appears dark on account
of the crossed polarizers.
• Place Pol adjusting specimen on the microscope stage and turn the stage until the adjusting specimen
is in dark position.
• Switch off analyzer and align crosslines with the cracks of the object.
• Then switch on analyzer again and remove the adjusting specimen. The transmission directions of
polarizer and analyzer are now parallel to the crosslines (polarizer E-W, analyzer N-S).
3-32
OPERATION
Illumination and contrasting techniques
γ '
γ '
using the example of an artificial fiber
Axioskop 2 plus
Axioskop 2 mot plus
B 40-075 e 02/01